Gene description for ISG15
Gene name ISG15 ubiquitin-like modifier
Gene symbol ISG15
Other names/aliases G1P2
Species Homo sapiens
 Database cross references - ISG15
ExoCarta ExoCarta_9636
Entrez Gene 9636
HGNC 4053
MIM 147571
UniProt P05161  
 ISG15 identified in exosomes derived from the following tissue/cell type
Endothelial cells 26027894    
Squamous carcinoma cells 20124223    
 Gene ontology annotations for ISG15
Molecular Function
    protein tag GO:0031386 IEA
    protein binding GO:0005515 IPI
Biological Process
    response to type I interferon GO:0034340 IDA
    negative regulation of viral genome replication GO:0045071 IMP
    negative regulation of protein ubiquitination GO:0031397 IDA
    ISG15-protein conjugation GO:0032020 IMP
    modification-dependent protein catabolic process GO:0019941 IEA
    innate immune response GO:0045087 TAS
    positive regulation of erythrocyte differentiation GO:0045648 IEA
    type I interferon signaling pathway GO:0060337 TAS
    DNA repair GO:0006281 TAS
    cytokine-mediated signaling pathway GO:0019221 TAS
    negative regulation of type I interferon production GO:0032480 TAS
    viral process GO:0016032 IEA
    translesion synthesis GO:0019985 TAS
    regulation of interferon-gamma production GO:0032649 IMP
    defense response to bacterium GO:0042742 IMP
    defense response to virus GO:0051607 IMP
Subcellular Localization
    cytosol GO:0005829 TAS
    extracellular region GO:0005576 IDA
    nucleoplasm GO:0005654 TAS
 Experiment description of studies that identified ISG15 in exosomes
Experiment ID 226
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting
Authors Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
Experiment ID 191
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK.
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for ISG15
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 USP18  
Two-hybrid Homo sapiens
2 UBA7 7318
Two-hybrid Homo sapiens
3 ELF3 1999
Affinity Capture-MS Homo sapiens
View the network image/svg+xml

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