Gene description for TK1
Gene name thymidine kinase 1, soluble
Gene symbol TK1
Other names/aliases TK2
Species Homo sapiens
 Database cross references - TK1
ExoCarta ExoCarta_7083
Entrez Gene 7083
HGNC 11830
MIM 188300
UniProt P04183  
 TK1 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for TK1
Molecular Function
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    identical protein binding GO:0042802 IPI
    thymidine kinase activity GO:0004797 IEA
    nucleoside kinase activity GO:0019206 EXP
    zinc ion binding GO:0008270 IDA
Biological Process
    phosphorylation GO:0016310 IEA
    skeletal muscle cell proliferation GO:0014856 IEA
    response to toxic substance GO:0009636 IEA
    small molecule metabolic process GO:0044281 TAS
    DNA biosynthetic process GO:0071897 IEA
    response to nutrient levels GO:0031667 IEA
    nucleobase-containing small molecule metabolic process GO:0055086 TAS
    nucleobase-containing compound metabolic process GO:0006139 TAS
    pyrimidine nucleobase metabolic process GO:0006206 TAS
    protein homotetramerization GO:0051289 IPI
    nucleotide biosynthetic process GO:0009165 EXP
    deoxyribonucleoside monophosphate biosynthetic process GO:0009157 TAS
    thymidine metabolic process GO:0046104 IEA
    digestive tract development GO:0048565 IEA
    response to cortisol GO:0051414 IEA
    response to copper ion GO:0046688 IEA
    liver development GO:0001889 IEA
    fetal process involved in parturition GO:0060138 IEA
    pyrimidine nucleoside salvage GO:0043097 TAS
Subcellular Localization
    cytosol GO:0005829 TAS
 Experiment description of studies that identified TK1 in exosomes
Experiment ID 235
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for TK1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 DKFZP564O0523  
Two-hybrid Homo sapiens
2 GAPDH 2597
Two-hybrid Homo sapiens
3 PTPRK 5796
Two-hybrid Homo sapiens
4 UNC119  
Two-hybrid Homo sapiens
5 MDS025  
Two-hybrid Homo sapiens
6 GDF9 2661
Two-hybrid Homo sapiens
7 DRAP1  
Two-hybrid Homo sapiens
8 APLP1  
Two-hybrid Homo sapiens
9 TP53  
Two-hybrid Homo sapiens
10 TUBB2A 7280
Two-hybrid Homo sapiens
11 TLE1  
Two-hybrid Homo sapiens
12 CDKN1A  
Affinity Capture-MS Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
13 C14orf1 11161
Two-hybrid Homo sapiens
14 COPS6 10980
Two-hybrid Homo sapiens
15 CRMP1 1400
Two-hybrid Homo sapiens
16 SETDB1  
Two-hybrid Homo sapiens
17 RIF1  
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which TK1 is involved
Pyrimidine salvage reactions TAS Reactome

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