Gene description for PIK3R3
Gene name phosphoinositide-3-kinase, regulatory subunit 3 (gamma)
Gene symbol PIK3R3
Other names/aliases p55
p55-GAMMA
p55PIK
Species Homo sapiens
 Database cross references - PIK3R3
ExoCarta ExoCarta_8503
Vesiclepedia VP_8503
Entrez Gene 8503
HGNC 8981
MIM 606076
UniProt Q92569  
 PIK3R3 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for PIK3R3
Molecular Function
    phosphotyrosine residue binding GO:0001784 IPI
    protein binding GO:0005515 IPI
    1-phosphatidylinositol-3-kinase activity GO:0016303 TAS
    1-phosphatidylinositol-3-kinase regulator activity GO:0046935 IBA
Biological Process
    positive regulation of protein phosphorylation GO:0001934 IMP
    cell migration involved in sprouting angiogenesis GO:0002042 IGI
    immune response GO:0006955 NAS
    insulin receptor signaling pathway GO:0008286 IBA
    positive regulation of gene expression GO:0010628 IMP
    B cell differentiation GO:0030183 NAS
    T cell differentiation GO:0030217 NAS
    positive regulation of cell migration GO:0030335 NAS
    phosphatidylinositol-3-phosphate biosynthetic process GO:0036092 IEA
    phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0043491 IMP
    negative regulation of anoikis GO:2000811 NAS
Subcellular Localization
    cytosol GO:0005829 TAS
    phosphatidylinositol 3-kinase complex, class IA GO:0005943 IBA
    phosphatidylinositol 3-kinase complex, class IA GO:0005943 NAS
 Experiment description of studies that identified PIK3R3 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for PIK3R3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SSBP4  
Two-hybrid Homo sapiens
2 ENKD1  
Two-hybrid Homo sapiens
3 KLC2 64837
Two-hybrid Homo sapiens
4 FBN3 84467
Two-hybrid Homo sapiens
5 QARS 5859
Two-hybrid Homo sapiens
6 STAT5B 6777
Two-hybrid Homo sapiens
7 CRK 1398
Affinity Capture-MS Homo sapiens
8 GC 2638
Two-hybrid Homo sapiens
9 TRIM66  
Affinity Capture-MS Homo sapiens
10 RARRES3  
Two-hybrid Homo sapiens
11 PLEKHF2 79666
Two-hybrid Homo sapiens
12 KARS 3735
Two-hybrid Homo sapiens
13 SPATA32  
Two-hybrid Homo sapiens
14 TRIM54  
Two-hybrid Homo sapiens
15 HEPACAM2  
Affinity Capture-MS Homo sapiens
16 PIK3CD 5293
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
17 AMHR2  
Affinity Capture-MS Homo sapiens
18 PBX4  
Two-hybrid Homo sapiens
19 BTN2A1 11120
Affinity Capture-MS Homo sapiens
20 DARS 1615
Two-hybrid Homo sapiens
21 PDGFRB 5159
Co-localization Homo sapiens
22 ORM1 5004
Two-hybrid Homo sapiens
23 FAM166B  
Two-hybrid Homo sapiens
24 YES1 7525
Two-hybrid Homo sapiens
25 KHDRBS1 10657
Two-hybrid Homo sapiens
26 SLC31A1 1317
Affinity Capture-MS Homo sapiens
27 PSME1 5720
Two-hybrid Homo sapiens
28 ACVR2B  
Affinity Capture-MS Homo sapiens
29 ZBTB18  
Two-hybrid Homo sapiens
30 RAB35 11021
Proximity Label-MS Homo sapiens
31 MICAL1 64780
Two-hybrid Homo sapiens
32 ERBB2 2064
Protein-peptide Homo sapiens
Reconstituted Complex Homo sapiens
33 HSPD1 3329
Affinity Capture-MS Homo sapiens
34 SERTAD2 9792
Two-hybrid Homo sapiens
35 RB1 5925
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
36 NEDD9 4739
Two-hybrid Homo sapiens
37 IRS4 8471
Affinity Capture-Luminescence Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 MANSC1 54682
Affinity Capture-MS Homo sapiens
39 KIT 3815
Reconstituted Complex Homo sapiens
40 CX3CL1  
Affinity Capture-MS Homo sapiens
41 CEP19  
Two-hybrid Homo sapiens
42 PIBF1  
Affinity Capture-MS Homo sapiens
43 IGF1R 3480
Reconstituted Complex Homo sapiens
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
44 ERBB3 2065
Protein-peptide Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
45 EHD2 30846
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 AVPR1B  
Affinity Capture-MS Homo sapiens
47 VSIG4  
Affinity Capture-MS Homo sapiens
48 ITGB3BP  
Two-hybrid Homo sapiens
49 SOCS6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 DNAJC5 80331
Proximity Label-MS Homo sapiens
51 PTP4A3  
Proximity Label-MS Homo sapiens
52 DRAP1 10589
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
53 PIK3CB 5291
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 IL20RA  
Affinity Capture-MS Homo sapiens
55 ALDH3B1 221
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 FLRT1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 LUC7L2 51631
Two-hybrid Homo sapiens
58 PIK3R1 5295
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
59 TRMT2A 27037
Two-hybrid Homo sapiens
60 PRKAR1B  
Two-hybrid Homo sapiens
61 LINC00238  
Two-hybrid Homo sapiens
62 DSN1 79980
Two-hybrid Homo sapiens
63 TMEM52B 120939
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
64 RING1 6015
Two-hybrid Homo sapiens
65 DISC1 27185
Two-hybrid Homo sapiens
66 RUSC1  
Two-hybrid Homo sapiens
67 GPC4 2239
Affinity Capture-MS Homo sapiens
68 C9orf24  
Two-hybrid Homo sapiens
