Gene description for CUL3
Gene name cullin 3
Gene symbol CUL3
Other names/aliases CUL-3
PHA2E
Species Homo sapiens
 Database cross references - CUL3
ExoCarta ExoCarta_8452
Vesiclepedia VP_8452
Entrez Gene 8452
HGNC 2553
MIM 603136
UniProt Q13618  
 CUL3 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Colorectal cancer cells 34887515    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Hepatocellular carcinoma cells 26054723    
Mesenchymal stem cells 36408942    
Neuroblastoma cells 25944692    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Thymus 23844026    
Urine 19056867    
 Gene ontology annotations for CUL3
Molecular Function
    ubiquitin-protein transferase activity GO:0004842 IDA
    Notch binding GO:0005112 IPI
    protein binding GO:0005515 IPI
    cyclin binding GO:0030332 IEA
    POZ domain binding GO:0031208 IDA
    ubiquitin protein ligase binding GO:0031625 IBA
    ubiquitin protein ligase binding GO:0031625 IPI
    identical protein binding GO:0042802 IEA
    ubiquitin protein ligase activity GO:0061630 IDA
    ubiquitin ligase complex scaffold activity GO:0160072 IDA
Biological Process
    G1/S transition of mitotic cell cycle GO:0000082 TAS
    negative regulation of transcription by RNA polymerase II GO:0000122 IEA
    protein polyubiquitination GO:0000209 IDA
    kidney development GO:0001822 IEA
    trophectodermal cellular morphogenesis GO:0001831 IEA
    ubiquitin-dependent protein catabolic process GO:0006511 IDA
    ubiquitin-dependent protein catabolic process GO:0006511 IDA
    protein monoubiquitination GO:0006513 IDA
    endoplasmic reticulum to Golgi vesicle-mediated transport GO:0006888 IDA
    inflammatory response GO:0006954 IEA
    mitotic metaphase chromosome alignment GO:0007080 IMP
    integrin-mediated signaling pathway GO:0007229 ISS
    gastrulation GO:0007369 IEA
    positive regulation of cell population proliferation GO:0008284 TAS
    gene expression GO:0010467 IEA
    Wnt signaling pathway GO:0016055 IEA
    cell migration GO:0016477 IMP
    protein ubiquitination GO:0016567 IBA
    protein ubiquitination GO:0016567 IDA
    stem cell division GO:0017145 ISS
    cell projection organization GO:0030030 IEA
    anaphase-promoting complex-dependent catabolic process GO:0031145 IDA
    positive regulation of protein ubiquitination GO:0031398 IGI
    protein destabilization GO:0031648 IGI
    positive regulation of cytokinesis GO:0032467 IMP
    negative regulation of type I interferon production GO:0032480 IDA
    cellular response to oxidative stress GO:0034599 IDA
    negative regulation of Rho protein signal transduction GO:0035024 IMP
    embryonic cleavage GO:0040016 ISS
    stress fiber assembly GO:0043149 IMP
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 IDA
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 IMP
    fibroblast apoptotic process GO:0044346 IEA
    positive regulation of mitotic metaphase/anaphase transition GO:0045842 IMP
    COPII vesicle coating GO:0048208 IMP
    protein autoubiquitination GO:0051865 IDA
    protein K48-linked ubiquitination GO:0070936 IDA
    cellular response to amino acid stimulus GO:0071230 IDA
    nuclear protein quality control by the ubiquitin-proteasome system GO:0071630 IDA
    liver morphogenesis GO:0072576 IEA
    intrinsic apoptotic signaling pathway GO:0097193 TAS
    regulation protein catabolic process at postsynapse GO:0140252 IEA
    regulation of cellular response to insulin stimulus GO:1900076 IDA
    positive regulation of mitotic cell cycle phase transition GO:1901992 IMP
    positive regulation of TORC1 signaling GO:1904263 IDA
Subcellular Localization
    spindle pole GO:0000922 IDA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 TAS
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 IDA
    Golgi apparatus GO:0005794 IEA
    centrosome GO:0005813 IDA
    polar microtubule GO:0005827 IDA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IDA
    membrane GO:0016020 HDA
    Cul3-RING ubiquitin ligase complex GO:0031463 IBA
    Cul3-RING ubiquitin ligase complex GO:0031463 IDA
    sperm flagellum GO:0036126 IDA
    extracellular exosome GO:0070062 HDA
    mitotic spindle GO:0072686 IDA
    postsynapse GO:0098794 IEA
    glutamatergic synapse GO:0098978 IEA
 Experiment description of studies that identified CUL3 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 426
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 427
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
8
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
10
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
11
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
12
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
13
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
14
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
15
Experiment ID 235
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
16
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
17
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
18
Experiment ID 434
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
19
Experiment ID 435
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
20
