Gene description for PPIH
Gene name peptidylprolyl isomerase H (cyclophilin H)
Gene symbol PPIH
Other names/aliases CYP-20
CYPH
SnuCyp-20
USA-CYP
Species Homo sapiens
 Database cross references - PPIH
ExoCarta ExoCarta_10465
Vesiclepedia VP_10465
Entrez Gene 10465
HGNC 14651
MIM 606095
UniProt O43447  
 PPIH identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Thymus 23844026    
 Gene ontology annotations for PPIH
Molecular Function
    peptidyl-prolyl cis-trans isomerase activity GO:0003755 IBA
    peptidyl-prolyl cis-trans isomerase activity GO:0003755 IDA
    protein binding GO:0005515 IPI
    cyclosporin A binding GO:0016018 IBA
    ribonucleoprotein complex binding GO:0043021 IDA
    RNA polymerase II CTD heptapeptide repeat P3 isomerase activity GO:0140839 IEA
    RNA polymerase II CTD heptapeptide repeat P6 isomerase activity GO:0140840 IEA
Biological Process
    mRNA splicing, via spliceosome GO:0000398 IC
    regulation of transcription by RNA polymerase II GO:0006357 IEA
    protein folding GO:0006457 IBA
    positive regulation of viral genome replication GO:0045070 IMP
    protein-containing complex assembly GO:0065003 TAS
Subcellular Localization
    nucleoplasm GO:0005654 TAS
    spliceosomal complex GO:0005681 IC
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 TAS
    nuclear speck GO:0016607 IDA
    intracellular membrane-bounded organelle GO:0043231 IBA
    U4/U6 x U5 tri-snRNP complex GO:0046540 IDA
    U4/U6 snRNP GO:0071001 IDA
 Experiment description of studies that identified PPIH in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PPIH
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 BPIFA1 51297
Two-hybrid Homo sapiens
2 RPL26L1 51121
Affinity Capture-MS Homo sapiens
3 XIAP  
Two-hybrid Homo sapiens
4 PAXIP1  
Affinity Capture-MS Homo sapiens
5 Prpf8 192159
Affinity Capture-MS Mus musculus
6 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
7 DOK3 79930
Two-hybrid Homo sapiens
8 PBK  
Co-fractionation Homo sapiens
9 LSM4 25804
Affinity Capture-MS Homo sapiens
10 BRD2  
Affinity Capture-MS Homo sapiens
11 UBC 7316
Affinity Capture-MS Homo sapiens
12 PRPF3  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
13 N4BP2L2 10443
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
14 PTPN1 5770
Co-fractionation Homo sapiens
15 SNRPF 6636
Affinity Capture-MS Homo sapiens
16 SNRPD2 6633
Affinity Capture-MS Homo sapiens
17 XRCC1 7515
Affinity Capture-MS Homo sapiens
18 SNRPC 6631
Affinity Capture-MS Homo sapiens
19 Tagap  
Affinity Capture-MS Mus musculus
20 ABI1 10006
Co-fractionation Homo sapiens
21 PPM1A 5494
Co-fractionation Homo sapiens
22 ITGA4 3676
Affinity Capture-MS Homo sapiens
23 UCHL5 51377
Co-fractionation Homo sapiens
24 VCAM1 7412
Affinity Capture-MS Homo sapiens
25 PFN2 5217
Co-fractionation Homo sapiens
26 METTL18  
Affinity Capture-MS Homo sapiens
27 MYCN  
Affinity Capture-MS Homo sapiens
28 RBM42  
Affinity Capture-MS Homo sapiens
29 NIF3L1 60491
Two-hybrid Homo sapiens
30 WDR1 9948
Co-fractionation Homo sapiens
31 RBMX 27316
Co-fractionation Homo sapiens
32 PPP1CA 5499
Co-fractionation Homo sapiens
33 FOXA1  
Affinity Capture-MS Homo sapiens
34 PRPF8 10594
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
35 DDX21 9188
Affinity Capture-MS Homo sapiens
36 MRE11A 4361
Co-fractionation Homo sapiens
37 MEPCE 56257
Affinity Capture-MS Homo sapiens
38 KLC1 3831
Co-fractionation Homo sapiens
39 RPS10 6204
Co-fractionation Homo sapiens
40 SRSF3 6428
Co-fractionation Homo sapiens
41 TRIM24  
Affinity Capture-MS Homo sapiens
42 PPIH 10465
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
43 SNRNP200 23020
Co-fractionation Homo sapiens
44 CD2BP2 10421
Co-fractionation Homo sapiens
45 RAD18  
Affinity Capture-MS Homo sapiens
46 PRPF18  
Two-hybrid Homo sapiens
47 PRPF31 26121
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
48 CEBPA  
Protein-peptide Homo sapiens
49 ST13 6767
Co-fractionation Homo sapiens
50 HEATR6  
Co-fractionation Homo sapiens
51 HNRNPD 3184
Affinity Capture-MS Homo sapiens
52 RPL32 6161
Affinity Capture-MS Homo sapiens
53 OLA1 29789
Affinity Capture-MS Homo sapiens
54 RBM39 9584
Affinity Capture-MS Homo sapiens
55 LSM8 51691
Affinity Capture-MS Homo sapiens
56 IPO5 3843
Co-fractionation Homo sapiens
57 USP4 7375
Affinity Capture-MS Homo sapiens
58 PRPF4B 8899
Affinity Capture-MS Homo sapiens
59 USP15 9958
Affinity Capture-MS Homo sapiens
60 BAG2 9532
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
61 SNRPB 6628
Affinity Capture-MS Homo sapiens
62 UBQLN2 29978
Two-hybrid Homo sapiens
63 CUL3 8452
Affinity Capture-MS Homo sapiens
64 MECP2 4204
Affinity Capture-MS Homo sapiens
65 EGFR 1956
Negative Genetic Homo sapiens
66 FUS 2521
Affinity Capture-MS Homo sapiens
67 FN1 2335
Affinity Capture-MS Homo sapiens
68 SF3A1 10291
Affinity Capture-MS Homo sapiens
69 EFTUD2 9343
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
70 MYC  
Affinity Capture-MS Homo sapiens
71 TOP1 7150
Affinity Capture-MS Homo sapiens
72 NHP2L1 4809
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
73 SART3 9733
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
74 DDRGK1 65992
Affinity Capture-MS Homo sapiens
75 HUWE1 10075
Affinity Capture-MS Homo sapiens
76 SEC23IP 11196
Co-fractionation Homo sapiens
77 GRB2 2885
Co-fractionation Homo sapiens
78 CPSF6 11052
Affinity Capture-MS Homo sapiens
79 PCMT1 5110
Co-fractionation Homo sapiens
80 MKI67  
Affinity Capture-MS Homo sapiens
81 RAD50 10111
Co-fractionation Homo sapiens
82 LSM7  
Affinity Capture-MS Homo sapiens
83 ALYREF 10189
Affinity Capture-MS Homo sapiens
84 ITPA 3704
Co-fractionation Homo sapiens
85 CIT 11113
Affinity Capture-MS Homo sapiens
86 LSM6 11157
Affinity Capture-MS Homo sapiens
87 PRPF4 9128
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
88 C9orf72  
Affinity Capture-MS Homo sapiens
89 SSRP1 6749
Affinity Capture-MS Homo sapiens
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