Experiment description of studies that identified HEXIM1 in exosomes

1 Experiment ID 426 MISEV standards ✘ Biophysical techniques ✔ SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1 Enriched markers ✔ CANX Negative markers ✘ Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 34108659     Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." Journal name Nat Cell Biol Publication year 2021 Sample Breast cancer cells Sample name MDA-MB-231 - Exo-rich fractions 7-10 pooled Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

2 Experiment ID 427 MISEV standards ✘ Biophysical techniques ✔ CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1 Enriched markers ✔ CANX Negative markers ✘ Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 34108659     Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." Journal name Nat Cell Biol Publication year 2021 Sample Breast cancer cells Sample name MDA-MB-231 - Exo-rich fractions 1-6 pooled Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

3 Experiment ID 419 MISEV standards ✘ Biophysical techniques ✔ SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1 Enriched markers ✔ CANX Negative markers ✘ Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 34108659     Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." Journal name Nat Cell Biol Publication year 2021 Sample Embryonic kidney cells Sample name HEK293T - Exo-rich fractions 7-10 pooled Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

4 Experiment ID 419 MISEV standards ✘ Biophysical techniques ✔ SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1 Enriched markers ✔ CANX Negative markers ✘ Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 34108659     Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." Journal name Nat Cell Biol Publication year 2021 Sample Embryonic kidney cells Sample name HEK293T - Exo-rich fractions 7-10 pooled Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

5 Experiment ID 420 MISEV standards ✘ Biophysical techniques ✔ CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1 Enriched markers ✔ CANX Negative markers ✘ Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 34108659     Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." Journal name Nat Cell Biol Publication year 2021 Sample Embryonic kidney cells Sample name HEK293T - Exo-rich fractions 1-6 pooled Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

6 Experiment ID 420 MISEV standards ✘ Biophysical techniques ✔ CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1 Enriched markers ✔ CANX Negative markers ✘ Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 34108659     Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." Journal name Nat Cell Biol Publication year 2021 Sample Embryonic kidney cells Sample name HEK293T - Exo-rich fractions 1-6 pooled Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

7 Experiment ID 235 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|Alix|HSC70|GAPDH Enriched markers ✔ HSP90B1 Negative markers ✔ qNano Particle analysis Identified molecule mRNA Identification method RNA Sequencing PubMed ID 26054723     Organism Homo sapiens Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N" Journal name Carcinogenesis Publication year 2015 Sample Hepatocellular carcinoma cells Sample name HKCI-8 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.13-1.19 g/mL Molecules identified in the study Protein RNA Methods used in the study Western blotting Mass spectrometry RT-PCR RNA Sequencing

8 Experiment ID 237 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|Alix|HSC70|GAPDH Enriched markers ✔ HSP90B1 Negative markers ✔ qNano Particle analysis Identified molecule mRNA Identification method RNA Sequencing PubMed ID 26054723     Organism Homo sapiens Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N" Journal name Carcinogenesis Publication year 2015 Sample Hepatocytes Sample name MIHA Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.13-1.19 g/mL Molecules identified in the study Protein RNA Methods used in the study Western blotting Mass spectrometry RNA Sequencing

9 Experiment ID 434 MISEV standards ✘ Biophysical techniques ✔ SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1 Enriched markers ✔ CANX Negative markers ✘ Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 34108659     Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." Journal name Nat Cell Biol Publication year 2021 Sample Pancreatic cancer cells Sample name PANC-1 - Exo-rich fractions 7-10 pooled Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

10 Experiment ID 435 MISEV standards ✘ Biophysical techniques ✔ CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1 Enriched markers ✔ CANX Negative markers ✘ Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 34108659     Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." Journal name Nat Cell Biol Publication year 2021 Sample Pancreatic cancer cells Sample name PANC-1 - Exo-rich fractions 1-6 pooled Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