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
21
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for CUL3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 EIF3A 8661
Affinity Capture-MS Homo sapiens
2 NPEPPS 9520
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 NOTCH3 4854
Affinity Capture-MS Homo sapiens
4 ZNF24  
Affinity Capture-MS Homo sapiens
5 ZFP1  
Affinity Capture-MS Homo sapiens
6 LRRC42  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 GMDS 2762
Affinity Capture-MS Homo sapiens
8 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
9 ZNF528  
Affinity Capture-MS Homo sapiens
10 CAP1 10487
Affinity Capture-MS Homo sapiens
11 CAND2 23066
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 STK39 27347
Affinity Capture-MS Homo sapiens
13 SLC2A1 6513
Affinity Capture-MS Homo sapiens
14 MYO10 4651
Affinity Capture-MS Homo sapiens
15 ZNF432  
Affinity Capture-MS Homo sapiens
16 UBC 7316
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Biochemical Activity Homo sapiens
17 VHL  
Affinity Capture-MS Homo sapiens
18 INTS1 26173
Affinity Capture-MS Homo sapiens
19 TAF15 8148
Affinity Capture-MS Homo sapiens
20 GCN1L1 10985
Affinity Capture-MS Homo sapiens
21 MLLT11  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 MASTL  
Affinity Capture-MS Homo sapiens
23 WDR76  
Affinity Capture-MS Homo sapiens
24 DTX3  
Affinity Capture-MS Homo sapiens
25 DARS 1615
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 MRPL13  
Affinity Capture-MS Homo sapiens
27 GFPT1 2673
Affinity Capture-MS Homo sapiens
28 ISYNA1 51477
Affinity Capture-MS Homo sapiens
29 CIAPIN1 57019
Affinity Capture-MS Homo sapiens
30 DRG1 4733
Affinity Capture-MS Homo sapiens
31 BRCC3 79184
Affinity Capture-MS Homo sapiens
32 ERC1 23085
Affinity Capture-MS Homo sapiens
33 ABCD3 5825
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 RPS9 6203
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 COPG1 22820
Affinity Capture-MS Homo sapiens
36 FAF1 11124
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
37 ZC3HAV1 56829
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 RIPK2  
Affinity Capture-MS Homo sapiens
39 NSFL1C 55968
Affinity Capture-Western Homo sapiens
40 PPP6R3 55291
Affinity Capture-MS Homo sapiens
41 ZMYM1 79830
Affinity Capture-MS Homo sapiens
42 TRIM4 89122
Affinity Capture-MS Homo sapiens
43 FAM120A 23196
Affinity Capture-MS Homo sapiens
44 KIF14 9928
Affinity Capture-MS Homo sapiens
45 EMC1 23065
Affinity Capture-MS Homo sapiens
46 AGO2 27161
Affinity Capture-MS Homo sapiens
47 UNK  
Affinity Capture-MS Homo sapiens
48 MPI 4351
Affinity Capture-MS Homo sapiens
49 AURKA 6790
Affinity Capture-Western Homo sapiens
50 SNAP47 116841
Affinity Capture-MS Homo sapiens
51 TCP1 6950
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 CPSF3L 54973
Affinity Capture-MS Homo sapiens
53 ARAF 369
Affinity Capture-MS Homo sapiens
54 SRPRB 58477
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
55 TRAFD1  
Affinity Capture-MS Homo sapiens
56 CTBP1 1487
Affinity Capture-MS Homo sapiens
57 CEP192 55125
Affinity Capture-MS Homo sapiens
58 MTFR1L 56181
Affinity Capture-MS Homo sapiens
59 RBM4B  
Affinity Capture-MS Homo sapiens
60 SIMC1  
Affinity Capture-MS Homo sapiens
61 TUFM 7284
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
62 PPIH 10465
Affinity Capture-MS Homo sapiens
63 EEF1E1 9521
Affinity Capture-MS Homo sapiens
64 NOC4L 79050
Affinity Capture-MS Homo sapiens
65 SYNCRIP 10492
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 FRAS1 80144
Affinity Capture-MS Homo sapiens
67 ENO1 2023
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
68 TUBA1C 84790
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
69 CCDC22 28952
Co-fractionation Homo sapiens
70 ANAPC4 29945
Affinity Capture-MS Homo sapiens
71 DNAJB2 3300
Affinity Capture-MS Homo sapiens
72 CUL2 8453
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
73 RRP1B 23076
Affinity Capture-MS Homo sapiens
74 CDX1  
Affinity Capture-MS Homo sapiens
75 MTMR4  
Affinity Capture-MS Homo sapiens
76 IQGAP2 10788
Affinity Capture-MS Homo sapiens
77 ZNF773  
Affinity Capture-MS Homo sapiens
78 SLC25A5 292
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
79 MAP2K7 5609
Affinity Capture-MS Homo sapiens
80 ADAM10 102
Affinity Capture-MS Homo sapiens
81 TRIP13 9319
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
82 HSPA5 3309
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
83 RBM39 9584
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
84 PPP2R2B 5521
Affinity Capture-MS Homo sapiens
85 HADHA 3030
Affinity Capture-MS Homo sapiens
86 NASP 4678
Affinity Capture-MS Homo sapiens
87 LRRC59 55379
Affinity Capture-MS Homo sapiens
88 LMNB1 4001
Affinity Capture-MS Homo sapiens
89 SRSF4 6429
Affinity Capture-MS Homo sapiens
90 PRKAR1A 5573
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
91 CD274 29126
Affinity Capture-Western Homo sapiens
92 NEBL 10529
Affinity Capture-MS Homo sapiens
93 BCDIN3D  
Affinity Capture-MS Homo sapiens
94 CSE1L 1434
Affinity Capture-MS Homo sapiens
95 ZNF250  
Affinity Capture-MS Homo sapiens
96 EPRS 2058
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
97 EIF2S2 8894
Affinity Capture-MS Homo sapiens
98 ZC3H11A