Gene description for HARS2
Gene name histidyl-tRNA synthetase 2, mitochondrial
Gene symbol HARS2
Other names/aliases HARSL
HARSR
HO3
PRLTS2
Species Homo sapiens
 Database cross references - HARS2
ExoCarta ExoCarta_23438
Vesiclepedia VP_23438
Entrez Gene 23438
HGNC 4817
MIM 600783
UniProt P49590  
 HARS2 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for HARS2
Molecular Function
    RNA binding GO:0003723 HDA
    RNA binding GO:0003723 IBA
    histidine-tRNA ligase activity GO:0004821 IBA
    histidine-tRNA ligase activity GO:0004821 IDA
    histidine-tRNA ligase activity GO:0004821 NAS
    histidine-tRNA ligase activity GO:0004821 TAS
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    identical protein binding GO:0042802 IBA
    identical protein binding GO:0042802 IPI
Biological Process
    translation GO:0006412 NAS
    tRNA aminoacylation for protein translation GO:0006418 TAS
    histidyl-tRNA aminoacylation GO:0006427 IBA
    histidyl-tRNA aminoacylation GO:0006427 IDA
Subcellular Localization
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IBA
    mitochondrion GO:0005739 IDA
    mitochondrial matrix GO:0005759 TAS
    cytosol GO:0005829 IBA
 Experiment description of studies that identified HARS2 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 231
MISEV standards
Biophysical techniques
Alix|CD63|CD9
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 232
MISEV standards
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 233
MISEV standards
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 834
MISEV standards
EM
Biophysical techniques
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 835
MISEV standards
EM
Biophysical techniques
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for HARS2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RPUSD4 84881
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 UQCRFS1 7386
Affinity Capture-MS Homo sapiens
3 PDYN  
Affinity Capture-MS Homo sapiens
4 STAT5B 6777
Affinity Capture-MS Homo sapiens
5 FAM167A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 LAMP3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 HCST  
Affinity Capture-MS Homo sapiens
8 TRUB2  
Proximity Label-MS Homo sapiens
9 MTG2  
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 NDUFA4 4697
Affinity Capture-MS Homo sapiens
11 FAM174A 345757
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 DGAT2  
Affinity Capture-MS Homo sapiens
13 MTRF1  
Proximity Label-MS Homo sapiens
14 DNAJB6 10049
Affinity Capture-MS Homo sapiens
15 LRPPRC 10128
Proximity Label-MS Homo sapiens
16 LRP1 4035
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 IMP3 55272
Affinity Capture-MS Homo sapiens
18 MRFAP1L1  
Affinity Capture-MS Homo sapiens
19 ATP5D 513
Affinity Capture-MS Homo sapiens
20 TFAM 7019
Proximity Label-MS Homo sapiens
21 SARS2 54938
Co-fractionation Homo sapiens
22 SDHB 6390
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 MRPL23 6150
Affinity Capture-MS Homo sapiens
24 C4orf3  
Affinity Capture-MS Homo sapiens
25 HINT2 84681
Proximity Label-MS Homo sapiens
26 STX7 8417
Affinity Capture-MS Homo sapiens
27 TREML2 79865
Affinity Capture-MS Homo sapiens
28 MRPL12 6182
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 NUP210 23225
Affinity Capture-MS Homo sapiens
30 NUDT9 53343
Affinity Capture-MS Homo sapiens
31 PMEL 6490
Affinity Capture-MS Homo sapiens
32 MALSU1  
Affinity Capture-MS Homo sapiens
33 SEC11C 90701
Affinity Capture-MS Homo sapiens
34 NTF3  
Affinity Capture-MS Homo sapiens
35 SCRN2 90507
Co-fractionation Homo sapiens
36 PHF7  
Affinity Capture-MS Homo sapiens
37 MRPS7 51081
Affinity Capture-MS Homo sapiens
38 KLHL17  
Affinity Capture-MS Homo sapiens
39 VPS16 64601
Affinity Capture-MS Homo sapiens
40 PRKCD 5580
Co-fractionation Homo sapiens
41 PMPCB 9512
Proximity Label-MS Homo sapiens
42 FASTKD2  
Proximity Label-MS Homo sapiens
43 SARS 6301
Co-fractionation Homo sapiens
44 HSPD1 3329
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 MTERF3  
Proximity Label-MS Homo sapiens
46 FADS3 3995
Affinity Capture-MS Homo sapiens
47 MYLK4  
Affinity Capture-MS Homo sapiens
48 MDH2 4191
Proximity Label-MS Homo sapiens
49 GOLGA2 2801
Affinity Capture-MS Homo sapiens
50 SMPD2 6610
Affinity Capture-MS Homo sapiens
51 TFDP2  
Affinity Capture-MS Homo sapiens
52 AURKA 6790
Affinity Capture-MS Homo sapiens
53 C1orf64  
Affinity Capture-MS Homo sapiens
54 AGTRAP 57085
Two-hybrid Homo sapiens
55 C6orf203  
Proximity Label-MS Homo sapiens
56 ACSM5 54988
Affinity Capture-MS Homo sapiens
57 PARK2  
Affinity Capture-MS Homo sapiens
58 FADS1 3992
Affinity Capture-MS Homo sapiens
59 AMACR 23600
Affinity Capture-MS Homo sapiens
60 IL1R2  
Affinity Capture-MS Homo sapiens
61 TUFM 7284
Proximity Label-MS Homo sapiens
