Gene description for POLR1B
Gene name polymerase (RNA) I polypeptide B, 128kDa
Gene symbol POLR1B
Other names/aliases RPA135
RPA2
Rpo1-2
Species Homo sapiens
 Database cross references - POLR1B
ExoCarta ExoCarta_84172
Vesiclepedia VP_84172
Entrez Gene 84172
HGNC 20454
MIM 602000
UniProt Q9H9Y6  
 POLR1B identified in exosomes derived from the following tissue/cell type
Chondrocytes 35931686    
Chondrocytes 35931686    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for POLR1B
Molecular Function
    RNA polymerase I activity GO:0001054 IBA
    RNA polymerase II activity GO:0001055 IEA
    RNA polymerase III activity GO:0001056 IEA
    RNA polymerase IV activity GO:0001057 IEA
    RNA polymerase V activity GO:0001058 IEA
    mitochondrial single subunit type RNA polymerase activity GO:0001065 IEA
    plastid single subunit type RNA polymerase activity GO:0001066 IEA
    DNA binding GO:0003677 IEA
    DNA-directed 5'-3' RNA polymerase activity GO:0003899 IDA
    protein binding GO:0005515 IPI
    zinc ion binding GO:0008270 IDA
    ribonucleoside binding GO:0032549 IEA
    DNA/RNA hybrid binding GO:0071667 IDA
Biological Process
    transcription by RNA polymerase IV GO:0001059 IEA
    transcription by RNA polymerase V GO:0001060 IEA
    transcription by RNA polymerase I GO:0006360 IEA
    transcription by RNA polymerase II GO:0006366 IEA
    transcription by RNA polymerase III GO:0006383 IEA
    mitochondrial transcription GO:0006390 IEA
    embryo implantation GO:0007566 IEA
    rRNA transcription GO:0009303 IEA
    neural crest formation GO:0014029 ISS
    nucleologenesis GO:0017126 IEA
    plastid transcription GO:0042793 IEA
Subcellular Localization
    fibrillar center GO:0001650 IDA
    nucleoplasm GO:0005654 TAS
    chromosome GO:0005694 ISS
    RNA polymerase I complex GO:0005736 IBA
    RNA polymerase I complex GO:0005736 IDA
    cytosol GO:0005829 IDA
 Experiment description of studies that identified POLR1B in exosomes
1
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for POLR1B
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HNRNPH1 3187
Affinity Capture-MS Homo sapiens
2 POLR2G 5436
Affinity Capture-MS Homo sapiens
3 RIT1 6016
Negative Genetic Homo sapiens
4 MCM7 4176
Co-fractionation Homo sapiens
5 CD3EAP  
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
6 RRN3  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
7 KPNA1 3836
Affinity Capture-MS Homo sapiens
8 EIF2S3 1968
Co-fractionation Homo sapiens
9 RPL30 6156
Co-fractionation Homo sapiens
10 GIT2 9815
Two-hybrid Homo sapiens
11 NOP56 10528
Co-fractionation Homo sapiens
12 DHX8 1659
Co-fractionation Homo sapiens
13 KIF20A 10112
Affinity Capture-MS Homo sapiens
14 MAD2L1BP  
Affinity Capture-MS Homo sapiens
15 CAND1 55832
Affinity Capture-MS Homo sapiens
16 FAXC  
Affinity Capture-MS Homo sapiens
17 WDR36 134430
Co-fractionation Homo sapiens
18 URI1  
Affinity Capture-MS Homo sapiens
19 POLR3K  
Affinity Capture-MS Homo sapiens
20 APEX1 328
Affinity Capture-RNA Homo sapiens
21 NFKB1 4790
Co-fractionation Homo sapiens
22 WDR76  
Co-purification Homo sapiens
Affinity Capture-MS Homo sapiens
23 USO1 8615
Co-fractionation Homo sapiens
24 NOLC1 9221
Affinity Capture-Western Homo sapiens
25 COPB2 9276
Co-fractionation Homo sapiens
26 POLR2I 5438
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
27 TWISTNB  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
28 POLR1E  
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 ABCE1 6059
Affinity Capture-MS Homo sapiens
30 CRCP  
Affinity Capture-MS Homo sapiens
31 TEX19  
Affinity Capture-MS Homo sapiens
32 ATG7 10533
Affinity Capture-MS Homo sapiens
33 DDX18 8886
Co-fractionation Homo sapiens
34 POLR2C 5432
Affinity Capture-MS Homo sapiens
35 GPN1  
Affinity Capture-MS Homo sapiens
36 POLR2M  
Affinity Capture-MS Homo sapiens
37 PFDN6 10471
Affinity Capture-MS Homo sapiens
38 ERBB2 2064
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
39 FBXW5  
Affinity Capture-MS Homo sapiens
40 KIF14 9928
