Gene ontology annotations for PMPCA |
|
Experiment description of studies that identified PMPCA in exosomes |
1 |
Experiment ID |
489 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
|
Enriched markers |
✔
Canx
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
36408942
|
Organism |
Rattus norvegicus |
Experiment description |
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells |
Authors |
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ." |
Journal name |
Proteomics
|
Publication year |
2023 |
Sample |
Bone marrow mesenchymal stem cells |
Sample name |
BMSC - Passage 6 |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting Mass spectometry |
|
|
2 |
Experiment ID |
490 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
|
Enriched markers |
✔
Canx
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
36408942
|
Organism |
Rattus norvegicus |
Experiment description |
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells |
Authors |
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ." |
Journal name |
Proteomics
|
Publication year |
2023 |
Sample |
Bone marrow mesenchymal stem cells |
Sample name |
BMSC - Passage 7 |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting Mass spectometry |
|
|
3 |
Experiment ID |
491 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
|
Enriched markers |
✔
Canx
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
36408942
|
Organism |
Rattus norvegicus |
Experiment description |
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells |
Authors |
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ." |
Journal name |
Proteomics
|
Publication year |
2023 |
Sample |
Bone marrow mesenchymal stem cells |
Sample name |
BMSC - Passage 8 |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting Mass spectometry |
|
|
4 |
Experiment ID |
492 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
|
Enriched markers |
✔
Canx
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
36408942
|
Organism |
Rattus norvegicus |
Experiment description |
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells |
Authors |
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ." |
Journal name |
Proteomics
|
Publication year |
2023 |
Sample |
Bone marrow mesenchymal stem cells |
Sample name |
BMSC - Passage 9 |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting Mass spectometry |
|
|
5 |
Experiment ID |
412 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
|
Enriched markers |
✔
CANX|ACTB
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
34108659
|
Organism |
Homo sapiens |
Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
Journal name |
Nat Cell Biol
|
Publication year |
2021 |
Sample |
Breast cancer cells |
Sample name |
MCF7 |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein mRNA |
Methods used in the study |
RT-qPCR Western blotting Mass spectrometry Flow cytometry |
|
|
6 |
Experiment ID |
414 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
|
Enriched markers |
✔
CANX
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
34108659
|
Organism |
Homo sapiens |
Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
Journal name |
Nat Cell Biol
|
Publication year |
2021 |
Sample |
Breast cancer cells |
Sample name |
MDA-MB-231 |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein mRNA |
Methods used in the study |
RT-qPCR Western blotting Mass spectrometry Flow cytometry |
|
|
7 |
Experiment ID |
426 |
MISEV standards |
✘
|
Biophysical techniques |
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
|
Enriched markers |
✔
CANX
|
Negative markers |
✘
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
34108659
|
Organism |
Homo sapiens |
Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
Journal name |
Nat Cell Biol
|
Publication year |
2021 |
Sample |
Breast cancer cells |
Sample name |
MDA-MB-231 - Exo-rich fractions 7-10 pooled |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting Mass spectrometry |
