Gene description for CPSF1
Gene name cleavage and polyadenylation specific factor 1, 160kDa
Gene symbol CPSF1
Other names/aliases CPSF160
HSU37012
P/cl.18
Species Homo sapiens
 Database cross references - CPSF1
ExoCarta ExoCarta_29894
Entrez Gene 29894
HGNC 2324
MIM 606027
UniProt Q10570  
 CPSF1 identified in exosomes derived from the following tissue/cell type
Hepatocytes 26054723    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Thymus 23844026    
 Gene ontology annotations for CPSF1
Molecular Function
    mRNA 3'-UTR binding GO:0003730 IDA
    protein binding GO:0005515 IPI
Biological Process
    mRNA cleavage GO:0006379 IDA
    termination of RNA polymerase II transcription GO:0006369 TAS
    mRNA polyadenylation GO:0006378 IDA
    mRNA 3'-end processing GO:0031124 TAS
    mRNA splicing, via spliceosome GO:0000398 TAS
    gene expression GO:0010467 TAS
    RNA splicing GO:0008380 TAS
    transcription from RNA polymerase II promoter GO:0006366 TAS
    mRNA export from nucleus GO:0006406 TAS
Subcellular Localization
    nucleoplasm GO:0005654 TAS
    mRNA cleavage and polyadenylation specificity factor complex GO:0005847 IDA
 Experiment description of studies that identified CPSF1 in exosomes
1
Experiment ID 237
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
2
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 217
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
CD81|CD9|CD63
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for CPSF1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TAF5  
Invitro Homo sapiens
2 TAF7  
Invitro Homo sapiens
3 TAF11  
Invitro Homo sapiens
4 PRRC2A 7916
Two-hybrid Homo sapiens
5 CSTF2  
Affinity Capture-MS Homo sapiens
6 TAF12  
Invitro Homo sapiens
7 MCM2 4171
Invitro Homo sapiens
8 TAF13  
Invitro Homo sapiens
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