ExoCarta: Gene summary

Credits | Contact

Gene description for SNRPB2
Gene name	small nuclear ribonucleoprotein polypeptide B
Gene symbol	SNRPB2
Other names/aliases	Msl1
Species	Homo sapiens
Database cross references - SNRPB2
ExoCarta	ExoCarta_6629
Vesiclepedia	VP_6629
Entrez Gene	6629
HGNC	11155
MIM	603520
UniProt	P08579
SNRPB2 identified in exosomes derived from the following tissue/cell type
Chondrocytes	35931686
Chondrocytes	35931686
Ovarian cancer cells	23333927
Ovarian cancer cells	23333927
Thymus	23844026

Gene ontology annotations for SNRPB2

Molecular Function
	protein binding	GO:0005515	IPI
	U1 snRNA binding	GO:0030619	IBA
	snRNP binding	GO:0070990	IEA
Biological Process
	mRNA splicing, via spliceosome	GO:0000398	IBA
	mRNA splicing, via spliceosome	GO:0000398	IC
	mRNA splicing, via spliceosome	GO:0000398	IDA
	mRNA splicing, via spliceosome	GO:0000398	NAS
	U2-type prespliceosome assembly	GO:1903241	NAS
Subcellular Localization
	fibrillar center	GO:0001650	IDA
	nucleus	GO:0005634	IDA
	nucleus	GO:0005634	NAS
	nucleoplasm	GO:0005654	TAS
	spliceosomal complex	GO:0005681	IDA
	spliceosomal complex	GO:0005681	NAS
	U2-type spliceosomal complex	GO:0005684	IDA
	U1 snRNP	GO:0005685	IBA
	U2 snRNP	GO:0005686	IDA
	U2 snRNP	GO:0005686	NAS
	nuclear speck	GO:0016607	IDA
	cytoplasmic ribonucleoprotein granule	GO:0036464	IDA
	U2-type precatalytic spliceosome	GO:0071005	IDA
	U2-type catalytic step 2 spliceosome	GO:0071007	IDA
	catalytic step 2 spliceosome	GO:0071013	IDA

Experiment description of studies that identified SNRPB2 in exosomes

Experiment ID

497

MISEV standards
✘	Biophysical techniques
✔ CD9\|CD151\|CD63\|CD81\|CD82\|FLOT1\|FLOT2\|GAPDH\|LAMP1\|LAMP2\|RAB5A\|SDCBP\|TFRC\|TSG101\|UCHL1	Enriched markers
✘	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

35931686

Organism

Homo sapiens

Experiment description

Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression

Authors

"Varela-EirÃn M, Carpintero-FernÃ¡ndez P, GuitiÃ¡n-CaamaÃ±o A, Varela-VÃ¡zquez A, GarcÃa-Yuste A, SÃ¡nchez-Temprano A, Bravo-LÃ³pez SB, YaÃ±ez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, MayÃ¡n MD."

Journal name

Cell Death Dis

Publication year

2022

Sample

Chondrocytes

Sample name

T/C-28a2

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

498

MISEV standards
✔ EM	Biophysical techniques
✔ CD9\|CD151\|CD63\|CD81\|CD82\|FLOT1\|FLOT2\|GAPDH\|LAMP1\|LAMP2\|RAB5A\|SDCBP\|TFRC\|TSG101\|UCHL1	Enriched markers
✘	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

35931686

Organism

Homo sapiens

Experiment description

Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression

Authors

Journal name

Cell Death Dis

Publication year

2022

Sample

Chondrocytes

Sample name

T/C-28a2

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

211

MISEV standards
✔ EM	Biophysical techniques
✔ TSG101\|Alix\|EpCAM\|TFRC	Enriched markers
✔ cytochrome c\|GOLGA2	Negative markers
✘	Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry

PubMed ID

23333927

Organism

Homo sapiens

Experiment description

Characterization and proteomic analysis of ovarian cancer-derived exosomes.