Protein-protein interactions for HEXIM1

Protein Interactor ExoCarta ID Identification method PubMed Species 1 KLHL9 55958 Affinity Capture-MS Homo sapiens 2 RPSA 3921 Affinity Capture-MS Homo sapiens 3 RIT1 6016 Negative Genetic Homo sapiens 4 HNRNPH1 3187 Affinity Capture-RNA Homo sapiens 5 PPP1CB 5500 Affinity Capture-MS Homo sapiens 6 PPIL4   Affinity Capture-MS Homo sapiens 7 RPS19 6223 Affinity Capture-MS Homo sapiens 8 CSNK2A1 1457 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 9 AFF1   Affinity Capture-MS Homo sapiens Affinity Capture-Western Homo sapiens Affinity Capture-Western Homo sapiens Affinity Capture-Western Homo sapiens 10 PPP1R10   Affinity Capture-MS Homo sapiens 11 PRKDC 5591 Co-fractionation Homo sapiens 12 RPS3 6188 Affinity Capture-MS Homo sapiens 13 SURF2   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 14 RNPS1 10921 Affinity Capture-MS Homo sapiens 15 DYRK1A 1859 Affinity Capture-MS Homo sapiens 16 AFF4 27125 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 17 UBE2O 63893 Affinity Capture-MS Homo sapiens 18 ATXN2L 11273 Affinity Capture-MS Homo sapiens 19 SHMT2 6472 Affinity Capture-RNA Homo sapiens 20 CPSF7 79869 Affinity Capture-MS Homo sapiens 21 H2AFY2 55506 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 22 DDOST 1650 Affinity Capture-MS Homo sapiens 23 TULP3 7289 Affinity Capture-MS Homo sapiens 24 BRD4 23476 Affinity Capture-Western Homo sapiens 25 NUSAP1 51203 Affinity Capture-MS Homo sapiens 26 ATG16L1 55054 Affinity Capture-MS Homo sapiens 27 DDX6 1656 Affinity Capture-MS Homo sapiens 28 MECP2 4204 Affinity Capture-MS Homo sapiens 29 CSNK1D 1453 Affinity Capture-MS Homo sapiens 30 CSNK2B 1460 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 31 TNFRSF1B   Affinity Capture-MS Homo sapiens 32 AMOT   Affinity Capture-MS Homo sapiens 33 DYNC1H1 1778 Co-fractionation Homo sapiens 34 POLA2   Co-fractionation Homo sapiens 35 E2F4   Affinity Capture-MS Homo sapiens 36 POP1 10940 Affinity Capture-MS Homo sapiens 37 ACTA2 59 Affinity Capture-MS Homo sapiens 38 HAUS2   Affinity Capture-MS Homo sapiens 39 RN7SK 125050 Affinity Capture-Western Homo sapiens Affinity Capture-RNA Homo sapiens Affinity Capture-Western Homo sapiens 40 DDX21 9188 Affinity Capture-MS Homo sapiens 41 MEPCE 56257 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 42 NONO 4841 Affinity Capture-MS Homo sapiens 43 CLTCL1 8218 Affinity Capture-MS Homo sapiens 44 SRSF6 6431 Affinity Capture-MS Homo sapiens Co-fractionation Homo sapiens 45 Vav2   Affinity Capture-MS Mus musculus 46 AKR7L 246181 Affinity Capture-MS Homo sapiens 47 DEK 7913 Affinity Capture-MS Homo sapiens 48 OSBPL1A 114876 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 49 SREBF2 6721 Negative Genetic Homo sapiens 50 XRCC5 7520 Co-fractionation Homo sapiens 51 TOX4   Affinity Capture-MS Homo sapiens 52 DDX1 1653 Co-fractionation Homo sapiens 53 DHX9 1660 Affinity Capture-Western Homo sapiens 54 COPS7B 64708 Co-fractionation Homo sapiens 55 PPM1G 5496 Reconstituted Complex Homo sapiens Reconstituted Complex Homo sapiens 56 CEBPA   Protein-peptide Homo sapiens 57 CCNT1   Affinity Capture-MS Homo sapiens Two-hybrid Homo sapiens Affinity Capture-Western Homo sapiens Affinity Capture-Western Homo sapiens Affinity Capture-Western Homo sapiens Reconstituted Complex Homo sapiens Affinity Capture-Western Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Reconstituted Complex Homo sapiens 58 WDR82 80335 Affinity Capture-MS Homo sapiens 59 SRSF11 9295 Co-fractionation Homo sapiens 60 SFPQ 6421 Affinity Capture-MS Homo sapiens 61 Terf2   Affinity Capture-MS Mus musculus 62 EGLN3   Affinity Capture-Western Homo sapiens 63 RTN4IP1   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 64 AP1M1 8907 Co-fractionation Homo sapiens 65 ATP5F1 515 Affinity Capture-MS Homo