69 LAMP1 3916
Proximity Label-MS Homo sapiens
70 ERBB4 2066
Reconstituted Complex Homo sapiens
71 EGFR 1956
Protein-peptide Homo sapiens
Two-hybrid Homo sapiens
Co-localization Homo sapiens
PCA Homo sapiens
Reconstituted Complex Homo sapiens
72 PTK2 5747
Two-hybrid Homo sapiens
73 KIAA1377  
Two-hybrid Homo sapiens
74 FSD2 123722
Two-hybrid Homo sapiens
75 RIPK4  
Affinity Capture-MS Homo sapiens
76 VBP1 7411
Two-hybrid Homo sapiens
77 BLK 640
Two-hybrid Homo sapiens
78 KCNE3  
Affinity Capture-MS Homo sapiens
79 L3MBTL3  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
80 RELT  
Affinity Capture-MS Homo sapiens
81 FAM208B 54906
Two-hybrid Homo sapiens
82 PIK3CA 5290
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
83 MET 4233
Reconstituted Complex Homo sapiens
84 AMBP 259
Two-hybrid Homo sapiens
85 OLFM3  
Affinity Capture-MS Homo sapiens
86 EFNB2 1948
Affinity Capture-MS Homo sapiens
87 IL6R  
Negative Genetic Homo sapiens
88 GRB2 2885
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
89 GAB1  
Reconstituted Complex Homo sapiens
90 ABCB6 10058
Two-hybrid Homo sapiens
91 TGFBR2 7048
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
92 LNX2  
Two-hybrid Homo sapiens
93 DOCK5 80005
Affinity Capture-MS Homo sapiens
94 C3orf36  
Two-hybrid Homo sapiens
95 NLGN3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
96 HAVCR2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
97 IRS2 8660
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
98 CCDC89  
Two-hybrid Homo sapiens
99 FGB 2244
Two-hybrid Homo sapiens
100 NISCH 11188
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
101 CHRM3 1131
Affinity Capture-MS Homo sapiens
102 SKAP1 8631
Affinity Capture-MS Homo sapiens
103 PPP4R1L  
Affinity Capture-MS Homo sapiens
104 ZNF620  
Two-hybrid Homo sapiens
105 NAB2 4665
Two-hybrid Homo sapiens
106 C16orf54 283897
Affinity Capture-MS Homo sapiens
107 EPHA2 1969
Proximity Label-MS Homo sapiens
108 PIK3R2 5296
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
109 AR 367
Reconstituted Complex Homo sapiens
110 FAM173A  
Two-hybrid Homo sapiens
111 BLZF1  
Two-hybrid Homo sapiens
112 IRS1 3667
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
113 KIAA0408  
Two-hybrid Homo sapiens
114 SERF1A  
Two-hybrid Homo sapiens
115 TNNI1 7135
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which PIK3R3 is involved
PathwayEvidenceSource
Adaptive Immune System TAS Reactome
Axon guidance TAS Reactome
CD28 co-stimulation TAS Reactome
CD28 dependent PI3K/Akt signaling TAS Reactome
Constitutive Signaling by Aberrant PI3K in Cancer TAS Reactome
Costimulation by the CD28 family TAS Reactome
Cytokine Signaling in Immune system TAS Reactome
Cytokine Signaling in Immune system IEA Reactome
Developmental Biology TAS Reactome
Disease TAS Reactome
Diseases of signal transduction by growth factor receptors and second messengers TAS Reactome
ESR-mediated signaling TAS Reactome
Extra-nuclear estrogen signaling TAS Reactome
G alpha (q) signalling events TAS Reactome
GPCR downstream signalling TAS Reactome
GPVI-mediated activation cascade TAS Reactome
Hemostasis TAS Reactome
Immune System TAS Reactome
Immune System IEA Reactome
Interleukin receptor SHC signaling TAS Reactome
Interleukin-2 family signaling TAS Reactome
Interleukin-3, Interleukin-5 and GM-CSF signaling TAS Reactome
Interleukin-3, Interleukin-5 and GM-CSF signaling IEA Reactome
Interleukin-7 signaling IEA Reactome
Interleukin-7 signaling TAS Reactome
Intracellular signaling by second messengers TAS Reactome
Metabolism TAS Reactome
Metabolism of lipids TAS Reactome
Negative regulation of the PI3K/AKT network TAS Reactome
Nervous system development TAS Reactome
Phospholipid metabolism TAS Reactome
PI Metabolism TAS Reactome
PI3K/AKT Signaling in Cancer TAS Reactome
PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling TAS Reactome
PIP3 activates AKT signaling TAS Reactome
Platelet activation, signaling and aggregation TAS Reactome
RAC1 GTPase cycle TAS Reactome
RAC2 GTPase cycle TAS Reactome
Regulation of signaling by CBL TAS Reactome
Regulation of signaling by CBL IEA Reactome
RET signaling TAS Reactome
RHO GTPase cycle TAS Reactome
Signal Transduction TAS Reactome
Signaling by GPCR TAS Reactome
Signaling by Interleukins TAS Reactome
Signaling by Interleukins IEA Reactome
Signaling by KIT in disease TAS Reactome
Signaling by Nuclear Receptors TAS Reactome
Signaling by phosphorylated juxtamembrane, extracellular and kinase domain KIT mutants TAS Reactome
Signaling by Receptor Tyrosine Kinases TAS Reactome
Signaling by Rho GTPases TAS Reactome
Signaling by Rho GTPases, Miro GTPases and RHOBTB3 TAS Reactome
Signaling by SCF-KIT TAS Reactome
Synthesis of PIPs at the plasma membrane TAS Reactome





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