Authors

"Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."

Journal name

J Proteomics

Publication year

2013

Sample

Ovarian cancer cells

Sample name

IGROV1

Isolation/purification methods

Differential centrifugation
Ultracentrifugation
Sucrose density gradient

Flotation density

1.09-1.15 g/mL

Molecules identified in the study

Protein

Methods used in the study

Mass spectrometry

Experiment ID

212

MISEV standards
✔ CEM	Biophysical techniques
✔ TSG101\|Alix\|EpCAM\|TFRC	Enriched markers
✔ Cytochrome C\|GOLGA2	Negative markers
✘	Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry

PubMed ID

23333927

Organism

Homo sapiens

Experiment description

Characterization and proteomic analysis of ovarian cancer-derived exosomes.

Authors

"Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."

Journal name

J Proteomics

Publication year

2013

Sample

Ovarian cancer cells

Sample name

OVCAR-3

Isolation/purification methods

Differential centrifugation
Ultracentrifugation
Sucrose density gradient

Flotation density

1.09-1.15 g/mL

Molecules identified in the study

Protein

Methods used in the study

Mass spectrometry

Experiment ID

217

MISEV standards
✔ EM	Biophysical techniques
✔ TSG101\|CD81\|CD9\|CD63	Enriched markers
✘	Negative markers
✔ NTA	Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry

PubMed ID

23844026

Organism

Homo sapiens

Experiment description

Characterization of human thymic exosomes.

Authors

"Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."

Journal name

PLoS One

Publication year

2013

Sample

Thymus

Sample name

Normal-Thymus

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Mass spectrometry

Protein-protein interactions for SNRPB2

Protein Interactor

ExoCarta ID

Identification method

PubMed

Species

UBE2H

7328

Affinity Capture-MS

Homo sapiens

CCAR1

55749

Affinity Capture-MS

Homo sapiens

HNRNPC

3183

Co-fractionation

Homo sapiens

SNRPG

6637

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

SNRPE

6635

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Co-fractionation		Homo sapiens

COIL

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

DNAJC8

22826

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

ZNF346

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

RBM22

55696

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

PRPF8

10594

Affinity Capture-MS

Homo sapiens

PPIE

10450

Affinity Capture-MS

Homo sapiens

RPA2

6118

Proximity Label-MS

Homo sapiens

TGS1

Affinity Capture-MS

Homo sapiens

EBNA-LP

Affinity Capture-MS

GABPB2

Two-hybrid

Homo sapiens

DHX8

1659

Affinity Capture-MS

Homo sapiens

PDCD7

Affinity Capture-MS

Homo sapiens

RPL10

6134

Affinity Capture-MS

Homo sapiens

KIF20A

10112

Affinity Capture-MS

Homo sapiens

LSM4

25804

Affinity Capture-MS

Homo sapiens

PRPSAP2

5636

Co-fractionation

Homo sapiens

SSB

6741

Co-fractionation

Homo sapiens

PRPF3

Affinity Capture-MS

Homo sapiens

CALM1

801

Reconstituted Complex

Homo sapiens

PLEKHA2

59339

Affinity Capture-MS

Homo sapiens

PSMD10

5716

Co-fractionation

Homo sapiens

PRPF40A

55660

Affinity Capture-MS

Homo sapiens

SNRPF

6636

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

SNRPD2

6633

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Co-fractionation		Homo sapiens

SF3B5

83443

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Co-fractionation		Homo sapiens

CWF19L1

55280

Affinity Capture-MS

Homo sapiens

CWF19L2

Affinity Capture-MS

Homo sapiens

ERH

2079

Affinity Capture-MS

Homo sapiens

WTAP

9589

Affinity Capture-MS

Homo sapiens

DDX23

9416

Affinity Capture-MS

Homo sapiens

SNRNP70

6625

Co-fractionation		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

USP39

10713

Affinity Capture-MS

Homo sapiens

CRNKL1

51340

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

ICE2

Affinity Capture-MS

Homo sapiens

SF3B1

23451

Affinity Capture-MS		Homo sapiens
Co-fractionation		Homo sapiens
Co-fractionation		Homo sapiens
Co-fractionation		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