|
|
8 |
Experiment ID |
427 |
MISEV standards |
✘
|
Biophysical techniques |
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
|
Enriched markers |
✔
CANX
|
Negative markers |
✘
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
34108659
|
Organism |
Homo sapiens |
Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
Journal name |
Nat Cell Biol
|
Publication year |
2021 |
Sample |
Breast cancer cells |
Sample name |
MDA-MB-231 - Exo-rich fractions 1-6 pooled |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|
9 |
Experiment ID |
407 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
CD9|CD63|CD81|SDCBP|TSG101|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|RAB35
|
Enriched markers |
✔
CANX
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
34108659
|
Organism |
Homo sapiens |
Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
Journal name |
Nat Cell Biol
|
Publication year |
2021 |
Sample |
Embryonic kidney cells |
Sample name |
HEK293T |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein mRNA |
Methods used in the study |
RT-qPCR Western blotting Mass spectrometry Flow cytometry |
|
|
10 |
Experiment ID |
419 |
MISEV standards |
✘
|
Biophysical techniques |
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
|
Enriched markers |
✔
CANX
|
Negative markers |
✘
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
34108659
|
Organism |
Homo sapiens |
Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
Journal name |
Nat Cell Biol
|
Publication year |
2021 |
Sample |
Embryonic kidney cells |
Sample name |
HEK293T - Exo-rich fractions 7-10 pooled |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting Mass spectrometry |
|
|
11 |
Experiment ID |
419 |
MISEV standards |
✘
|
Biophysical techniques |
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
|
Enriched markers |
✔
CANX
|
Negative markers |
✘
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
34108659
|
Organism |
Homo sapiens |
Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
Journal name |
Nat Cell Biol
|
Publication year |
2021 |
Sample |
Embryonic kidney cells |
Sample name |
HEK293T - Exo-rich fractions 7-10 pooled |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting Mass spectrometry |
|
|
12 |
Experiment ID |
420 |
MISEV standards |
✘
|
Biophysical techniques |
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
|
Enriched markers |
✔
CANX
|
Negative markers |
✘
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
34108659
|
Organism |
Homo sapiens |
Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
Journal name |
Nat Cell Biol
|
Publication year |
2021 |
Sample |
Embryonic kidney cells |
Sample name |
HEK293T - Exo-rich fractions 1-6 pooled |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|
13 |
Experiment ID |
420 |
MISEV standards |
✘
|
Biophysical techniques |
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
|
Enriched markers |
✔
CANX
|
Negative markers |
✘
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
34108659
|
Organism |
Homo sapiens |
Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
Journal name |
Nat Cell Biol
|
Publication year |
2021 |
Sample |
Embryonic kidney cells |
Sample name |
HEK293T - Exo-rich fractions 1-6 pooled |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|
14 |
Experiment ID |
405 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
CD9|CD63|CD81|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
|
Enriched markers |
✔
CANX
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
34108659
|
Organism |
Homo sapiens |
Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
Journal name |
Nat Cell Biol
|
Publication year |
2021 |
Sample |
Foreskin fibroblasts |
Sample name |
BJ |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein mRNA |
Methods used in the study |
RT-qPCR Western blotting Mass spectrometry Flow cytometry |
|
|
15 |
Experiment ID |
417 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
|
Enriched markers |
