Gene description for APEX1
Gene name APEX nuclease (multifunctional DNA repair enzyme) 1
Gene symbol APEX1
Other names/aliases APE
APE1
APEN
APEX
APX
HAP1
REF1
Species Homo sapiens
 Database cross references - APEX1
ExoCarta ExoCarta_328
Entrez Gene 328
HGNC 587
MIM 107748
UniProt P27695  
 APEX1 identified in exosomes derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for APEX1
Molecular Function
    phosphoric diester hydrolase activity GO:0008081 IDA
    uracil DNA N-glycosylase activity GO:0004844 TAS
    site-specific endodeoxyribonuclease activity, specific for altered base GO:0016890 IDA
    poly(A) RNA binding GO:0044822 IDA
    DNA-(apurinic or apyrimidinic site) lyase activity GO:0003906 TAS
    endonuclease activity GO:0004519 IDA
    RNA-DNA hybrid ribonuclease activity GO:0004523 TAS
    NF-kappaB binding GO:0051059 IEA
    DNA binding GO:0003677 IDA
    protein complex binding GO:0032403 IEA
    transcription coactivator activity GO:0003713 IDA
    phosphodiesterase I activity GO:0004528 TAS
    oxidoreductase activity GO:0016491 IDA
    chromatin DNA binding GO:0031490 IDA
    endodeoxyribonuclease activity GO:0004520 TAS
    protein binding GO:0005515 IPI
    3'-5' exonuclease activity GO:0008408 TAS
    metal ion binding GO:0046872 IDA
    damaged DNA binding GO:0003684 IDA
    double-stranded DNA 3'-5' exodeoxyribonuclease activity GO:0008311 IBA
    transcription corepressor activity GO:0003714 TAS
Biological Process
    transcription, DNA-templated GO:0006351 IEA
    mitotic cell cycle GO:0000278 TAS
    positive regulation of DNA repair GO:0045739 IDA
    transcription-coupled nucleotide-excision repair GO:0006283 TAS
    DNA strand elongation involved in DNA replication GO:0006271 TAS
    nucleotide-excision repair GO:0006289 TAS
    negative regulation of nucleic acid-templated transcription GO:1903507 TAS
    DNA repair GO:0006281 TAS
    regulation of mRNA stability GO:0043488 IMP
    telomere maintenance GO:0000723 TAS
    double-strand break repair via homologous recombination GO:0000724 TAS
    telomere maintenance via semi-conservative replication GO:0032201 TAS
    cell redox homeostasis GO:0045454 IEA
    cellular response to cAMP GO:0071320 IEA
    oxidation-reduction process GO:0055114 IDA
    regulation of transcription, DNA-templated GO:0006355 IEA
    response to drug GO:0042493 IEA
    cellular response to peptide hormone stimulus GO:0071375 IEA
    positive regulation of G1/S transition of mitotic cell cycle GO:1900087 IEA
    base-excision repair GO:0006284 TAS
    nucleotide-excision repair, DNA gap filling GO:0006297 TAS
    telomere maintenance via recombination GO:0000722 TAS
    aging GO:0007568 IEA
    negative regulation of smooth muscle cell migration GO:0014912 IEA
    RNA phosphodiester bond hydrolysis, endonucleolytic GO:0090502 IEA
    DNA demethylation GO:0080111 IDA
    cellular response to hydrogen peroxide GO:0070301 IEA
    mismatch repair GO:0006298 TAS
    double-strand break repair GO:0006302 TAS
Subcellular Localization
    transcription factor complex GO:0005667 IEA
    nucleoplasm GO:0005654 TAS
    perinuclear region of cytoplasm GO:0048471 IDA
    mitochondrion GO:0005739 IDA
    nucleolus GO:0005730 IDA
    cytoplasm GO:0005737 IDA
    ribosome GO:0005840 TAS
    nuclear speck GO:0016607 IDA
    nucleus GO:0005634 IDA
    endoplasmic reticulum GO:0005783 TAS
    centrosome GO:0005813 IDA
 Experiment description of studies that identified APEX1 in exosomes
1
Experiment ID 211
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
cytochrome c|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
Cytochrome C|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for APEX1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 DCTN1 1639
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
2 XRCC6 2547
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
3 MUTYH  
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
4 HIF1A  
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
5 UBE2I 7329
Invitro Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
6 TXN 7295
Invitro Homo sapiens
7 XRCC1  
Invivo Homo sapiens
Two-hybrid Homo sapiens
8 HMGB2 3148
Affinity Capture-MS Homo sapiens
9 HNRNPL 3191
Invivo Homo sapiens
Invitro Homo sapiens
10 NME1 4830
Affinity Capture-MS Homo sapiens
11 ANP32A 8125
Affinity Capture-Western Homo sapiens
12 SET 6418
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
13 PCNA 5111
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
14 HSPA1A  
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
15 TP53  
Co-purification Homo sapiens
Affinity Capture-MS Homo sapiens
16 FEN1 2237
Invitro Homo sapiens
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
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