Gene description for CCNA2
Gene name cyclin A2
Gene symbol CCNA2
Other names/aliases CCN1
CCNA
Species Homo sapiens
 Database cross references - CCNA2
ExoCarta ExoCarta_890
Entrez Gene 890
HGNC 1578
MIM 123835
UniProt P20248  
 CCNA2 identified in exosomes derived from the following tissue/cell type
Endothelial cells 26027894    
 Gene ontology annotations for CCNA2
Molecular Function
    protein binding GO:0005515 IPI
    protein kinase binding GO:0019901 IEA
Biological Process
    regulation of cyclin-dependent protein serine/threonine kinase activity GO:0000079 IEA
    cell division GO:0051301 IEA
    G2/M transition of mitotic cell cycle GO:0000086 TAS
    viral process GO:0016032 IEA
    positive regulation of transcription, DNA-templated GO:0045893 IEA
    mitotic nuclear division GO:0007067 IEA
    response to glucagon GO:0033762 IEA
    positive regulation of fibroblast proliferation GO:0048146 IEA
    mitotic cell cycle GO:0000278 TAS
    response to estradiol GO:0032355 IEA
    organ regeneration GO:0031100 IEA
    Ras protein signal transduction GO:0007265 IEP
    regulation of G2/M transition of mitotic cell cycle GO:0010389 IEA
    mitotic G2 DNA damage checkpoint GO:0007095 TAS
Subcellular Localization
    male pronucleus GO:0001940 IEA
    female pronucleus GO:0001939 IEA
    nucleoplasm GO:0005654 TAS
    nucleus GO:0005634 IDA
    cytoplasm GO:0005737 IDA
 Experiment description of studies that identified CCNA2 in exosomes
1
Experiment ID 226
ISEV standards
EM
EV Biophysical techniques
GAPDH
EV Cytosolic markers
CD9|FLOT1
EV Membrane markers
LMNA|H2AFX|ATP5A1|TOMM20
EV Negative markers
EV Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting
Authors Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
 Protein-protein interactions for CCNA2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CDC20  
Invivo Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
2 RBL1  
Affinity Capture-Western Homo sapiens
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
3 PTMA 5757
Invitro Homo sapiens
4 FEN1 2237
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
5 E2F1 1869
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Invitro Homo sapiens
6 BRCA1 672
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-Western Homo sapiens
7 SKP1 6500
Invivo Homo sapiens
8 SKP2  
Affinity Capture-Western Homo sapiens
Invivo Homo sapiens
9 CDC6  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
10 CALM1 801
Reconstituted Complex Homo sapiens
11 CDKN1A  
Affinity Capture-Western Homo sapiens
12 CDK2 1017
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-Western Homo sapiens
13 TAF1  
Invitro Homo sapiens
Invivo Homo sapiens
14 CKS1B  
Affinity Capture-Western Homo sapiens
15 CDK1 983
Invivo Homo sapiens
Affinity Capture-Western Homo sapiens
16 PCNA 5111
Affinity Capture-Western Homo sapiens
17 ITGB3BP  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
18 CDC25C  
Invitro Homo sapiens
19 RBL2 5934
Affinity Capture-Western Homo sapiens
20 TP53  
Reconstituted Complex Homo sapiens
21 MYBL2 4605
Invivo Homo sapiens
22 SIC1  
Reconstituted Complex 4932
Biochemical Activity 4932
23 CDKN1B 1027
Invitro Homo sapiens
Affinity Capture-Western Homo sapiens
24 CDKN1C  
Affinity Capture-Western Homo sapiens
25 HERC5 51191
Reconstituted Complex Homo sapiens
View the network image/svg+xml



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