Gene description for DNM1L
Gene name dynamin 1-like
Gene symbol DNM1L
Other names/aliases DLP1
DRP1
DVLP
DYMPLE
EMPF
HDYNIV
Species Homo sapiens
 Database cross references - DNM1L
ExoCarta ExoCarta_10059
Vesiclepedia VP_10059
Entrez Gene 10059
HGNC 2973
MIM 603850
UniProt O00429  
 DNM1L identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 34887515    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Embryonic kidney cells 36706192    
Embryonic kidney cells 36706192    
Foreskin fibroblasts 34108659    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Lymphoma cells 34108659    
Mammary cancer-associated fibroblasts 34108659    
Mesenchymal stem cells 36408942    
Monocytic leukemia cells 34108659    
Normal mammary epithelial cells 34108659    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic duct epithalial cells 34108659    
Pancreatic duct epithalial cells 34108659    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Pluripotent stem cells 34108659    
T lymphocytes 34108659    
Thymus 23844026    
 Gene ontology annotations for DNM1L
Molecular Function
    GTPase activity GO:0003924 IBA
    GTPase activity GO:0003924 IDA
    GTPase activator activity GO:0005096 IC
    protein binding GO:0005515 IPI
    GTP binding GO:0005525 IEA
    microtubule binding GO:0008017 IBA
    lipid binding GO:0008289 IEA
    GTP-dependent protein binding GO:0030742 IDA
    small GTPase binding GO:0031267 IDA
    ubiquitin protein ligase binding GO:0031625 IPI
    identical protein binding GO:0042802 IPI
    protein homodimerization activity GO:0042803 IDA
    membrane scission GTPase motor activity GO:1990606 IEA
Biological Process
    mitochondrial fission GO:0000266 IBA
    mitochondrial fission GO:0000266 IDA
    mitochondrial fission GO:0000266 IMP
    mitochondrial fission GO:0000266 IMP
    calcium ion transport GO:0006816 IEA
    endocytosis GO:0006897 IBA
    mitochondrion organization GO:0007005 IMP
    mitochondrion organization GO:0007005 IMP
    endoplasmic reticulum organization GO:0007029 IMP
    regulation of gene expression GO:0010468 IEA
    peroxisome fission GO:0016559 IBA
    peroxisome fission GO:0016559 IDA
    peroxisome fission GO:0016559 IMP
    mitochondrial fragmentation involved in apoptotic process GO:0043653 IBA
    mitochondrial fragmentation involved in apoptotic process GO:0043653 IMP
    intracellular distribution of mitochondria GO:0048312 IBA
    intracellular distribution of mitochondria GO:0048312 IMP
    rhythmic process GO:0048511 IEA
    positive regulation of protein secretion GO:0050714 IDA
    protein complex oligomerization GO:0051259 IMP
    heart contraction GO:0060047 IEA
    protein localization to mitochondrion GO:0070585 IEA
    protein localization to mitochondrion GO:0070585 IMP
    positive regulation of neutrophil chemotaxis GO:0090023 IMP
    positive regulation of mitochondrial fission GO:0090141 IMP
    positive regulation of mitochondrial fission GO:0090141 TAS
    mitochondrial membrane fission GO:0090149 IDA
    mitochondrial membrane fission GO:0090149 IMP
    mitocytosis GO:0160040 IEA
    regulation of peroxisome organization GO:1900063 IEA
    regulation of mitophagy GO:1901524 IGI
    regulation of ATP metabolic process GO:1903578 IEA
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 IMP
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IBA
    mitochondrion GO:0005739 IDA
    mitochondrion GO:0005739 IMP
    mitochondrial outer membrane GO:0005741 IDA
    mitochondrial outer membrane GO:0005741 TAS
    peroxisome GO:0005777 IDA
    peroxisome GO:0005777 IMP
    endoplasmic reticulum GO:0005783 IDA
    endoplasmic reticulum GO:0005783 IDA
    endoplasmic reticulum membrane GO:0005789 TAS
    Golgi apparatus GO:0005794 IDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 IMP
    cytosol GO:0005829 TAS
    microtubule GO:0005874 IDA
    microtubule GO:0005874 IDA
    brush border GO:0005903 IEA
    clathrin-coated pit GO:0005905 IEA
    membrane GO:0016020 HDA
    membrane GO:0016020 IBA
    synaptic vesicle membrane GO:0030672 IEA
    protein-containing complex GO:0032991 IDA
    intracellular membrane-bounded organelle GO:0043231 IDA
    perinuclear region of cytoplasm GO:0048471 IDA
    mitochondrion-derived vesicle GO:0099073 IDA
 Experiment description of studies that identified DNM1L in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 412
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX|ACTB
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MCF7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
6
Experiment ID 414
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
7
Experiment ID 426
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 427
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 207
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 208
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
11
Experiment ID 209
MISEV standards
✘ Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
12
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
13
Experiment ID 407
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|CD81|SDCBP|TSG101|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
14
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
15
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
16
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
17
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
18
Experiment ID 1275
MISEV standards
Biophysical techniques
✔
CD9|TSG101|FLOT1|FLOT2|RAB35|RAB5A|GAPDH|TFRC|CD63|Alix
Enriched markers
✔
PCNA|CANX|GAPDH|HSP90AA1|HSP90B1
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36706192    
Organism Homo sapiens
Experiment description Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles
Authors "Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q."
