Gene description for SMAD3
Gene name SMAD family member 3
Gene symbol SMAD3
Other names/aliases HSPC193
HsT17436
JV15-2
LDS1C
LDS3
MADH3
Species Homo sapiens
 Database cross references - SMAD3
ExoCarta ExoCarta_4088
Vesiclepedia VP_4088
Entrez Gene 4088
HGNC 6769
MIM 603109
UniProt P84022  
 SMAD3 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for SMAD3
Molecular Function
    transcription cis-regulatory region binding GO:0000976 IDA
    RNA polymerase II cis-regulatory region sequence-specific DNA binding GO:0000978 IBA
    RNA polymerase II cis-regulatory region sequence-specific DNA binding GO:0000978 IDA
    DNA-binding transcription factor activity, RNA polymerase II-specific GO:0000981 IBA
    DNA-binding transcription factor activity, RNA polymerase II-specific GO:0000981 IDA
    DNA-binding transcription factor activity, RNA polymerase II-specific GO:0000981 ISA
    cis-regulatory region sequence-specific DNA binding GO:0000987 IC
    cis-regulatory region sequence-specific DNA binding GO:0000987 IDA
    cis-regulatory region sequence-specific DNA binding GO:0000987 IMP
    DNA-binding transcription repressor activity GO:0001217 IDA
    transcription corepressor binding GO:0001222 IPI
    transcription coactivator binding GO:0001223 IPI
    DNA-binding transcription activator activity, RNA polymerase II-specific GO:0001228 IDA
    DNA-binding transcription activator activity, RNA polymerase II-specific GO:0001228 IMP
    DNA-binding transcription activator activity, RNA polymerase II-specific GO:0001228 ISS
    DNA binding GO:0003677 IDA
    DNA-binding transcription factor activity GO:0003700 IDA
    DNA-binding transcription factor activity GO:0003700 IDA
    DNA-binding transcription factor activity GO:0003700 IMP
    transforming growth factor beta receptor binding GO:0005160 IPI
    protein binding GO:0005515 IPI
    collagen binding GO:0005518 IEA
    beta-catenin binding GO:0008013 IEA
    zinc ion binding GO:0008270 IDA
    nuclear receptor binding GO:0016922 IPI
    DEAD/H-box RNA helicase binding GO:0017151 IPI
    protein kinase binding GO:0019901 IPI
    phosphatase binding GO:0019902 IPI
    chromatin DNA binding GO:0031490 IEA
    ubiquitin protein ligase binding GO:0031625 IPI
    nuclear mineralocorticoid receptor binding GO:0031962 IPI
    sterol response element binding GO:0032810 IGI
    nuclear glucocorticoid receptor binding GO:0035259 IPI
    identical protein binding GO:0042802 IPI
    protein homodimerization activity GO:0042803 IPI
    ubiquitin binding GO:0043130 IDA
    bHLH transcription factor binding GO:0043425 IPI
    sequence-specific DNA binding GO:0043565 IDA
    RNA polymerase II-specific DNA-binding transcription factor binding GO:0061629 IPI
    co-SMAD binding GO:0070410 IPI
    I-SMAD binding GO:0070411 IBA
    R-SMAD binding GO:0070412 IPI
    DNA-binding transcription factor binding GO:0140297 IPI
    promoter-specific chromatin binding GO:1990841 IEA
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 IDA
    negative regulation of transcription by RNA polymerase II GO:0000122 IMP
    ureteric bud development GO:0001657 IEA
    response to hypoxia GO:0001666 IMP
    in utero embryonic development GO:0001701 IEA
    mesoderm formation GO:0001707 IEA
    somitogenesis GO:0001756 IEA
    liver development GO:0001889 IEA
    heart looping GO:0001947 IEA
    osteoblast development GO:0002076 IEA
    immune system development GO:0002520 IEA
    regulation of DNA-templated transcription GO:0006355 IDA
    regulation of DNA-templated transcription GO:0006355 NAS
    regulation of transcription by RNA polymerase II GO:0006357 IDA
    immune response GO:0006955 IMP
    transforming growth factor beta receptor signaling pathway GO:0007179 IBA
    transforming growth factor beta receptor signaling pathway GO:0007179 IDA
    transforming growth factor beta receptor signaling pathway GO:0007179 IMP