PUF60

22827

Co-fractionation

Homo sapiens

FBXW7

Synthetic Lethality

Homo sapiens

PRC1

9055

Affinity Capture-MS

Homo sapiens

MRPL12

6182

Affinity Capture-MS

Homo sapiens

SRRM2

23524

Co-fractionation

Homo sapiens

SNRPC

6631

Affinity Capture-MS

Homo sapiens

POU5F1

Affinity Capture-MS

Homo sapiens

IFIT3

3437

Affinity Capture-MS

Homo sapiens

OBSL1

23363

Affinity Capture-MS

Homo sapiens

MCM6

4175

Two-hybrid

Homo sapiens

SF3A2

8175

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Co-fractionation		Homo sapiens

FANCD2

Affinity Capture-MS

Homo sapiens

SNRPD3

6634

Affinity Capture-MS		Homo sapiens
Co-fractionation		Homo sapiens
Cross-Linking-MS (XL-MS)		Homo sapiens

HMG20B

Co-fractionation

Homo sapiens

ZBTB14

Two-hybrid		Homo sapiens
Two-hybrid		Homo sapiens

MECP2

4204

Affinity Capture-MS

Homo sapiens

CUL1

8454

Affinity Capture-MS

Homo sapiens

SLC4A1AP

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

MYCN

Affinity Capture-MS

Homo sapiens

PLS3

5358

Affinity Capture-MS

Homo sapiens

SLU7

Co-fractionation

Homo sapiens

EIF5B

9669

Affinity Capture-MS

Homo sapiens

CPNE3

8895

Affinity Capture-MS

Homo sapiens

RBM8A

9939

Affinity Capture-MS

Homo sapiens

KIF14

9928

Affinity Capture-MS

Homo sapiens

RNPC3

Affinity Capture-MS

Homo sapiens

FOXP1

27086

Protein-RNA

Homo sapiens

LIN28A

Affinity Capture-MS

Homo sapiens

E2F4

Affinity Capture-MS

Homo sapiens

GEMIN5

25929

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

MTUS2

23281

Two-hybrid

Homo sapiens

PCNP

57092

Affinity Capture-MS

Homo sapiens

POLD1

5424

Affinity Capture-MS

Homo sapiens

HIST1H4A

8359

Affinity Capture-MS

Homo sapiens

LIN28B

Affinity Capture-MS

Homo sapiens

ZNF207

7756

Affinity Capture-MS

Homo sapiens

MEPCE

56257

Affinity Capture-MS

Homo sapiens

PHAX

Affinity Capture-MS

Homo sapiens

SMEK1

55671

Affinity Capture-MS

Homo sapiens

ICE1

Affinity Capture-MS

Homo sapiens

ACIN1

22985

Affinity Capture-MS

Homo sapiens

GOLGA2

2801

Two-hybrid

Homo sapiens

SRRT

51593

Affinity Capture-MS

Homo sapiens

SNRNP200

23020

Co-fractionation		Homo sapiens
Affinity Capture-MS		Homo sapiens

WDR61

80349

Affinity Capture-MS

Homo sapiens

SNRPB

6628

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Cross-Linking-MS (XL-MS)		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Co-fractionation		Homo sapiens

DDX20

11218

Affinity Capture-MS

Homo sapiens

SNRPD1

6632

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Co-fractionation		Homo sapiens

PLRG1

5356

Affinity Capture-MS

Homo sapiens

U2AF2

11338

Co-fractionation

Homo sapiens

NSRP1

Affinity Capture-MS

Homo sapiens

SNRPN

6638

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

SF3B6

51639

Co-fractionation		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Co-fractionation		Homo sapiens

