Gene description for FYCO1
Gene name FYVE and coiled-coil domain containing 1
Gene symbol FYCO1
Other names/aliases CATC2
CTRCT18
RUFY3
ZFYVE7
Species Homo sapiens
 Database cross references - FYCO1
ExoCarta ExoCarta_79443
Vesiclepedia VP_79443
Entrez Gene 79443
HGNC 14673
MIM 607182
UniProt Q9BQS8  
 FYCO1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Hepatocytes 26054723    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for FYCO1
Molecular Function
    protein binding GO:0005515 IPI
    metal ion binding GO:0046872 IEA
Biological Process
    plus-end-directed vesicle transport along microtubule GO:0072383 IBA
    plus-end-directed vesicle transport along microtubule GO:0072383 IMP
    positive regulation of autophagosome maturation GO:1901098 IBA
    positive regulation of autophagosome maturation GO:1901098 IMP
Subcellular Localization
    lysosome GO:0005764 IBA
    lysosome GO:0005764 IDA
    late endosome GO:0005770 IBA
    late endosome GO:0005770 IDA
    autophagosome GO:0005776 IBA
    autophagosome GO:0005776 IDA
    Golgi apparatus GO:0005794 IDA
    membrane GO:0016020 HDA
    intracellular membrane-bounded organelle GO:0043231 IDA
 Experiment description of studies that identified FYCO1 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 207
MISEV standards
EM
Biophysical techniques
TSG101|HSP70|FLOT1
Enriched markers
VDAC
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 208
MISEV standards
EM
Biophysical techniques
TSG101|HSP70|FLOT1
Enriched markers
VDAC
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 209
MISEV standards
Biophysical techniques
TSG101|HSP70|FLOT1
Enriched markers
VDAC
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 237
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
9
Experiment ID 488
MISEV standards
EM
Biophysical techniques
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for FYCO1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 KRT72 140807
Affinity Capture-MS Homo sapiens
2 SEC24B 10427
Affinity Capture-MS Homo sapiens
3 DDX60 55601
Affinity Capture-MS Homo sapiens
4 MAP1LC3C  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
5 ABCF3 55324
Affinity Capture-MS Homo sapiens
6 C18orf8 29919
Affinity Capture-MS Homo sapiens
7 PAX6  
Affinity Capture-MS Homo sapiens
8 CCAR1 55749
Affinity Capture-MS Homo sapiens
9 IQSEC1 9922
Affinity Capture-MS Homo sapiens
10 CCDC68  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 SIRT6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 CCT3 7203
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 SLU7  
Affinity Capture-MS Homo sapiens
14 PRMT6  
Two-hybrid Homo sapiens
15 SLC35G1  
Affinity Capture-MS Homo sapiens
16 LAMTOR1 55004
Proximity Label-MS Homo sapiens
17 XPO1 7514
Affinity Capture-MS Homo sapiens
18 RBM25 58517
Co-fractionation Homo sapiens
19 CCT2 10576
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 KRT86  
Affinity Capture-MS Homo sapiens
21 MON1B 22879
Affinity Capture-MS Homo sapiens
22 RAMP1  
Affinity Capture-MS Homo sapiens
23 RPA2 6118
Affinity Capture-MS Homo sapiens
24 IQCB1  
Affinity Capture-MS Homo sapiens
25 TRUB2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 TCP1 6950
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 TCP10L  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 DTNA  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 MAP4 4134
Affinity Capture-MS Homo sapiens
30 TCF7L2  
Affinity Capture-MS Homo sapiens
31 EMD 2010
Affinity Capture-MS Homo sapiens
32 LAMP2 3920
Proximity Label-MS Homo sapiens
33 PARP14 54625
Affinity Capture-MS Homo sapiens
34 RCCD1  
Affinity Capture-MS Homo sapiens
35 KLHDC2  
Affinity Capture-MS Homo sapiens
36 HAUS5 23354
Affinity Capture-MS Homo sapiens
37 SEC23B 10483
Affinity Capture-MS Homo sapiens
38 CCT6A 908
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 KIF5B 3799
Affinity Capture-MS Homo sapiens
40 CCDC71  
Affinity Capture-MS Homo sapiens
41 APP 351
Reconstituted Complex Homo sapiens
42 CCT7 10574
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 TMPO 7112
Affinity Capture-MS Homo sapiens
44 MAP1LC3B 81631
Affinity Capture-MS Homo sapiens
Protein-peptide Homo sapiens
Affinity Capture-MS Homo sapiens
45 ADO  
Affinity Capture-MS Homo sapiens
46 MAP1LC3A 84557
Affinity Capture-MS Homo sapiens
Protein-peptide Homo sapiens
Affinity Capture-MS Homo sapiens
47 PPL 5493
Affinity Capture-MS Homo sapiens
48 HIF1AN 55662
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 KDM1A 23028
Two-hybrid Homo sapiens
50 CTCFL  
Affinity Capture-MS Homo sapiens
51 POLDIP3 84271
Affinity Capture-MS Homo sapiens
52 MOV10 4343
Affinity Capture-RNA Homo sapiens
53 RFT1 91869
Affinity Capture-MS Homo sapiens
54 KRT38 8687
Affinity Capture-MS Homo sapiens
55 RPA3 6119
Affinity Capture-MS Homo sapiens
56 DTNB 1838
Affinity Capture-MS Homo sapiens
57 KIF23 9493
Affinity Capture-MS Homo sapiens
58 RAB7A 7879
Proximity Label-MS Homo sapiens
59 RAB9A 9367
Proximity Label-MS Homo sapiens
60 KXD1 79036
Affinity Capture-MS Homo sapiens
61 EP300 2033
Affinity Capture-MS Homo sapiens
62 CADPS  
Affinity Capture-MS Homo sapiens
63 STK11 6794
Affinity Capture-MS Homo sapiens
64 RNF145  
Affinity Capture-MS Homo sapiens
65 RAF1 5894
Two-hybrid Homo sapiens
66 PIGF  
Affinity Capture-MS Homo sapiens
67 CCDC101  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
68 CCT6B 10693
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
69 RPA1 6117
Affinity Capture-MS Homo sapiens
70 CCZ1 51622
Affinity Capture-MS Homo sapiens
71 EXPH5  
Affinity Capture-MS Homo sapiens
72 GOPC 57120
Affinity Capture-MS Homo sapiens
73 LMNA 4000
Affinity Capture-MS Homo sapiens
74 FOXK1 221937
Affinity Capture-MS Homo sapiens
75 NOL9 79707
Affinity Capture-MS Homo sapiens
76 FTSJ3 117246
Affinity Capture-MS Homo sapiens
77 SASS6 163786
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
78 CYLD  
Affinity Capture-MS Homo sapiens
79 C17orf59 54785
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
80 FAM9C  
Affinity Capture-MS Homo sapiens
81 MLYCD 23417
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
82 ZBTB44  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which FYCO1 is involved
No pathways found





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