    transforming growth factor beta receptor signaling pathway GO:0007179 NAS
    JNK cascade GO:0007254 IEA
    endoderm development GO:0007492 IEA
    cell population proliferation GO:0008283 IEA
    negative regulation of cell population proliferation GO:0008285 IMP
    anatomical structure morphogenesis GO:0009653 IBA
    embryonic pattern specification GO:0009880 IEA
    positive regulation of gene expression GO:0010628 IDA
    negative regulation of gene expression GO:0010629 IMP
    positive regulation of epithelial to mesenchymal transition GO:0010718 IDA
    positive regulation of epithelial to mesenchymal transition GO:0010718 IMP
    regulation of striated muscle tissue development GO:0016202 IEA
    regulation of transforming growth factor beta receptor signaling pathway GO:0017015 IEA
    signal transduction involved in regulation of gene expression GO:0023019 IEA
    cell differentiation GO:0030154 IBA
    negative regulation of ossification GO:0030279 IDA
    negative regulation of cell growth GO:0030308 IDA
    adrenal gland development GO:0030325 IEA
    positive regulation of cell migration GO:0030335 IEA
    positive regulation of bone mineralization GO:0030501 IEA
    thyroid gland development GO:0030878 IEA
    primary miRNA processing GO:0031053 TAS
    positive regulation of chondrocyte differentiation GO:0032332 IEA
    positive regulation of interleukin-1 beta production GO:0032731 IEA
    regulation of transforming growth factor beta2 production GO:0032909 IMP
    positive regulation of transforming growth factor beta3 production GO:0032916 IEA
    activin receptor signaling pathway GO:0032924 IBA
    activin receptor signaling pathway GO:0032924 IMP
    activin receptor signaling pathway GO:0032924 NAS
    negative regulation of osteoblast proliferation GO:0033689 IEA
    nodal signaling pathway GO:0038092 IMP
    wound healing GO:0042060 TAS
    T cell activation GO:0042110 IEA
    negative regulation of protein catabolic process GO:0042177 IEA
    positive regulation of protein import into nucleus GO:0042307 NAS
    negative regulation of apoptotic process GO:0043066 IEA
    cell-cell junction organization GO:0045216 IMP
    positive regulation of nitric oxide biosynthetic process GO:0045429 IDA
    negative regulation of fat cell differentiation GO:0045599 IDA
    negative regulation of osteoblast differentiation GO:0045668 IEA
    positive regulation of DNA-templated transcription GO:0045893 IDA
    positive regulation of transcription by RNA polymerase II GO:0045944 IBA
    positive regulation of transcription by RNA polymerase II GO:0045944 IDA
    positive regulation of transcription by RNA polymerase II GO:0045944 IMP
    paraxial mesoderm morphogenesis GO:0048340 IEA
    developmental growth GO:0048589 IEA
    embryonic foregut morphogenesis GO:0048617 IEA
    embryonic cranial skeleton morphogenesis GO:0048701 IEA
    regulation of epithelial cell proliferation GO:0050678 IEA
    negative regulation of inflammatory response GO:0050728 IEA
    regulation of immune response GO:0050776 IEA
    protein stabilization GO:0050821 IEA
    positive regulation of positive chemotaxis GO:0050927 IEA
    positive regulation of DNA-binding transcription factor activity GO:0051091 NAS
    negative regulation of cytosolic calcium ion concentration GO:0051481 IDA
    positive regulation of stress fiber assembly GO:0051496 IEA
    positive regulation of focal adhesion assembly GO:0051894 IEA
    pericardium development GO:0060039 IEA
    transdifferentiation GO:0060290 IEA
    positive regulation of SMAD protein signal transduction GO:0060391 IMP
    SMAD protein signal transduction GO:0060395 IBA
    SMAD protein signal transduction GO:0060395 IDA
    SMAD protein signal transduction GO:0060395 IMP
    SMAD protein signal transduction GO:0060395 NAS
    negative regulation of wound healing GO:0061045 IEA
    negative regulation of lung blood pressure GO:0061767 IEA
