Gene description for KIF3A
Gene name kinesin family member 3A
Gene symbol KIF3A
Other names/aliases FLA10
KLP-20
Species Homo sapiens
 Database cross references - KIF3A
ExoCarta ExoCarta_11127
Vesiclepedia VP_11127
Entrez Gene 11127
HGNC 6319
MIM 604683
UniProt Q9Y496  
 KIF3A identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Urine 19056867    
Urine 21595033    
Urine 21595033    
Urine 21595033    
 Gene ontology annotations for KIF3A
Molecular Function
    microtubule motor activity GO:0003777 IBA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    microtubule binding GO:0008017 IBA
    plus-end-directed microtubule motor activity GO:0008574 TAS
    ATP hydrolysis activity GO:0016887 IBA
    protein phosphatase binding GO:0019903 IPI
    spectrin binding GO:0030507 IDA
    small GTPase binding GO:0031267 IPI
Biological Process
    organelle organization GO:0006996 TAS
    anterograde axonal transport GO:0008089 IBA
    centriole-centriole cohesion GO:0010457 ISS
    protein transport GO:0015031 IMP
    microtubule anchoring at centrosome GO:0034454 ISS
    cilium assembly GO:0060271 IBA
    cilium assembly GO:0060271 ISS
    plus-end-directed vesicle transport along microtubule GO:0072383 TAS
    protein localization to cell junction GO:1902414 IMP
Subcellular Localization
    cytoplasm GO:0005737 IBA
    centrosome GO:0005813 IDA
    centriole GO:0005814 IDA
    cytosol GO:0005829 TAS
    kinesin complex GO:0005871 IBA
    microtubule GO:0005874 IBA
    spindle microtubule GO:0005876 IDA
    cilium GO:0005929 ISS
    cilium GO:0005929 TAS
    microtubule cytoskeleton GO:0015630 IDA
    kinesin II complex GO:0016939 IDA
    extracellular exosome GO:0070062 HDA
    ciliary tip GO:0097542 TAS
    axon cytoplasm GO:1904115 IEA
 Experiment description of studies that identified KIF3A in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 211
MISEV standards
EM
Biophysical techniques
TSG101|Alix|EpCAM|TFRC
Enriched markers
cytochrome c|GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 212
MISEV standards
CEM
Biophysical techniques
TSG101|Alix|EpCAM|TFRC
Enriched markers
Cytochrome C|GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 63
MISEV standards
Biophysical techniques
AQP2
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
8
Experiment ID 193
MISEV standards
EM
Biophysical techniques
CD63|CD9
Enriched markers
PHB
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 194
MISEV standards
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of basement membrane nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 195
MISEV standards
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of early IgA nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for KIF3A
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 LIG4 3981
Two-hybrid Homo sapiens
2 NUF2  
Affinity Capture-MS Homo sapiens
3 LYG2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 FAM26D  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 CAMSAP1 157922
Affinity Capture-MS Homo sapiens
6 FAM192A  
Affinity Capture-MS Homo sapiens
7 CEP170 9859
Affinity Capture-MS Homo sapiens
8 MID1IP1  
Affinity Capture-MS Homo sapiens
9 CTNNBL1 56259
Affinity Capture-MS Homo sapiens
10 TNFRSF8 943
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 DCTN2 10540
Proximity Label-MS Homo sapiens
12 VHL  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
13 APP 351
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
14 ATXN7L1 222255
Affinity Capture-MS Homo sapiens
15 CASP1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 C10orf82  
Affinity Capture-MS Homo sapiens
17 CAPN12  
Affinity Capture-MS Homo sapiens
18 DUSP14 11072
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 ACPP 55
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 KIFAP3 22920
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 EIF5B 9669
Co-fractionation Homo sapiens
22 BOD1L1 259282
Affinity Capture-MS Homo sapiens
23 PGRMC2 10424
Affinity Capture-MS Homo sapiens
24 RGS20 8601
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 Kif3c  
Affinity Capture-MS Mus musculus
26 ATG9B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 USP7 7874
Affinity Capture-MS Homo sapiens
28 EXOC3 11336
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 C15orf59  