Journal name Sci Adv
Publication year 2023
Sample Embryonic kidney cells
Sample name HEK293T
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
19
Experiment ID 1280
MISEV standards
Biophysical techniques
✔
TSG101|CD9|FLOT1|FLOT2|RAB35|RAB5A|GAPDH|TFRC|CD63|Alix
Enriched markers
✔
PCNA|CANX|GAPDH|HSP90AA1|HSP90B1
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36706192    
Organism Homo sapiens
Experiment description Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles
Authors "Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q."
Journal name Sci Adv
Publication year 2023
Sample Embryonic kidney cells
Sample name HEK293T - Fraction 5
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density 1.151 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
20
Experiment ID 405
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|CD81|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Foreskin fibroblasts
Sample name BJ
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
21
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
22
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
23
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
24
Experiment ID 417
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX|ACTB
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Lymphoma cells
Sample name Raji
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
25
Experiment ID 411
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Mammary cancer-associated fibroblasts
Sample name mCAF
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
26
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
27
Experiment ID 418
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Monocytic leukemia cells
Sample name THP-1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
28
Experiment ID 418
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Monocytic leukemia cells
Sample name THP-1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
29
Experiment ID 413
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Normal mammary epithelial cells
Sample name MCF10A
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
30
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
31
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
32
Experiment ID 406
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35|CD81
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name BxPC3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
33
Experiment ID 415
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
34
Experiment ID 434
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
35
Experiment ID 435
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
36
Experiment ID 408
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|CD81|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX|ACTB
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic duct epithalial cells
Sample name HPDE
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
37
Experiment ID 409
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic duct epithalial cells
Sample name HPNE
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
38
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
39
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
40
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
41
Experiment ID 416
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pluripotent stem cells