    lens fiber cell differentiation GO:0070306 IEA
    cellular response to glucose stimulus GO:0071333 IEA
    cellular response to transforming growth factor beta stimulus GO:0071560 IDA
    positive regulation of canonical Wnt signaling pathway GO:0090263 IEA
    apoptotic signaling pathway GO:0097190 IMP
    extrinsic apoptotic signaling pathway GO:0097191 IMP
    cellular response to virus GO:0098586 IEA
    positive regulation of extracellular matrix assembly GO:1901203 IDA
    positive regulation of extracellular matrix assembly GO:1901203 IMP
    regulation of miRNA transcription GO:1902893 IC
    negative regulation of miRNA transcription GO:1902894 IMP
    positive regulation of miRNA transcription GO:1902895 IDA
    positive regulation of miRNA transcription GO:1902895 IMP
    positive regulation of miRNA transcription GO:1902895 IMP
    negative regulation of cardiac muscle hypertrophy in response to stress GO:1903243 IEA
    response to angiotensin GO:1990776 IMP
Subcellular Localization
    chromatin GO:0000785 IDA
    chromatin GO:0000785 ISA
    nucleus GO:0005634 IDA
    nucleus GO:0005634 IGI
    nuclear inner membrane GO:0005637 IDA
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    transcription regulator complex GO:0005667 IDA
    transcription regulator complex GO:0005667 IPI
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 IGI
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IEA
    receptor complex GO:0043235 IMP
    SMAD protein complex GO:0071141 IDA
    heteromeric SMAD protein complex GO:0071144 IBA
    heteromeric SMAD protein complex GO:0071144 IDA
    heteromeric SMAD protein complex GO:0071144 NAS
 Experiment description of studies that identified SMAD3 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for SMAD3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ITGB1 3688
Co-fractionation Homo sapiens
2 HDAC4  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
3 AATF  
Two-hybrid Homo sapiens
4 SKI 6497
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
5 AES 166
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
6 BRCA1 672
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
7 ZC3H12A  
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
8 TGM2 7052
Two-hybrid Homo sapiens
9 SNRNP70 6625
Two-hybrid Homo sapiens
10 MTF2  
Two-hybrid Homo sapiens
11 NUP153 9972
Reconstituted Complex Homo sapiens
12 C10orf54 64115
Two-hybrid Homo sapiens
13 CPSF7 79869
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
14 RGCC 28984
Affinity Capture-Western Homo sapiens
15 ZMYND11 10771
Two-hybrid Homo sapiens
16 NCOA3  
Affinity Capture-MS Homo sapiens
17 USP33  
Affinity Capture-MS Homo sapiens
18 UCHL5 51377
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
19 SMAD5 4090
Affinity Capture-MS Homo sapiens
20 PRTN3 5657
Affinity Capture-Western Homo sapiens
21 BTRC 8945
Affinity Capture-Western Homo sapiens
22 ZFYVE9  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 MTA2 9219
Co-fractionation Homo sapiens
24 CBL 867
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
25 NEDD9 4739
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
26 TCP1 6950
Affinity Capture-MS Homo sapiens
27 ZNF83  
Two-hybrid Homo sapiens
28 PABPC1 26986
Affinity Capture-MS Homo sapiens
29 CDH1 999
Co-fractionation Homo sapiens
30 FOXL2  
Affinity Capture-Western Homo sapiens
31 YAP1 10413
Affinity Capture-Western Homo sapiens
32 C1orf116 79098
Affinity Capture-MS Homo sapiens
33 GMEB1  
Two-hybrid Homo sapiens
34 BMP7 655
Co-localization Homo sapiens
35 MEOX2  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
36 MAPK8 5599
Co-localization Homo sapiens
37 TMED1 11018
Two-hybrid Homo sapiens
38 MED15  
Affinity Capture-Western Homo sapiens
39 SQRDL 58472
Affinity Capture-MS Homo sapiens
40 FOXH1  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Protein-peptide Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
41 EIF4B 1975