✔
CANX|ACTB
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
34108659
|
Organism |
Homo sapiens |
Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
Journal name |
Nat Cell Biol
|
Publication year |
2021 |
Sample |
Lymphoma cells |
Sample name |
Raji |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein mRNA |
Methods used in the study |
RT-qPCR Western blotting Mass spectrometry Flow cytometry |
|
|
16 |
Experiment ID |
411 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
|
Enriched markers |
✔
CANX
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
34108659
|
Organism |
Homo sapiens |
Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
Journal name |
Nat Cell Biol
|
Publication year |
2021 |
Sample |
Mammary cancer-associated fibroblasts |
Sample name |
mCAF |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein mRNA |
Methods used in the study |
RT-qPCR Western blotting Mass spectrometry Flow cytometry |
|
|
17 |
Experiment ID |
488 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
|
Enriched markers |
✔
CANX
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
36408942
|
Organism |
Homo sapiens |
Experiment description |
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells |
Authors |
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ." |
Journal name |
Proteomics
|
Publication year |
2023 |
Sample |
Mesenchymal stem cells |
Sample name |
UCMSC |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting Mass spectometry |
|
|
18 |
Experiment ID |
418 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
|
Enriched markers |
✔
CANX
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
34108659
|
Organism |
Homo sapiens |
Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
Journal name |
Nat Cell Biol
|
Publication year |
2021 |
Sample |
Monocytic leukemia cells |
Sample name |
THP-1 |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein mRNA |
Methods used in the study |
RT-qPCR Western blotting Mass spectrometry Flow cytometry |
|
|
19 |
Experiment ID |
418 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
|
Enriched markers |
✔
CANX
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
34108659
|
Organism |
Homo sapiens |
Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
Journal name |
Nat Cell Biol
|
Publication year |
2021 |
Sample |
Monocytic leukemia cells |
Sample name |
THP-1 |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein mRNA |
Methods used in the study |
RT-qPCR Western blotting Mass spectrometry Flow cytometry |
|
|
20 |
Experiment ID |
413 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
|
Enriched markers |
✔
CANX
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
34108659
|
Organism |
Homo sapiens |
Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
Journal name |
Nat Cell Biol
|
Publication year |
2021 |
Sample |
Normal mammary epithelial cells |
Sample name |
MCF10A |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein mRNA |
Methods used in the study |
RT-qPCR Western blotting Mass spectrometry Flow cytometry |
|
|
21 |
Experiment ID |
406 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35|CD81
|
Enriched markers |
✔
CANX
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
34108659
|
Organism |
Homo sapiens |
Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
Journal name |
Nat Cell Biol
|
Publication year |
2021 |
Sample |
Pancreatic cancer cells |
Sample name |
BxPC3 |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein mRNA |
Methods used in the study |
RT-qPCR Western blotting Mass spectrometry Flow cytometry |
|
|
22 |
Experiment ID |
415 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
|
Enriched markers |
✔
CANX
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
34108659
|
Organism |
Homo sapiens |
Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
Journal name |
Nat Cell Biol
|
Publication year |
2021 |
Sample |
Pancreatic cancer cells |
Sample name |