Sample name PSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
42
Experiment ID 410
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample T lymphocytes
Sample name Jurkat
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
43
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for DNM1L
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CDCA5  
Affinity Capture-MS Homo sapiens
2 EIF3A 8661
Affinity Capture-MS Homo sapiens
3 GPM6A 2823
Affinity Capture-MS Homo sapiens
4 VPS35 55737
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
5 Kif19a  
Affinity Capture-MS Mus musculus
6 EIF2S1 1965
Affinity Capture-MS Homo sapiens
7 DPPA5  
Affinity Capture-MS Homo sapiens
8 MTCH1 23787
Proximity Label-MS Homo sapiens
9 MAGEA3  
Two-hybrid Homo sapiens
10 UBA1 7317
Affinity Capture-MS Homo sapiens
11 LDHB 3945
Affinity Capture-MS Homo sapiens
12 RPL30 6156
Affinity Capture-MS Homo sapiens
13 PSMA4 5685
Affinity Capture-MS Homo sapiens
14 LDHA 3939
Affinity Capture-MS Homo sapiens
15 SH3GL1 6455
Two-hybrid Homo sapiens
16 ATP6V1C1 528
Co-fractionation Homo sapiens
17 YWHAE 7531
Affinity Capture-MS Homo sapiens
18 Nek2  
Affinity Capture-MS Mus musculus
19 NACA 4666
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 KPNB1 3837
Affinity Capture-MS Homo sapiens
21 SUB1 10923
Affinity Capture-MS Homo sapiens
22 ACTC1 70
Proximity Label-MS Homo sapiens
23 DBN1 1627
Affinity Capture-MS Homo sapiens
24 MAGEA1  
Two-hybrid Homo sapiens
25 MTMR14 64419
Affinity Capture-MS Homo sapiens
26 HSPA4 3308
Affinity Capture-MS Homo sapiens
27 NTNG1  
Affinity Capture-MS Homo sapiens
28 SSB 6741
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
29 PPIA 5478
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 CALM1 801
Affinity Capture-MS Homo sapiens
31 LOC100132735  
Protein-RNA Homo sapiens
32 PTPN1 5770
Proximity Label-MS Homo sapiens
33 PARK7 11315
Affinity Capture-MS Homo sapiens
34 TRAF2 7186
Proximity Label-MS Homo sapiens
35 ILF2 3608
Affinity Capture-MS Homo sapiens
36 APEX1 328
Affinity Capture-RNA Homo sapiens
37 COPS5 10987
Affinity Capture-MS Homo sapiens
38 VPS26A 9559
Co-fractionation Homo sapiens
39 RUVBL1 8607
Affinity Capture-MS Homo sapiens
40 FIS1 51024
Affinity Capture-Western Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
41 RNPS1 10921
Affinity Capture-MS Homo sapiens
42 DARS 1615
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
43 RBMXL2  
Affinity Capture-MS Homo sapiens
44 TMEM183A  
Affinity Capture-MS Homo sapiens
45 CKMT1A 548596
Affinity Capture-MS Homo sapiens
46 TLN1 7094
Affinity Capture-MS Homo sapiens
47 NUFIP1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 EIF3B 8662
Affinity Capture-MS Homo sapiens
49 RAB3B 5865
Proximity Label-MS Homo sapiens
50 FBXW7  
Affinity Capture-MS Homo sapiens
51 IMPDH2 3615
Affinity Capture-MS Homo sapiens
52 IQGAP1 8826
Affinity Capture-MS Homo sapiens
53 TREML2 79865
Affinity Capture-MS Homo sapiens
54 ACTR1A 10121
Affinity Capture-MS Homo sapiens
55 HSPH1 10808
Affinity Capture-MS Homo sapiens
56 SAFB 6294
Affinity Capture-MS Homo sapiens
57 PSMC6 5706
Affinity Capture-MS Homo sapiens
58 MARCH5  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-Western Homo sapiens
59 JTB 10899
Affinity Capture-MS Homo sapiens
60 DNAJB1 3337
Co-fractionation Homo sapiens
61 ABCE1 6059
Affinity Capture-MS Homo sapiens
62 STIP1 10963
Affinity Capture-MS Homo sapiens