Reconstituted Complex Homo sapiens
42 PAX6  
Affinity Capture-Western Homo sapiens
43 RELA 5970
Two-hybrid Homo sapiens
44 PLAGL1  
Two-hybrid Homo sapiens
45 EPAS1  
Co-localization Homo sapiens
Co-localization Homo sapiens
Two-hybrid Homo sapiens
46 ERBB2IP 55914
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
47 PIAS2  
Reconstituted Complex Homo sapiens
48 TEKT4  
Two-hybrid Homo sapiens
49 FOXO1  
Affinity Capture-Western Homo sapiens
50 Dok1  
Affinity Capture-Western Mus musculus
51 RUNX3  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
52 STRAP 11171
Affinity Capture-Western Homo sapiens
Phenotypic Suppression Homo sapiens
Affinity Capture-Western Homo sapiens
Phenotypic Suppression Homo sapiens
53 CYP11A1  
Two-hybrid Homo sapiens
54 ETS1  
Co-localization Homo sapiens
55 HSPA4 3308
Affinity Capture-MS Homo sapiens
56 AKT1 207
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
57 GRIP1  
Affinity Capture-Western Homo sapiens
58 CAMK2G 818
Co-localization Homo sapiens
59 STUB1 10273
Reconstituted Complex Homo sapiens
60 DNMT3L  
Reconstituted Complex Homo sapiens
61 DVL1 1855
Reconstituted Complex Homo sapiens
62 TANK 10010
Two-hybrid Homo sapiens
63 DAB2 1601
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
64 SKIL  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
65 CDK8 1024
Biochemical Activity Homo sapiens
66 KMT2B  
Two-hybrid Homo sapiens
67 Fbxo3  
Affinity Capture-Luminescence Mus musculus
68 ZEB1  
Affinity Capture-Western Homo sapiens
69 Rras2 66922
Affinity Capture-Luminescence Mus musculus
70 WWP2 11060
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
71 DDX3X 1654
Affinity Capture-MS Homo sapiens
72 Sox9  
Reconstituted Complex Rattus norvegicus
Reconstituted Complex Rattus norvegicus
73 APOB 338
Two-hybrid Homo sapiens
74 CEBPB  
Reconstituted Complex Homo sapiens
75 SNW1 22938
Phenotypic Enhancement Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
76 NFIC 4782
Affinity Capture-Western Homo sapiens
77 SMURF2 64750
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Luminescence Homo sapiens
78 BLZF1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
79 NCOA6  
Two-hybrid Homo sapiens
80 ST6GALNAC2  
Two-hybrid Homo sapiens
81 UCHL1 7345
Affinity Capture-Western Homo sapiens
82 JUND 3727
Co-fractionation Homo sapiens
Reconstituted Complex Homo sapiens
83 TRIM33 51592
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
84 RNMT 8731
Co-fractionation Homo sapiens
85 PLG 5340
Two-hybrid Homo sapiens
86 Wee1  
Affinity Capture-Luminescence Mus musculus
87 YTHDF2 51441
Affinity Capture-MS Homo sapiens
88 TGFBR1 7046
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
89 S100A4 6275
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
90 ZMIZ1 57178
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
91 TCF12  
Affinity Capture-Western Homo sapiens
92 HEY1  
Two-hybrid Homo sapiens
93 Rgs3  
Affinity Capture-Luminescence Mus musculus
94 UGT1A10  
Affinity Capture-MS Homo sapiens
95 GGA1 26088
Two-hybrid Homo sapiens
96 TMEM192 201931
Affinity Capture-MS Homo sapiens
97 LDLRAD4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
98 CUL1 8454
Affinity Capture-Western Homo sapiens
99 USP7 7874
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
100 GSK3B 2932
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
101 Wwp2  
Affinity Capture-Luminescence Mus musculus
102 HOXC11  
Two-hybrid Homo sapiens
103 KANSL1  
Two-hybrid Homo sapiens
104 RUNX1  
Affinity Capture-Western Homo sapiens
105 RBX1 9978
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
106 FBLN1 2192
Two-hybrid Homo sapiens
107 CDKN1A  
Affinity Capture-Western Homo sapiens
108 RPL4 6124
Affinity Capture-MS Homo sapiens
109 ZBTB16  
Two-hybrid Homo sapiens
110 BACH1 571
Affinity Capture-Western Homo sapiens
111 TRIM24  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
112 CFDP1  
Two-hybrid Homo sapiens
113 ZEB2  
Affinity Capture-Western Homo sapiens