sapiens 66 CTTN 2017 Co-fractionation Homo sapiens Affinity Capture-MS Homo sapiens 67 EGFR 1956 PCA Homo sapiens 68 YY1 7528 Affinity Capture-MS Homo sapiens 69 CCDC71   Affinity Capture-MS Homo sapiens 70 SART1 9092 Affinity Capture-MS Homo sapiens 71 KLHL13 90293 Affinity Capture-MS Homo sapiens 72 NXF1 10482 Affinity Capture-RNA Homo sapiens 73 NCAPH 23397 Affinity Capture-MS Homo sapiens 74 DDX42 11325 Affinity Capture-MS Homo sapiens 75 ACTR5 79913 Affinity Capture-MS Homo sapiens 76 PSPC1 55269 Affinity Capture-MS Homo sapiens 77 RPA3 6119 Proximity Label-MS Homo sapiens 78 CSNK2A2 1459 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 79 TOP1 7150 Affinity Capture-MS Homo sapiens 80 EEF1A2 1917 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 81 NDC80 10403 Affinity Capture-MS Homo sapiens 82 MATR3 9782 Co-fractionation Homo sapiens 83 Rpl35 66489 Affinity Capture-MS Mus musculus 84 HSPD1 3329 Co-fractionation Homo sapiens 85 AAMDC   Affinity Capture-MS Homo sapiens 86 LMNA 4000 Affinity Capture-MS Homo sapiens 87 FHL2 2274 Co-fractionation Homo sapiens 88 FTSJ3 117246 Affinity Capture-MS Homo sapiens 89 NSMAF   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 90 MDM2   Affinity Capture-Western Homo sapiens 91 DDX55   Affinity Capture-MS Homo sapiens 92 C10orf2 56652 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 93 HIF1A 3091 Affinity Capture-Western Homo sapiens 94 RELA 5970 Reconstituted Complex Homo sapiens 95 EIF1AD 84285 Affinity Capture-MS Homo sapiens 96 NUDT21 11051 Affinity Capture-MS Homo sapiens 97 PLEKHA4 57664 Affinity Capture-MS Homo sapiens 98 TERF2   Two-hybrid Homo sapiens 99 TP53 7157 Affinity Capture-Western Homo sapiens Affinity Capture-Western Homo sapiens 100 XRCC6 2547 Co-fractionation Homo sapiens 101 NUFIP2 57532 Affinity Capture-MS Homo sapiens 102 UBA52 7311 Affinity Capture-MS Homo sapiens 103 SH3BP5L   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 104 C1orf35   Affinity Capture-MS Homo sapiens 105 HDAC1 3065 Affinity Capture-Western Homo sapiens 106 TAF2   Affinity Capture-MS Homo sapiens 107 ESR1   Co-localization Homo sapiens Affinity Capture-Western Homo sapiens Reconstituted Complex Homo sapiens 108 LARP7 51574 Affinity Capture-Western Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-Western Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Co-fractionation Homo sapiens 109 TERF1 7013 Two-hybrid Homo sapiens 110 HEXIM2   Affinity Capture-Western Homo sapiens Two-hybrid Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 111 MOV10 4343 Affinity Capture-RNA Homo sapiens 112 AURKA 6790 Affinity Capture-MS Homo sapiens 113 CDK9 1025 Affinity Capture-Western Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-Western Homo sapiens Reconstituted Complex Homo sapiens Affinity Capture-Western Homo sapiens Affinity Capture-Western Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-Western Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-Western Homo sapiens Affinity Capture-Western Homo sapiens Affinity Capture-Western Homo sapiens Affinity Capture-Western Homo sapiens Affinity Capture-Western Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Reconstituted Complex Homo sapiens 114 SLC9A3R1 9368 Affinity Capture-MS Homo sapiens 115 RPL8 6132 Affinity Capture-MS Homo sapiens 116 CCNT2   Two-hybrid Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 117 EPHA2 1969 Affinity Capture-MS Homo sapiens 118 LDLR 3949 Negative Genetic Homo sapiens 119 EIF5A 1984 Affinity Capture-MS Homo sapiens 120 RPL23A 6147 Affinity Capture-MS Homo sapiens 121 AR 367 Proximity Label-MS Homo sapiens 122 METTL17   Affinity Capture-MS Homo sapiens 123 NUCKS1 64710 Co-fractionation Homo sapiens 124 GPKOW   Affinity Capture-MS Homo sapiens View the network

Pathways in which HEXIM1 is involved

No pathways found