Affinity Capture-MS Homo sapiens
30 XRCC4  
Affinity Capture-MS Homo sapiens
31 IKBIP 121457
Affinity Capture-MS Homo sapiens
32 PRKCI 5584
Co-fractionation Homo sapiens
33 PARK2  
Affinity Capture-MS Homo sapiens
34 BEX4  
Affinity Capture-MS Homo sapiens
35 SSX2  
Affinity Capture-MS Homo sapiens
36 DTNBP1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 MKRN2 23609
Affinity Capture-RNA Homo sapiens
38 RAN 5901
Affinity Capture-MS Homo sapiens
39 NTRK1 4914
Affinity Capture-MS Homo sapiens
40 LYPLA2 11313
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 PLEKHO2 80301
Affinity Capture-MS Homo sapiens
42 NFYC 4802
Affinity Capture-MS Homo sapiens
43 APTX  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 GAK 2580
Affinity Capture-MS Homo sapiens
45 KIF3B 9371
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 LRRC15 131578
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 DISC1 27185
Two-hybrid Homo sapiens
48 WHAMMP3  
Affinity Capture-MS Homo sapiens
49 RNF181  
Affinity Capture-MS Homo sapiens
50 HEPHL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 CTNNB1 1499
Affinity Capture-MS Homo sapiens
52 CARTPT  
Affinity Capture-MS Homo sapiens
53 SLC9A3R2 9351
Affinity Capture-MS Homo sapiens
54 TYW3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
55 GTPBP1 9567
Affinity Capture-MS Homo sapiens
56 OBFC1 79991
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 H3F3B 3021
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
58 ANXA8 653145
Affinity Capture-MS Homo sapiens
59 SERPINB5 5268
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
60 BAIAP2L1 55971
Affinity Capture-MS Homo sapiens
61 PLEKHA5 54477
Two-hybrid Homo sapiens
62 AURKB 9212
Affinity Capture-MS Homo sapiens
63 MFHAS1 9258
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
64 PADI3 51702
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
65 KIF1BP 26128
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 SSNA1 8636
Affinity Capture-MS Homo sapiens
67 SELENBP1 8991
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
68 DNAJC9 23234
Affinity Capture-MS Homo sapiens
69 FAM153A  
Affinity Capture-MS Homo sapiens
70 HSDL2 84263
Affinity Capture-MS Homo sapiens
71 DNPEP 23549
Affinity Capture-MS Homo sapiens
72 DHX34 9704
Affinity Capture-MS Homo sapiens
73 PLCD1 5333
Affinity Capture-MS Homo sapiens
74 PI4K2A 55361
Affinity Capture-MS Homo sapiens
75 KIF3C 3797
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
76 PUS1 80324
Affinity Capture-MS Homo sapiens
77 MAP3K10  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
78 PREP 5550
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
79 ACBD5 91452
Proximity Label-MS Homo sapiens
80 PKP3 11187
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
81 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
82 FASN 2194
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
83 ZNF785  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
84 GRB2 2885
Two-hybrid Homo sapiens
85 UBA6 55236
Affinity Capture-MS Homo sapiens
86 MAP1S 55201
Affinity Capture-MS Homo sapiens
87 ALOX15B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
88 HENMT1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
89 RPA1 6117
Affinity Capture-MS Homo sapiens
90 ARL8A 127829
Affinity Capture-MS Homo sapiens
91 TIMM10  
Affinity Capture-MS Homo sapiens
92 VSIG8  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
93 PLEKHJ1  
Affinity Capture-MS Homo sapiens
94 PAFAH2 5051
Affinity Capture-MS Homo sapiens
95 MAGEA9  
Affinity Capture-MS Homo sapiens
96 PKP1 5317
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
97 NEK1  
Two-hybrid Homo sapiens
98 IDH2 3418
Affinity Capture-MS Homo sapiens
99 ARRB1 408
Affinity Capture-MS Homo sapiens
100 DSG4 147409
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
101 MAP3K11 4296
Two-hybrid Homo sapiens
102 RANBP2 5903
Affinity Capture-Western Homo sapiens
103 TRIM29 23650
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
104 SSH2  
Affinity Capture-MS Homo sapiens
105 LURAP1  
Affinity Capture-MS Homo sapiens
106 NOTCH1 4851
Affinity Capture-MS Homo sapiens
107 C17orf59 54785
Proximity Label-MS Homo sapiens
108 MAP1LC3B2  
Affinity Capture-MS Homo sapiens
109 INTU  
Affinity Capture-MS Homo sapiens
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