63 TBC1D15 64786
Co-fractionation Homo sapiens
64 EXD2  
Proximity Label-MS Homo sapiens
65 RCC1 1104
Affinity Capture-MS Homo sapiens
66 FIBP 9158
Affinity Capture-MS Homo sapiens
67 PDIA6 10130
Affinity Capture-MS Homo sapiens
68 SLC25A46 91137
Proximity Label-MS Homo sapiens
69 EEF1G 1937
Affinity Capture-MS Homo sapiens
70 DNAJC7 7266
Co-fractionation Homo sapiens
71 AASDHPPT 60496
Co-fractionation Homo sapiens
72 PGAM1 5223
Affinity Capture-MS Homo sapiens
73 CCT7 10574
Affinity Capture-MS Homo sapiens
74 MAVS 57506
Proximity Label-MS Homo sapiens
75 CA14 23632
Affinity Capture-MS Homo sapiens
76 WDR5 11091
Co-fractionation Homo sapiens
77 PLS3 5358
Affinity Capture-MS Homo sapiens
78 SOD1 6647
Affinity Capture-MS Homo sapiens
79 PSMC3 5702
Affinity Capture-MS Homo sapiens
80 TES 26136
Co-fractionation Homo sapiens
81 GSK3B 2932
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
82 DYNC1H1 1778
Affinity Capture-MS Homo sapiens
83 YWHAB 7529
Affinity Capture-MS Homo sapiens
84 CCT2 10576
Affinity Capture-MS Homo sapiens
85 NUP155 9631
Proximity Label-MS Homo sapiens
86 UNK  
Affinity Capture-RNA Homo sapiens
87 PXMP2  
Proximity Label-MS Homo sapiens
88 CHMP4C 92421
Affinity Capture-MS Homo sapiens
89 RPL27 6155
Affinity Capture-MS Homo sapiens
90 PRDX2 7001
Affinity Capture-MS Homo sapiens
91 CPNE2 221184
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
92 MTCH2 23788
Proximity Label-MS Homo sapiens
93 MAPK3 5595
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
94 SAMM50 25813
Proximity Label-MS Homo sapiens
95 DDX21 9188
Affinity Capture-MS Homo sapiens
96 RAB14 51552
Co-fractionation Homo sapiens
97 PARK2  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
98 SAE1 10055
Affinity Capture-MS Homo sapiens
99 SCG3  
Two-hybrid Homo sapiens
100 ZBTB24  
Reconstituted Complex Homo sapiens
101 HSP90B1 7184
Affinity Capture-MS Homo sapiens
102 HNRNPH3 3189
Affinity Capture-MS Homo sapiens
103 PSMC1 5700
Affinity Capture-MS Homo sapiens
104 ACIN1 22985
Affinity Capture-MS Homo sapiens
105 RAB4A 5867
Proximity Label-MS Homo sapiens
106 CAMK2G 818
Cross-Linking-MS (XL-MS) Homo sapiens
107 PFDN2 5202
Affinity Capture-MS Homo sapiens
108 PABPC1 26986
Affinity Capture-MS Homo sapiens
109 PSMD2 5708
Affinity Capture-MS Homo sapiens
110 VCP 7415
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
111 ACTN4 81
Affinity Capture-MS Homo sapiens
112 NTRK1 4914
Affinity Capture-MS Homo sapiens
113 GFAP 2670
Affinity Capture-MS Homo sapiens
114 TPI1 7167
Affinity Capture-MS Homo sapiens
115 CENPM  
Affinity Capture-MS Homo sapiens
116 HNRNPAB 3182
Affinity Capture-MS Homo sapiens
117 SUMO2 6613
Affinity Capture-MS Homo sapiens
118 DNAJA1 3301
Affinity Capture-MS Homo sapiens
119 PEX14 5195
Co-purification Homo sapiens
Proximity Label-MS Homo sapiens
120 SF3B6 51639
Affinity Capture-MS Homo sapiens
121 PSMC2 5701
Affinity Capture-MS Homo sapiens
122 EIF3D 8664
Affinity Capture-MS Homo sapiens
123 TGM1 7051
Affinity Capture-MS Homo sapiens
124 PRKAR2A 5576
Co-fractionation Homo sapiens
125 ACLY 47
Affinity Capture-MS Homo sapiens
126 DDX39B 7919
Affinity Capture-MS Homo sapiens
127 RHOT2 89941
Proximity Label-MS Homo sapiens
128 MYL12A 10627
Affinity Capture-MS Homo sapiens
129 DUSP23 54935
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
130 FZR1  
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
131 HIST1H2AB 8335