RPA4

Proximity Label-MS

Homo sapiens

ALYREF

10189

Affinity Capture-MS

Homo sapiens

DHX9

1660

Co-fractionation

Homo sapiens

DDX39B

7919

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Co-fractionation		Homo sapiens

SND1

27044

Affinity Capture-MS

Homo sapiens

PRPF31

26121

Co-fractionation

Homo sapiens

100

CEBPA

Protein-peptide

Homo sapiens

101

MYC

Affinity Capture-MS

Homo sapiens

102

SF3A3

10946

Affinity Capture-MS		Homo sapiens
Co-fractionation		Homo sapiens
Co-fractionation		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Co-fractionation		Homo sapiens

103

CRNN

49860

Affinity Capture-MS

Homo sapiens

104

GPATCH11

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

105

CCDC12

Affinity Capture-MS

Homo sapiens

106

UBE2N

7334

Affinity Capture-MS

Homo sapiens

107

SF3B2

10992

Affinity Capture-MS		Homo sapiens
Co-fractionation		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Co-fractionation		Homo sapiens

108

RBM39

9584

Affinity Capture-MS

Homo sapiens

109

SUGP1

Two-hybrid		Homo sapiens
Two-hybrid		Homo sapiens
Affinity Capture-Luminescence		Homo sapiens
Affinity Capture-MS		Homo sapiens

110

PPP4R2

Affinity Capture-MS		Homo sapiens
Proximity Label-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

111

SKP2

Co-fractionation

Homo sapiens

112

METTL14

Affinity Capture-MS

Homo sapiens

113

CHERP

10523

Affinity Capture-MS

Homo sapiens

114

XPO1

7514

Affinity Capture-MS

Homo sapiens

115

PNN

5411

Affinity Capture-MS

Homo sapiens

116

CD2BP2

10421

Affinity Capture-MS

Homo sapiens

117

HTATSF1

27336

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

118

SRSF1

6426

Co-fractionation

Homo sapiens

119

CCDC8

Affinity Capture-MS

Homo sapiens

120

CUL3

8452

Affinity Capture-MS

Homo sapiens

121

XAB2

56949

Affinity Capture-MS

Homo sapiens

122

PDIA3

2923

Affinity Capture-MS

Homo sapiens

123

HNRNPA1

3178

Co-fractionation

Homo sapiens

124

FUS

2521

Affinity Capture-MS

Homo sapiens

125

HMBOX1

Two-hybrid

Homo sapiens

126

HNRNPUL1

11100

Two-hybrid		Homo sapiens
Two-hybrid		Homo sapiens

127

SF1

7536

Co-fractionation

Homo sapiens

128

ZC3H11A

Affinity Capture-MS

Homo sapiens

129

ELP2

55250

Co-fractionation

Homo sapiens

130

SART1

9092

Affinity Capture-MS

Homo sapiens

131

SF3A1

10291

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Co-fractionation		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Co-fractionation		Homo sapiens

132

EFTUD2

9343

Affinity Capture-MS

Homo sapiens

133

RPA3

6119

Proximity Label-MS

Homo sapiens

134

MFAP1

Affinity Capture-MS

Homo sapiens

135

AQR

9716

Affinity Capture-MS

Homo sapiens

136

IFIT1

3434

Affinity Capture-MS

Homo sapiens

137

HECW2

Affinity Capture-MS

Homo sapiens

138

ZC3H18

Affinity Capture-MS

Homo sapiens

139

TRIM27

Two-hybrid		Homo sapiens
Two-hybrid		Homo sapiens

140

PHF5A

84844

Co-fractionation		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Co-fractionation		Homo sapiens