PANC-1 |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein mRNA |
Methods used in the study |
RT-qPCR Western blotting Mass spectrometry Flow cytometry |
|
|
23 |
Experiment ID |
434 |
MISEV standards |
✘
|
Biophysical techniques |
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
|
Enriched markers |
✔
CANX
|
Negative markers |
✘
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
34108659
|
Organism |
Homo sapiens |
Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
Journal name |
Nat Cell Biol
|
Publication year |
2021 |
Sample |
Pancreatic cancer cells |
Sample name |
PANC-1 - Exo-rich fractions 7-10 pooled |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting Mass spectrometry |
|
|
24 |
Experiment ID |
435 |
MISEV standards |
✘
|
Biophysical techniques |
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
|
Enriched markers |
✔
CANX
|
Negative markers |
✘
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
34108659
|
Organism |
Homo sapiens |
Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
Journal name |
Nat Cell Biol
|
Publication year |
2021 |
Sample |
Pancreatic cancer cells |
Sample name |
PANC-1 - Exo-rich fractions 1-6 pooled |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|
25 |
Experiment ID |
408 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
CD9|CD63|CD81|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
|
Enriched markers |
✔
CANX|ACTB
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
34108659
|
Organism |
Homo sapiens |
Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
Journal name |
Nat Cell Biol
|
Publication year |
2021 |
Sample |
Pancreatic duct epithalial cells |
Sample name |
HPDE |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein mRNA |
Methods used in the study |
RT-qPCR Western blotting Mass spectrometry Flow cytometry |
|
|
26 |
Experiment ID |
409 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
|
Enriched markers |
✔
CANX
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
34108659
|
Organism |
Homo sapiens |
Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
Journal name |
Nat Cell Biol
|
Publication year |
2021 |
Sample |
Pancreatic duct epithalial cells |
Sample name |
HPNE |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein mRNA |
Methods used in the study |
RT-qPCR Western blotting Mass spectrometry Flow cytometry |
|
|
27 |
Experiment ID |
416 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
|
Enriched markers |
✔
CANX
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
34108659
|
Organism |
Homo sapiens |
Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
Journal name |
Nat Cell Biol
|
Publication year |
2021 |
Sample |
Pluripotent stem cells |
Sample name |
PSC |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein mRNA |
Methods used in the study |
RT-qPCR Western blotting Mass spectrometry Flow cytometry |
|
|
28 |
Experiment ID |
410 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
|
Enriched markers |
✔
CANX
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
34108659
|
Organism |
Homo sapiens |
Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
Journal name |
Nat Cell Biol
|
Publication year |
2021 |
Sample |
T lymphocytes |
Sample name |
Jurkat |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein mRNA |
Methods used in the study |
RT-qPCR Western blotting Mass spectrometry Flow cytometry |
|
|
Protein-protein interactions for PMPCA |
|
Protein Interactor |
ExoCarta ID |
Identification method |
PubMed |
Species |
1 |
MPST |
4357 |
Co-fractionation |
 |
Homo sapiens |
|
2 |
OXCT1 |
5019 |
Proximity Label-MS |
 |
Homo sapiens |
|
3 |
SKIV2L |
6499 |
Two-hybrid |
 |
Homo sapiens |
|
4 |
GUK1 |
2987 |
Co-fractionation |
 |
Homo sapiens |
|
5 |
MTG2 |
|
Affinity Capture-MS |
 |
Homo sapiens |
Proximity Label-MS |
 |
Homo sapiens |
|
6 |
MRPL24 |
|
Proximity Label-MS |
 |
Homo sapiens |
|
7 |
MRPL27 |
51264 |
Proximity Label-MS |
 |
Homo sapiens |
|
8 |
TXNDC12 |
51060 |
