Gene description for SNRPE
Gene name small nuclear ribonucleoprotein polypeptide E
Gene symbol SNRPE
Other names/aliases B-raf
HYPT11
SME
Sm-E
Species Homo sapiens
 Database cross references - SNRPE
ExoCarta ExoCarta_6635
Entrez Gene 6635
HGNC 11161
MIM 128260
UniProt P62304  
 SNRPE identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 25890246    
Colorectal cancer cells 25890246    
Prostate cancer cells 22723089    
Thymus 23844026    
 Gene ontology annotations for SNRPE
Molecular Function
    RNA binding GO:0003723 IEA
    protein binding GO:0005515 IPI
Biological Process
    RNA splicing GO:0008380 TAS
    hair cycle GO:0042633 IMP
    spliceosomal snRNP assembly GO:0000387 TAS
    spliceosomal complex assembly GO:0000245 NAS
    mRNA splicing, via spliceosome GO:0000398 TAS
    histone mRNA metabolic process GO:0008334 TAS
    mRNA 3'-end processing GO:0031124 TAS
    transcription from RNA polymerase II promoter GO:0006366 TAS
    termination of RNA polymerase II transcription GO:0006369 TAS
    ncRNA metabolic process GO:0034660 TAS
    gene expression GO:0010467 TAS
Subcellular Localization
    SMN-Sm protein complex GO:0034719 IDA
    nucleus GO:0005634 IDA
    U1 snRNP GO:0005685 IDA
    methylosome GO:0034709 IDA
    extracellular exosome GO:0070062 IDA
    precatalytic spliceosome GO:0071011 IBA
    catalytic step 2 spliceosome GO:0071013 IDA
    pICln-Sm protein complex GO:0034715 IDA
    U5 snRNP GO:0005682 IBA
    U4 snRNP GO:0005687 IDA
    U7 snRNP GO:0005683 IDA
    spliceosomal complex GO:0005681 NAS
    nucleoplasm GO:0005654 TAS
    U12-type spliceosomal complex GO:0005689 IDA
    U4/U6 x U5 tri-snRNP complex GO:0046540 IBA
    cytosol GO:0005829 TAS
    U2 snRNP GO:0005686 IBA
    small nuclear ribonucleoprotein complex GO:0030532 NAS
 Experiment description of studies that identified SNRPE in exosomes
1
Experiment ID 282
ISEV standards
CEM
EV Biophysical techniques
Alix|TSG101
EV Cytosolic markers
CD63|CD81|EpCAM
EV Membrane markers
EV Negative markers
DLS
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors Xu R, Greening DW, Rai A, Ji H, Simpson RJ.
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Ultracentrifugation - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 283
ISEV standards
CEM
EV Biophysical techniques
Alix|TSG101
EV Cytosolic markers
CD63|CD81|EpCAM
EV Membrane markers
EV Negative markers
DLS
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors Xu R, Greening DW, Rai A, Ji H, Simpson RJ.
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Ultracentrifugation - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
3
Experiment ID 145
ISEV standards
EM
EV Biophysical techniques
HSP70|HSP90|RAB5
EV Cytosolic markers
CD10
EV Membrane markers
HSP90B1
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method
Mass spectrometry [QTOF]   
PubMed ID 22723089    
Organism Homo sapiens
Experiment description Prostate cancer cell derived exosomes
Authors Hosseini-Beheshti E, Guns ES.
Journal name MCP
Publication year 2012
Sample Prostate cancer cells
Sample name C4-2 - Rep 3
Isolation/purification methods Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
4
Experiment ID 217
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
CD81|CD9|CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for SNRPE
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 YWHAH 7533
Affinity Capture-Western Homo sapiens
2 LSM5 23658
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
3 GEMIN5 25929
Reconstituted Complex Homo sapiens
4 SNRPD3 6634
Two-hybrid Homo sapiens
5 GEMIN6  
Affinity Capture-MS Homo sapiens
6 LSM4  
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
7 SMN1 6606
Reconstituted Complex Homo sapiens
8 VCL 7414
Affinity Capture-MS Homo sapiens
9 SNRPE 6635
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 LSM3 27258
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
11 WDR77 79084
Reconstituted Complex Homo sapiens
12 DDX20  
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
13 LSM2  
Affinity Capture-MS Homo sapiens
14 SNRPG 6637
Invitro Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
15 SNRPF 6636
Invitro Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
16 CLNS1A 1207
Affinity Capture-Western Homo sapiens
17 SNRPD2 6633
Two-hybrid Homo sapiens
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