Affinity Capture-MS Homo sapiens
132 CARS 833
Co-fractionation Homo sapiens
133 RMDN3 55177
Proximity Label-MS Homo sapiens
134 CKMT1B 1159
Affinity Capture-MS Homo sapiens
135 EEF1D 1936
Affinity Capture-MS Homo sapiens
136 MAT2A 4144
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
137 PAICS 10606
Affinity Capture-MS Homo sapiens
138 HNRNPDL 9987
Affinity Capture-MS Homo sapiens
139 ACTN1 87
Affinity Capture-MS Homo sapiens
140 DNAAF2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
141 PRDX6 9588
Affinity Capture-MS Homo sapiens
142 FGL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
143 CYB5B 80777
Affinity Capture-MS Homo sapiens
144 EFNA4  
Affinity Capture-MS Homo sapiens
145 FAM129B 64855
Co-fractionation Homo sapiens
146 AHCY 191
Affinity Capture-MS Homo sapiens
147 UBE2H 7328
Affinity Capture-MS Homo sapiens
148 RAB5A 5868
Proximity Label-MS Homo sapiens
149 GANAB 23193
Affinity Capture-MS Homo sapiens
150 Wdr5  
Affinity Capture-MS Mus musculus
151 PNN 5411
Affinity Capture-MS Homo sapiens
152 MTX2 10651
Proximity Label-MS Homo sapiens
153 UBE2L3 7332
Affinity Capture-MS Homo sapiens
154 EEF2 1938
Affinity Capture-MS Homo sapiens
155 KCTD12 115207
Affinity Capture-MS Homo sapiens
156 HSD3B7 80270
Proximity Label-MS Homo sapiens
157 YWHAZ 7534
Affinity Capture-MS Homo sapiens
158 EGFR 1956
Affinity Capture-MS Homo sapiens
Negative Genetic Homo sapiens
159 FKBP8 23770
Proximity Label-MS Homo sapiens
160 HNRNPA1L2 144983
Affinity Capture-MS Homo sapiens
161 NAP1L1 4673
Affinity Capture-MS Homo sapiens
162 EEF1B2 1933
Affinity Capture-MS Homo sapiens
163 ERH 2079
Affinity Capture-MS Homo sapiens
164 HSD17B10 3028
Affinity Capture-MS Homo sapiens
165 LARS 51520
Affinity Capture-MS Homo sapiens
166 ARFIP1 27236
Co-fractionation Homo sapiens
167 YWHAQ 10971
Affinity Capture-MS Homo sapiens
168 DDX19B 11269
Proximity Label-MS Homo sapiens
169 RRM2 6241
Two-hybrid Homo sapiens
170 TPM1 7168
Affinity Capture-MS Homo sapiens
171 MUL1  
Biochemical Activity Homo sapiens
172 RPA3 6119
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
173 NME1 4830
Affinity Capture-MS Homo sapiens
174 APEH 327
Co-fractionation Homo sapiens
175 RAB9A 9367
Proximity Label-MS Homo sapiens
176 SEC24A 10802
Co-fractionation Homo sapiens
177 MTHFD1 4522
Affinity Capture-MS Homo sapiens
178 RPL12 6136
Affinity Capture-MS Homo sapiens
179 HPRT1 3251
Affinity Capture-MS Homo sapiens
180 PSMC5 5705
Affinity Capture-MS Homo sapiens
181 SH3GLB2 56904
Proximity Label-MS Homo sapiens
182 MFN2 9927
Proximity Label-MS Homo sapiens
183 RANBP1 5902
Affinity Capture-MS Homo sapiens
184 CALM3 808
Affinity Capture-MS Homo sapiens
185 CAMK2D 817
Cross-Linking-MS (XL-MS) Homo sapiens
186 HAX1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
187 AKT1 207
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
188 THADA 63892
Proximity Label-MS Homo sapiens
189 PEX3 8504
Proximity Label-MS Homo sapiens
190 AKAP1 8165
Proximity Label-MS Homo sapiens
191 XRCC5 7520
Affinity Capture-MS Homo sapiens
192 FAM133A 286499
Affinity Capture-MS Homo sapiens
193 ATIC 471
Co-fractionation Homo sapiens
194 MYH11 4629
Affinity Capture-MS Homo sapiens
195 RRM1 6240
Co-fractionation Homo sapiens
196 MIEF1  
Affinity Capture-Western Homo sapiens
197 AHSA1 10598
Affinity Capture-MS Homo sapiens
198 GYPA  
Affinity Capture-MS Homo sapiens
199 LLGL2 3993
Affinity Capture-MS Homo sapiens
