Gene description for CLINT1
Gene name clathrin interactor 1
Gene symbol CLINT1
Other names/aliases CLINT
ENTH
EPN4
EPNR
Species Homo sapiens
 Database cross references - CLINT1
ExoCarta ExoCarta_9685
Entrez Gene 9685
HGNC 23186
MIM 607265
UniProt Q14677  
 CLINT1 identified in exosomes derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Squamous carcinoma cells 20124223    
Thymus 23844026    
 Gene ontology annotations for CLINT1
Molecular Function
    clathrin binding GO:0030276 IPI
    protein binding GO:0005515 IPI
    lipid binding GO:0008289 IEA
Biological Process
    endocytosis GO:0006897 IEA
    clathrin coat assembly GO:0048268 IEA
    post-Golgi vesicle-mediated transport GO:0006892 TAS
    membrane organization GO:0061024 TAS
Subcellular Localization
    Golgi apparatus GO:0005794 IDA
    perinuclear region of cytoplasm GO:0048471 IEA
    clathrin-coated vesicle GO:0030136 IEA
    nucleoplasm GO:0005654 IDA
    cytosol GO:0005829 TAS
    membrane GO:0016020 IDA
    intracellular membrane-bounded organelle GO:0043231 IDA
 Experiment description of studies that identified CLINT1 in exosomes
1
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 191
ISEV standards
✘ EV Biophysical techniques
✔
Alix
EV Cytosolic markers
✔
CD81|CD9
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK.
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 217
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
CD81|CD9|CD63
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for CLINT1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 AP1B1 162
Reconstituted Complex Homo sapiens
2 AP1G1 164
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
3 DNM1 1759
Reconstituted Complex Homo sapiens
4 CLTC 1213
Reconstituted Complex Homo sapiens
5 AP2B1 163
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
6 EPS15 2060
Reconstituted Complex Homo sapiens
7 GGA2  
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
8 AP2A1 160
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
View the network image/svg+xml
 Pathways in which CLINT1 is involved
PathwayEvidenceSource
Golgi Associated Vesicle Biogenesis TAS Reactome





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