Gene description for C12orf10
Gene name chromosome 12 open reading frame 10
Gene symbol C12orf10
Other names/aliases Gamm1
MST024
MSTP024
MYG
MYG1
Species Homo sapiens
 Database cross references - C12orf10
ExoCarta ExoCarta_60314
Vesiclepedia VP_60314
Entrez Gene 60314
HGNC 17590
MIM 611366
 C12orf10 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Neuroblastoma cells 26022510    
Neuroblastoma cells 26022510    
Squamous carcinoma cells 20124223    
 Gene ontology annotations for C12orf10
Molecular Function
    3'-5'-RNA exonuclease activity GO:0000175 IDA
    protein binding GO:0005515 IPI
Biological Process
    mitochondrial RNA metabolic process GO:0000959 IMP
    rRNA processing GO:0006364 IMP
    mRNA processing GO:0006397 IMP
    locomotory exploration behavior GO:0035641 IEA
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 IDA
    nucleolus GO:0005730 IDA
    cytoplasm GO:0005737 IBA
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IDA
    mitochondrial matrix GO:0005759 IDA
 Experiment description of studies that identified C12orf10 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 207
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 208
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 209
MISEV standards
✘ Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 239
MISEV standards
✘ Biophysical techniques
✔
Alix|TSG101|CD9
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 26022510    
Organism Homo sapiens
Experiment description Serum-free culture alters the quantity and protein composition of neuroblastoma-derived extracellular vesicles
Authors "Li J, Lee Y, Johansson HJ, Mager I, Vader P, Nordin JZ, Wiklander OP, Lehtio J, Wood MJ6, Andaloussi SE."
Journal name J Extracell Vesicles
Publication year 2015
Sample Neuroblastoma cells
Sample name N2a OptiMEM - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density 1.10-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 242
MISEV standards
✘ Biophysical techniques
✔
Alix|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 26022510    
Organism Homo sapiens
Experiment description Serum-free culture alters the quantity and protein composition of neuroblastoma-derived extracellular vesicles
Authors "Li J, Lee Y, Johansson HJ, Mager I, Vader P, Nordin JZ, Wiklander OP, Lehtio J, Wood MJ6, Andaloussi SE."
Journal name J Extracell Vesicles
Publication year 2015
Sample Neuroblastoma cells
Sample name N2a Pre-spun - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density 1.10-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
10
Experiment ID 191
MISEV standards
✘ Biophysical techniques
✔
Alix|CD81|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors "Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK."
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for C12orf10
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SFT2D1 113402
Two-hybrid Homo sapiens
2 HORMAD1  
Two-hybrid Homo sapiens
3 AHSA1 10598
Co-fractionation Homo sapiens
4 TMEM239  
Two-hybrid Homo sapiens
5 NOTCH2NL 388677
Affinity Capture-MS Homo sapiens
6 CDS2 8760
Two-hybrid Homo sapiens
7 ALDH7A1 501
Two-hybrid Homo sapiens
8 OXCT1 5019
Co-fractionation Homo sapiens
9 GALT 2592
Affinity Capture-MS Homo sapiens
10 IDH1 3417
Co-fractionation Homo sapiens
11 GRB2 2885
Two-hybrid Homo sapiens
12 DCAF10  
Affinity Capture-MS Homo sapiens
13 SLC17A9  
Two-hybrid Homo sapiens
14 HSPA8 3312
Co-fractionation Homo sapiens
15 WWOX 51741
Two-hybrid Homo sapiens
16 SUGT1 10910
Co-fractionation Homo sapiens
17 UBQLN2 29978
Co-fractionation Homo sapiens
18 SYP  
Two-hybrid Homo sapiens
19 FAM9B  
Two-hybrid Homo sapiens
20 RBBP7 5931
Co-fractionation Homo sapiens
21 METTL9 51108
Two-hybrid Homo sapiens
22 UBQLN1 29979
Co-fractionation Homo sapiens
23 DESI2  
Two-hybrid Homo sapiens
24 HSP90AB1 3326
Affinity Capture-MS Homo sapiens
25 HSPB1 3315
Two-hybrid Homo sapiens
26 ELAC2 60528
Co-fractionation Homo sapiens
27 GADD45GIP1  
Two-hybrid Homo sapiens
28 HSP90AA1 3320
Affinity Capture-MS Homo sapiens
29 ARL6IP1 23204
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
30 MAN1C1 57134
Two-hybrid Homo sapiens
31 DDX58 23586
Affinity Capture-RNA Homo sapiens
32 APOA5 116519
Two-hybrid Homo sapiens
33 AGTRAP 57085
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
34 DDX39A 10212
Co-fractionation Homo sapiens
35 CPLX2 10814
Two-hybrid Homo sapiens
36 BSND  
Two-hybrid Homo sapiens
37 TMEM229B  
Two-hybrid Homo sapiens
38 RPA3 6119
Proximity Label-MS Homo sapiens
39 CMTM5  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
40 PLIN3 10226
Two-hybrid Homo sapiens
41 COPS5 10987
Two-hybrid Homo sapiens
42 DPH5 51611
Co-fractionation Homo sapiens
43 DSTN 11034
Co-fractionation Homo sapiens
44 GLRX3 10539
Co-fractionation Homo sapiens
45 RXRB 6257
Two-hybrid Homo sapiens
46 MMAB 326625
Affinity Capture-MS Homo sapiens
47 HIBCH 26275
Co-fractionation Homo sapiens
48 DNAJC17  
Co-fractionation Homo sapiens
49 TMEM56  
Two-hybrid Homo sapiens
50 DDIT4L  
Two-hybrid Homo sapiens
51 TKFC 26007
Co-fractionation Homo sapiens
52 HSPD1 3329
Co-fractionation Homo sapiens
53 SORD 6652
Co-fractionation Homo sapiens
54 DGAT2L6  
Two-hybrid Homo sapiens
55 DDX39B 7919
Co-fractionation Homo sapiens
56 YIF1A 10897
Two-hybrid Homo sapiens
57 LHPP 64077
Affinity Capture-MS Homo sapiens
58 PGD 5226
Co-fractionation Homo sapiens
59 XPNPEP1 7511
Co-fractionation Homo sapiens
60 THOP1 7064
Co-fractionation Homo sapiens
View the network image/svg+xml
 Pathways in which C12orf10 is involved
No pathways found





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