200 EIF3F 8665
Affinity Capture-MS Homo sapiens
201 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
202 FASN 2194
Negative Genetic Homo sapiens
Affinity Capture-MS Homo sapiens
203 OCIAD1 54940
Proximity Label-MS Homo sapiens
204 PEX11B 8799
Phenotypic Enhancement Homo sapiens
205 CCT8 10694
Affinity Capture-MS Homo sapiens
206 TMEM9 252839
Affinity Capture-MS Homo sapiens
207 PARP1 142
Proximity Label-MS Homo sapiens
208 RUVBL2 10856
Affinity Capture-MS Homo sapiens
209 XRCC6 2547
Affinity Capture-MS Homo sapiens
210 CKB 1152
Affinity Capture-MS Homo sapiens
211 EZR 7430
Affinity Capture-MS Homo sapiens
212 SAP18 10284
Affinity Capture-MS Homo sapiens
213 CDC37 11140
Affinity Capture-MS Homo sapiens
214 EDEM1  
Affinity Capture-MS Homo sapiens
215 SKP1 6500
Affinity Capture-MS Homo sapiens
216 PTBP1 5725
Affinity Capture-MS Homo sapiens
217 NHLRC2 374354
Affinity Capture-MS Homo sapiens
218 DCTN2 10540
Affinity Capture-MS Homo sapiens
219 PDHB 5162
Affinity Capture-MS Homo sapiens
220 MDH2 4191
Affinity Capture-MS Homo sapiens
221 CAPRIN1 4076
Affinity Capture-MS Homo sapiens
222 TKT 7086
Affinity Capture-MS Homo sapiens
223 SERPINB2 5055
Co-fractionation Homo sapiens
224 EIF4A3 9775
Affinity Capture-MS Homo sapiens
225 MAPK1 5594
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
226 BUB3 9184
Affinity Capture-MS Homo sapiens
227 CDK1 983
Affinity Capture-MS Homo sapiens
228 WIF1 11197
Affinity Capture-MS Homo sapiens
229 PEX11G  
Phenotypic Enhancement Homo sapiens
230 DCD 117159
Affinity Capture-MS Homo sapiens
231 MFF 56947
Proximity Label-MS Homo sapiens
232 PFN1 5216
Affinity Capture-MS Homo sapiens
233 TXN 7295
Affinity Capture-MS Homo sapiens
234 PEX11A  
Phenotypic Enhancement Homo sapiens
235 CALM2 805
Affinity Capture-MS Homo sapiens
236 GABRR1  
Affinity Capture-MS Homo sapiens
237 TAGLN2 8407
Affinity Capture-MS Homo sapiens
238 LRRK2 120892
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
239 PSMG3 84262
Co-fractionation Homo sapiens
240 CYHR1  
Co-fractionation Homo sapiens
241 ITPA 3704
Co-fractionation Homo sapiens
242 TRIM28 10155
Affinity Capture-MS Homo sapiens
243 RBM8A 9939
Affinity Capture-MS Homo sapiens
244 DGUOK  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
245 ARF1 375
Affinity Capture-MS Homo sapiens
246 C14orf166 51637
Affinity Capture-MS Homo sapiens
247 RPL28 6158
Affinity Capture-MS Homo sapiens
248 SERBP1 26135
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
249 EIF5A 1984
Affinity Capture-MS Homo sapiens
250 SNW1 22938
Affinity Capture-MS Homo sapiens
251 KRAS 3845
Synthetic Lethality Homo sapiens
252 RARS 5917
Affinity Capture-MS Homo sapiens
253 LRRC49  
Proximity Label-MS Homo sapiens
254 PSMC4 5704
Affinity Capture-MS Homo sapiens
255 RBBP4 5928
Affinity Capture-MS Homo sapiens
256 PRMT1 3276
Affinity Capture-MS Homo sapiens
257 PCMT1 5110
Affinity Capture-MS Homo sapiens
258 JMJD6 23210
Co-fractionation Homo sapiens
259 HIST1H2AE 3012
Affinity Capture-MS Homo sapiens
260 TIMM13 26517
Affinity Capture-MS Homo sapiens
261 CUL4A 8451
Affinity Capture-MS Homo sapiens
262 ALDOA 226
Affinity Capture-MS Homo sapiens
263 CDC5L 988
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which DNM1L is involved
PathwayEvidenceSource
Apoptosis TAS Reactome
Apoptotic execution phase TAS Reactome
Programmed Cell Death TAS Reactome





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