Co-fractionation |
 |
Homo sapiens |
|
9 |
MRPS18C |
|
Proximity Label-MS |
 |
Homo sapiens |
|
10 |
IARS2 |
55699 |
Proximity Label-MS |
 |
Homo sapiens |
|
11 |
CCS |
9973 |
Co-fractionation |
 |
Homo sapiens |
|
12 |
NIT1 |
4817 |
Affinity Capture-MS |
 |
Homo sapiens |
|
13 |
PPM1K |
|
Affinity Capture-MS |
 |
Homo sapiens |
|
14 |
ETFA |
2108 |
Proximity Label-MS |
 |
Homo sapiens |
|
15 |
FXN |
|
Co-fractionation |
 |
Homo sapiens |
|
16 |
POLRMT |
5442 |
Proximity Label-MS |
 |
Homo sapiens |
|
17 |
DNAJC8 |
22826 |
Affinity Capture-MS |
 |
Homo sapiens |
Affinity Capture-MS |
 |
Homo sapiens |
|
18 |
IVD |
3712 |
Affinity Capture-MS |
 |
Homo sapiens |
Proximity Label-MS |
 |
Homo sapiens |
|
19 |
SLC30A9 |
10463 |
Proximity Label-MS |
 |
Homo sapiens |
|
20 |
BCS1L |
617 |
Proximity Label-MS |
 |
Homo sapiens |
|
21 |
PREPL |
9581 |
Affinity Capture-MS |
 |
Homo sapiens |
Proximity Label-MS |
 |
Homo sapiens |
|
22 |
VWA8 |
23078 |
Proximity Label-MS |
 |
Homo sapiens |
|
23 |
MRPL47 |
57129 |
Proximity Label-MS |
 |
Homo sapiens |
|
24 |
KIF14 |
9928 |
Affinity Capture-MS |
 |
Homo sapiens |
|
25 |
ACP6 |
51205 |
Affinity Capture-MS |
 |
Homo sapiens |
|
26 |
RBMX |
27316 |
Proximity Label-MS |
 |
Homo sapiens |
|
27 |
ACADVL |
37 |
Proximity Label-MS |
 |
Homo sapiens |
|
28 |
NT5DC2 |
64943 |
Proximity Label-MS |
 |
Homo sapiens |
|
29 |
TUFM |
7284 |
Proximity Label-MS |
 |
Homo sapiens |
|
30 |
KDELC2 |
143888 |
Co-fractionation |
 |
Homo sapiens |
|
31 |
OGT |
8473 |
Reconstituted Complex |
 |
Homo sapiens |
|
32 |
MRPS6 |
|
Proximity Label-MS |
 |
Homo sapiens |
|
33 |
THG1L |
54974 |
Proximity Label-MS |
 |
Homo sapiens |
|
34 |
DBT |
1629 |
Proximity Label-MS |
 |
Homo sapiens |
|
35 |
PAK7 |
|
Affinity Capture-MS |
 |
Homo sapiens |
|
36 |
MTRF1L |
|
Proximity Label-MS |
 |
Homo sapiens |
|
37 |
ACN9 |
|
Affinity Capture-MS |
 |
Homo sapiens |
Proximity Label-MS |
 |
Homo sapiens |
|
38 |
C17orf102 |
|
Affinity Capture-MS |
 |
Homo sapiens |
|
39 |
MRPL41 |
64975 |
Proximity Label-MS |
 |
Homo sapiens |
|
40 |
ETHE1 |
23474 |
Co-fractionation |
 |
Homo sapiens |
|
41 |
MRPL14 |
64928 |
Proximity Label-MS |
 |
Homo sapiens |
|
42 |
WBSCR16 |
|
Affinity Capture-MS |
 |
Homo sapiens |
|
43 |
HADHA |
3030 |
Proximity Label-MS |
 |
Homo sapiens |
|
44 |
MTIF2 |
4528 |
Proximity Label-MS |
 |
Homo sapiens |
|
45 |
MCCC2 |
64087 |
Affinity Capture-MS |
 |
Homo sapiens |
|
46 |
SDHA |
6389 |
Proximity Label-MS |
 |
Homo sapiens |
|
47 |
KRT86 |
|
Affinity Capture-MS |
 |
Homo sapiens |
|
48 |
ANXA1 |
301 |
Co-fractionation |
 |
Homo sapiens |
|
49 |
MRPL16 |
|
Proximity Label-MS |
 |
Homo sapiens |
|
50 |
CUL3 |
8452 |
Affinity Capture-MS |
 |
Homo sapiens |
|
51 |
DES |
1674 |
Affinity Capture-MS |
 |
Homo sapiens |
|
52 |
GADD45GIP1 |
|
Proximity Label-MS |
 |
Homo sapiens |
|
53 |
VARS2 |
|
Proximity Label-MS |
 |
Homo sapiens |
|
54 |
HNRNPR |
10236 |
Proximity Label-MS |
 |
Homo sapiens |
|
55 |
MTG1 |
|
Proximity Label-MS |
 |
Homo sapiens |
|
56 |
PTPN7 |
5778 |
Affinity Capture-MS |
 |
Homo sapiens |
|
57 |
DLST |
1743 |
Proximity Label-MS |
 |
Homo sapiens |
|
58 |
MRPL3 |
|
Proximity Label-MS |
 |
Homo sapiens |
|
59 |
HIST1H1A |
3024 |
Affinity Capture-MS |
 |
Homo sapiens |
|
60 |
TBCA |
6902 |
Co-fractionation |
 |
Homo sapiens |
|
61 |
COX2 |
4513 |
Proximity Label-MS |
 |
Homo sapiens |
|
62 |
DLAT |
1737 |
Proximity Label-MS |
 |
Homo sapiens |
|
63 |
PDP1 |
54704 |
Affinity Capture-MS |
 |
Homo sapiens |
|
64 |
MRPL18 |
29074 |
Proximity Label-MS |
 |
Homo sapiens |
|
65 |
HSPE1 |
3336 |
Proximity Label-MS |
 |
Homo sapiens |
|
66 |
GLS |
2744 |
Proximity Label-MS |
 |
Homo sapiens |
|
67 |
ATP5J |
522 |
Proximity Label-MS |
 |
Homo sapiens |
|
68 |
CLPX |
10845 |
Proximity Label-MS |
 |
Homo sapiens |
|
69 |
MTPN |
136319 |
Co-fractionation |
 |
Homo sapiens |
|
70 |
COQ5 |
|
Proximity Label-MS |
 |
Homo sapiens |
|
71 |
SUCLG2 |
8801 |
Co-fractionation |
 |
| | | |