62 LRRC25  
Affinity Capture-MS Homo sapiens
63 OGT 8473
Reconstituted Complex Homo sapiens
64 GFM1 85476
Proximity Label-MS Homo sapiens
65 ACOT6  
Affinity Capture-MS Homo sapiens
66 MRPL11 65003
Proximity Label-MS Homo sapiens
67 MTRF1L  
Proximity Label-MS Homo sapiens
68 PRKAB1 5564
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
69 HSD17B8  
Affinity Capture-MS Homo sapiens
70 SPRYD7 57213
Affinity Capture-MS Homo sapiens
71 POLG2  
Affinity Capture-MS Homo sapiens
72 ANLN 54443
Affinity Capture-MS Homo sapiens
73 GPIHBP1  
Affinity Capture-MS Homo sapiens
74 GINS2  
Affinity Capture-MS Homo sapiens
75 OTC 5009
Affinity Capture-MS Homo sapiens
76 MRPL21 219927
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
77 MRRF  
Proximity Label-MS Homo sapiens
78 THEM5  
Affinity Capture-MS Homo sapiens
79 C10orf107  
Affinity Capture-MS Homo sapiens
80 MTIF2 4528
Proximity Label-MS Homo sapiens
81 CLPP 8192
Proximity Label-MS Homo sapiens
82 MTIF3  
Proximity Label-MS Homo sapiens
83 LMNB2 84823
Affinity Capture-MS Homo sapiens
84 AKAP13 11214
Affinity Capture-MS Homo sapiens
85 FAHD1 81889
Affinity Capture-MS Homo sapiens
86 HSD17B13  
Affinity Capture-MS Homo sapiens
87 FUS 2521
Affinity Capture-MS Homo sapiens
88 NXF1 10482
Affinity Capture-RNA Homo sapiens
89 TMEM173  
Affinity Capture-MS Homo sapiens
90 GPX8 493869
Affinity Capture-MS Homo sapiens
91 BIRC6 57448
Affinity Capture-MS Homo sapiens
92 NRBP1 29959
Affinity Capture-MS Homo sapiens
93 ORC1  
Affinity Capture-MS Homo sapiens
94 CD79A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
95 RPA3 6119
Proximity Label-MS Homo sapiens
96 DHX30 22907
Proximity Label-MS Homo sapiens
97 EGFR 1956
Negative Genetic Homo sapiens
98 MAGEF1  
Affinity Capture-MS Homo sapiens
99 RPL35A 6165
Affinity Capture-MS Homo sapiens
100 MED23 9439
Co-fractionation Homo sapiens
101 MOV10 4343
Affinity Capture-RNA Homo sapiens
102 TRAC  
Affinity Capture-MS Homo sapiens
103 ICE2  
Affinity Capture-MS Homo sapiens
104 KRAS 3845
Negative Genetic Homo sapiens
105 SEPT11 55752
Affinity Capture-MS Homo sapiens
106 MRPS17 51373
Affinity Capture-MS Homo sapiens
107 FAM217B  
Affinity Capture-MS Homo sapiens
108 PDP1 54704
Affinity Capture-MS Homo sapiens
109 PFDN5 5204
Affinity Capture-MS Homo sapiens
110 GRAMD1B  
Affinity Capture-MS Homo sapiens
111 NCAPG 64151
Affinity Capture-MS Homo sapiens
112 YIF1B 90522
Affinity Capture-MS Homo sapiens
113 CKM  
Affinity Capture-MS Homo sapiens
114 CS 1431
Proximity Label-MS Homo sapiens
115 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
116 TSFM 10102
Proximity Label-MS Homo sapiens
117 AUH 549
Proximity Label-MS Homo sapiens
118 SERINC2 347735
Affinity Capture-MS Homo sapiens
119 ICT1 3396
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
120 OSTM1 28962
Affinity Capture-MS Homo sapiens
121 NUDT13  
Affinity Capture-MS Homo sapiens
122 BST2 684
Two-hybrid Homo sapiens
123 PDHA1 5160
Proximity Label-MS Homo sapiens
124 AGMAT  
Affinity Capture-MS Homo sapiens
125 C12orf65  
Proximity Label-MS Homo sapiens
126 TROAP  
Affinity Capture-MS Homo sapiens
127 NDUFS7 374291
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
128 COX8A  
Proximity Label-MS Homo sapiens
129 GTF2H5  
Affinity Capture-MS Homo sapiens
130 MFSD11  
Affinity Capture-MS Homo sapiens
131 SUCLA2 8803
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
132 SHMT2 6472
Affinity Capture-RNA Homo sapiens
133 C21orf33  
Proximity Label-MS Homo sapiens
134 SSBP1 6742
Proximity Label-MS Homo sapiens
135 GORASP1 64689
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
136 GARS 2617
Co-fractionation Homo sapiens
137 KRR1 11103
Affinity Capture-MS Homo sapiens
138 TXN 7295
Co-fractionation Homo sapiens
139 NAA30 122830
Affinity Capture-MS Homo sapiens
140 PDLIM5 10611
Affinity Capture-MS Homo sapiens
141 TBRG4 9238
Proximity Label-MS Homo sapiens
142 AARS2  
Proximity Label-MS Homo sapiens
143 HARS 3035
Cross-Linking-MS (XL-MS) Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
144 GRSF1 2926
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
145 C1QBP 708
Proximity Label-MS Homo sapiens
146 SERBP1 26135
Affinity Capture-MS Homo sapiens
147 C1orf210  
Affinity Capture-MS Homo sapiens
148 METTL17  
Proximity Label-MS Homo sapiens
149 YBEY  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
150 ALS2CR12  
Affinity Capture-MS Homo sapiens
151 DDX28  
Proximity Label-MS Homo sapiens
152 ACAA2 10449
Affinity Capture-MS Homo sapiens
153 DKKL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which HARS2 is involved
PathwayEvidenceSource
Metabolism of proteins TAS Reactome
Mitochondrial tRNA aminoacylation TAS Reactome
Translation TAS Reactome
tRNA Aminoacylation TAS Reactome





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