Gene description for CHD1L
Gene name chromodomain helicase DNA binding protein 1-like
Gene symbol CHD1L
Other names/aliases ALC1
CHDL
Species Homo sapiens
 Database cross references - CHD1L
ExoCarta ExoCarta_9557
Vesiclepedia VP_9557
Entrez Gene 9557
HGNC 1916
MIM 613039
UniProt Q86WJ1  
 CHD1L identified in sEVs derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Hepatocytes 26054723    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Thymus 23844026    
 Gene ontology annotations for CHD1L
Molecular Function
    nucleotide binding GO:0000166 IBA
    nucleotide binding GO:0000166 IDA
    DNA helicase activity GO:0003678 TAS
    DNA clamp loader activity GO:0003689 IEA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    four-way junction helicase activity GO:0009378 IEA
    ATP hydrolysis activity GO:0016887 IMP
    nucleosome binding GO:0031491 IDA
    double-stranded DNA helicase activity GO:0036121 IEA
    forked DNA-dependent helicase activity GO:0061749 IEA
    cohesin loader activity GO:0061775 IEA
    histone reader activity GO:0140566 IDA
    chromatin extrusion motor activity GO:0140584 IEA
    ATP-dependent chromatin remodeler activity GO:0140658 IDA
    ATP-dependent H3-H4 histone complex chaperone activity GO:0140665 IEA
    ATP-dependent H2AZ histone chaperone activity GO:0140849 IEA
    poly-ADP-D-ribose modification-dependent protein binding GO:0160004 IDA
    single-stranded 3'-5' DNA helicase activity GO:1990518 IEA
Biological Process
    DNA repair GO:0006281 IDA
    DNA repair GO:0006281 TAS
    chromatin remodeling GO:0006338 IBA
    chromatin remodeling GO:0006338 IDA
    chromatin remodeling GO:0006338 IEA
    DNA damage response GO:0006974 IBA
    DNA damage response GO:0006974 IDA
    DNA duplex unwinding GO:0032508 IEA
    chromatin looping GO:0140588 IEA
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    cytosol GO:0005829 IDA
    plasma membrane GO:0005886 IDA
    site of double-strand break GO:0035861 IDA
    site of DNA damage GO:0090734 IDA
 Experiment description of studies that identified CHD1L in sEVs
1
Experiment ID 236
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
3
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
5
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for CHD1L
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TGOLN2 10618
Affinity Capture-MS Homo sapiens
2 HLTF  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 HIST2H2BE 8349
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 FAM196A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 NT5E 4907
Affinity Capture-MS Homo sapiens
6 CCL22 6367
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 EIF4A1 1973
Affinity Capture-MS Homo sapiens
8 CBX1 10951
Affinity Capture-MS Homo sapiens
9 ART3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 PARP1 142
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Synthetic Rescue Homo sapiens
11 FAS 355
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 GPR17 2840
Affinity Capture-MS Homo sapiens
13 Osgep  
Affinity Capture-MS Mus musculus
14 CIAO1 9391
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
15 TRIM33 51592
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Co-localization Homo sapiens
16 KLK5 25818
Affinity Capture-MS Homo sapiens
17 H3F3AP6  
Affinity Capture-MS Homo sapiens
18 KLK11 11012
Affinity Capture-MS Homo sapiens
19 FOXP1 27086
Affinity Capture-MS Homo sapiens
20 FOXB1  
Affinity Capture-MS Homo sapiens
21 GALNT10 55568
Affinity Capture-MS Homo sapiens
22 RPA1 6117
Affinity Capture-MS Homo sapiens
23 ID1  
Affinity Capture-MS Homo sapiens
24 TNKS2 80351
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 GMDS 2762
Affinity Capture-MS Homo sapiens
26 SGOL1  
Affinity Capture-MS Homo sapiens
27 HIST1H2BB 3018
Affinity Capture-MS Homo sapiens
28 CBX3 11335
Affinity Capture-MS Homo sapiens
29 LAMP2 3920
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 RPA2 6118
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 HSPA8 3312
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 PARK2  
Affinity Capture-MS Homo sapiens
33 NDUFA4 4697
Affinity Capture-MS Homo sapiens
34 FOXL1  
Affinity Capture-MS Homo sapiens
35 METTL21B  
Affinity Capture-MS Homo sapiens
36 PLK1 5347
Affinity Capture-MS Homo sapiens
37 XPC  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 SCYL1 57410
Two-hybrid Homo sapiens
39 PRKDC 5591
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
40 SDC2 6383
Affinity Capture-MS Homo sapiens
41 PARP2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
43 TNKS 8658
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 RNF146  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 KIR2DS2  
Affinity Capture-MS Homo sapiens
46 XRCC1 7515
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 NEIL1  
Affinity Capture-MS Homo sapiens
48 HIST1H2AM 8336
Affinity Capture-MS Homo sapiens
49 HIST2H2AA4 723790
Affinity Capture-MS Homo sapiens
50 RFC2 5982
Affinity Capture-MS Homo sapiens
51 BTF3 689
Affinity Capture-MS Homo sapiens
52 LRRK2 120892
Affinity Capture-MS Homo sapiens
53 HIST1H2BA 255626
Affinity Capture-MS Homo sapiens
54 HIST3H2A 92815
Affinity Capture-MS Homo sapiens
55 NTRK1 4914
Affinity Capture-MS Homo sapiens
56 RNF166  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 CBX5 23468
Affinity Capture-MS Homo sapiens
58 FAM134A 79137
Affinity Capture-MS Homo sapiens
59 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
60 TEAD2  
Affinity Capture-MS Homo sapiens
61 API5 8539
Affinity Capture-MS Homo sapiens
62 XRCC6 2547
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
63 GLMP 112770
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
64 SNW1 22938
Affinity Capture-MS Homo sapiens
65 C9orf72  
Affinity Capture-MS Homo sapiens
66 FAM174A 345757
Affinity Capture-MS Homo sapiens
67 KLRC1  
Affinity Capture-MS Homo sapiens
68 HIST1H2BK 85236
Affinity Capture-MS Homo sapiens
69 H2AFZ 3015
Affinity Capture-MS Homo sapiens
70 SSRP1 6749
Affinity Capture-MS Homo sapiens
71 FAM35A  
Proximity Label-MS Homo sapiens
72 RPA3 6119
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
73 APLF  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
74 SUPT16H 11198
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
75 NR4A1  
Two-hybrid Homo sapiens
76 XRCC5 7520
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
77 CDC5L 988
Affinity Capture-MS Homo sapiens
78 H2AFX 3014
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
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