Affinity Capture-MS Homo sapiens
41 GPN3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 POLR2K  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 TCOF1 6949
Affinity Capture-Western Homo sapiens
44 MED20 9477
Two-hybrid Homo sapiens
45 UXT 8409
Affinity Capture-MS Homo sapiens
46 RPL9 6133
Co-fractionation Homo sapiens
47 USP11 8237
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 ZNF207 7756
Two-hybrid Homo sapiens
49 PTPN21 11099
Affinity Capture-MS Homo sapiens
50 RPA2 6118
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
51 PFDN2 5202
Affinity Capture-MS Homo sapiens
52 NTRK1 4914
Affinity Capture-MS Homo sapiens
53 IFI16 3428
Affinity Capture-MS Homo sapiens
54 LRPPRC 10128
Co-fractionation Homo sapiens
55 ACTB 60
Co-localization Homo sapiens
56 HECTD1 25831
Affinity Capture-MS Homo sapiens
57 LZTR1 8216
Two-hybrid Homo sapiens
58 POLR2B 5431
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
59 RPRD2  
Affinity Capture-MS Homo sapiens
60 TCP10  
Affinity Capture-MS Homo sapiens
61 POLR3A 11128
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
62 POLR2H 5437
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
63 RC3H2  
Affinity Capture-MS Homo sapiens
64 TRIM10  
Affinity Capture-MS Homo sapiens
65 POLR2E 5434
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
66 USP36  
Affinity Capture-MS Homo sapiens
67 RPRD1A  
Affinity Capture-MS Homo sapiens
68 POLR2D  
Affinity Capture-MS Homo sapiens
69 POLR2A 5430
Affinity Capture-MS Homo sapiens
70 POLR3E 55718
Affinity Capture-MS Homo sapiens
71 MYO1C 4641
Affinity Capture-Western Homo sapiens
72 FKBP5 2289
Affinity Capture-MS Homo sapiens
73 RPA3 6119
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
74 SOHLH1  
Affinity Capture-MS Homo sapiens
75 AMZ1  
Affinity Capture-MS Homo sapiens
76 AKT2 208
Two-hybrid Homo sapiens
77 RPAP3 79657
Affinity Capture-MS Homo sapiens
78 POLR1A  
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
79 RPL11 6135
Co-fractionation Homo sapiens
80 CCND1 595
Two-hybrid Homo sapiens
81 TSR1 55720
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
82 C9orf72  
Affinity Capture-MS Homo sapiens
83 RPL37 6167
Two-hybrid Homo sapiens
84 ARRB2 409
Affinity Capture-MS Homo sapiens
85 POLR2F  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
86 SIRT6  
Affinity Capture-MS Homo sapiens
87 PARP1 142
Proximity Label-MS Homo sapiens
88 POLR2J  
Affinity Capture-MS Homo sapiens
89 POLR2L 5441
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
90 TRIM52  
Affinity Capture-MS Homo sapiens
91 POLR1C 9533
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
92 WDR92 116143
Affinity Capture-MS Homo sapiens
93 RPA1 6117
Affinity Capture-MS Homo sapiens
94 GFOD1  
Affinity Capture-MS Homo sapiens
95 RPAP2  
Affinity Capture-MS Homo sapiens
96 TBL3 10607
Co-fractionation Homo sapiens
97 TAF1D  
Affinity Capture-MS Homo sapiens
98 RPS5 6193
Co-fractionation Homo sapiens
99 SKAP1 8631
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
100 ACTA1 58
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
101 LDB1  
Two-hybrid Homo sapiens
102 NCL 4691
Affinity Capture-MS Homo sapiens
103 HDAC6 10013
Two-hybrid Homo sapiens
104 LOC554223  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
105 POLR3D  
Affinity Capture-MS Homo sapiens
106 POLR3B 55703
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
107 RPRD1B 58490
Affinity Capture-MS Homo sapiens
108 PIH1D1  
Affinity Capture-MS Homo sapiens
109 ZNRD1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
110 EEFSEC 60678
Co-fractionation Homo sapiens
111 KRAS 3845
Negative Genetic Homo sapiens
112 POLR1D 51082
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
113 POLR3H  
Affinity Capture-MS Homo sapiens
114 RC3H1 149041
Affinity Capture-MS Homo sapiens
115 IKBKB 3551
Co-fractionation Homo sapiens
116 NEMF 9147
Co-fractionation Homo sapiens
117 PDRG1  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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