141

GABPB1

Two-hybrid

Homo sapiens

142

BCAS2

10286

Affinity Capture-MS

Homo sapiens

143

SART3

9733

Affinity Capture-MS

Homo sapiens

144

DDRGK1

65992

Affinity Capture-MS

Homo sapiens

145

RNF2

Affinity Capture-MS

Homo sapiens

146

SF3B3

23450

Affinity Capture-MS		Homo sapiens
Co-fractionation		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Co-fractionation		Homo sapiens

147

HSPD1

3329

Co-fractionation

Homo sapiens

148

RBM17

84991

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Co-fractionation		Homo sapiens

149

SOX2

Affinity Capture-MS

Homo sapiens

150

H2AFX

3014

Affinity Capture-MS

Homo sapiens

151

U2SURP

23350

Co-fractionation		Homo sapiens
Affinity Capture-MS		Homo sapiens

152

PA2G4

5036

Affinity Capture-MS

Homo sapiens

153

DDX46

9879

Co-fractionation

Homo sapiens

154

DHX15

1665

Co-fractionation

Homo sapiens

155

1431

Affinity Capture-MS

Homo sapiens

156

PLEKHA4

57664

Affinity Capture-MS

Homo sapiens

157

TOE1

114034

Affinity Capture-MS

Homo sapiens

158

SMN1

6606

Affinity Capture-MS

Homo sapiens

159

BKRF1

Affinity Capture-MS

160

TTC33

Affinity Capture-MS

Homo sapiens

161

SRRM1

10250

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

162

HOMEZ

Two-hybrid		Homo sapiens
Two-hybrid		Homo sapiens

163

PYGL

5836

Affinity Capture-MS

Homo sapiens

164

GPI

2821

Affinity Capture-MS

Homo sapiens

165

CDC40

Affinity Capture-MS

Homo sapiens

166

WBP11

Co-fractionation

Homo sapiens

167

SF3B4

10262

Co-fractionation		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

168

SAP18

10284

Affinity Capture-MS

Homo sapiens

169

RBM25

58517

Affinity Capture-MS

Homo sapiens

170

CUL5

8065

Affinity Capture-MS

Homo sapiens

171

SNRPA

6626

Affinity Capture-MS		Homo sapiens
Negative Genetic		Homo sapiens
Co-fractionation		Homo sapiens

172

SMNDC1

Co-fractionation

Homo sapiens

173

SNUPN

10073

Affinity Capture-MS

Homo sapiens

174

THRAP3

9967

Affinity Capture-MS

Homo sapiens

175

PRKRIP1

79706

Affinity Capture-MS

Homo sapiens

176

KLHL8

Affinity Capture-MS

Homo sapiens

177

Sf3a1

67465

Affinity Capture-MS

Mus musculus

178

PNMA1

9240

Two-hybrid

Homo sapiens

179

EIF4A3

9775

Co-fractionation		Homo sapiens
Affinity Capture-MS		Homo sapiens

180

CCDC97

Affinity Capture-MS

Homo sapiens

181

GEMIN4

50628

Affinity Capture-MS

Homo sapiens

182

CWC15

Co-fractionation

Homo sapiens

183

HNRNPM

4670

Co-fractionation

Homo sapiens

184

TRAP1

10131

Affinity Capture-MS

Homo sapiens

185

MAST2

Affinity Capture-MS

Homo sapiens

186

SNRPGP15

Co-fractionation

Homo sapiens

187

SNRNP40

9410

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

188

IDH2

3418

Affinity Capture-MS

Homo sapiens

189

ITSN2

50618

Affinity Capture-MS

Homo sapiens

190

EZH2

Affinity Capture-MS

Homo sapiens

191

TRIM23

373

Two-hybrid

Homo sapiens

192

CYLD

Affinity Capture-MS

Homo sapiens

193

ANXA5

308

Affinity Capture-MS

Homo sapiens

194

CIT

11113

Affinity Capture-MS

Homo sapiens

195

PRPF6

24148

Affinity Capture-MS

Homo sapiens

196

DDX42