Gene description for TGFB1
Gene name transforming growth factor, beta 1
Gene symbol TGFB1
Other names/aliases CED
DPD1
LAP
TGFB
TGFbeta
Species Homo sapiens
 Database cross references - TGFB1
ExoCarta ExoCarta_7040
Vesiclepedia VP_7040
Entrez Gene 7040
HGNC 11766
MIM 190180
UniProt P01137  
 TGFB1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells Unpublished / Not applicable
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Prostate cancer cells 25857301    
Serum 19109410    
 Gene ontology annotations for TGFB1
Molecular Function
    type II transforming growth factor beta receptor binding GO:0005114 IDA
    type II transforming growth factor beta receptor binding GO:0005114 IMP
    type II transforming growth factor beta receptor binding GO:0005114 IPI
    cytokine activity GO:0005125 IBA
    cytokine activity GO:0005125 IDA
    cytokine activity GO:0005125 TAS
    protein binding GO:0005515 IPI
    growth factor activity GO:0008083 IMP
    enzyme binding GO:0019899 IPI
    type I transforming growth factor beta receptor binding GO:0034713 IMP
    type I transforming growth factor beta receptor binding GO:0034713 IPI
    type III transforming growth factor beta receptor binding GO:0034714 IMP
    type III transforming growth factor beta receptor binding GO:0034714 IPI
    deubiquitinase activator activity GO:0035800 IDA
    identical protein binding GO:0042802 IPI
    protein serine/threonine kinase activator activity GO:0043539 IDA
    protein-containing complex binding GO:0044877 IEA
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 IEA
    cell morphogenesis GO:0000902 IEA
    vasculogenesis GO:0001570 ISS
    ureteric bud development GO:0001657 IEA
    epithelial to mesenchymal transition GO:0001837 IDA
    epithelial to mesenchymal transition GO:0001837 IDA
    epithelial to mesenchymal transition GO:0001837 IDA
    epithelial to mesenchymal transition GO:0001837 TAS
    neural tube closure GO:0001843 ISS
    regulation of sodium ion transport GO:0002028 IEA
    sprouting angiogenesis GO:0002040 IMP
    chondrocyte differentiation GO:0002062 IDA
    columnar/cuboidal epithelial cell maturation GO:0002069 IEA
    hematopoietic progenitor cell differentiation GO:0002244 IDA
    connective tissue replacement involved in inflammatory response wound healing GO:0002248 TAS
    adaptive immune response based on somatic recombination of immune receptors built from immunoglobulin superfamily domains GO:0002460 IEA
    tolerance induction to self antigen GO:0002513 IEA
    negative regulation of natural killer cell mediated cytotoxicity directed against tumor cell target GO:0002859 IDA
    heart valve morphogenesis GO:0003179 ISS
    aortic valve morphogenesis GO:0003180 IMP
    protein export from nucleus GO:0006611 IDA
    ATP biosynthetic process GO:0006754 IDA
    phosphate-containing compound metabolic process GO:0006796 IDA
    intracellular calcium ion homeostasis GO:0006874 IEA
    apoptotic process GO:0006915 IMP
    transforming growth factor beta receptor signaling pathway GO:0007179 IBA
    transforming growth factor beta receptor signaling pathway GO:0007179 IDA
    transforming growth factor beta receptor signaling pathway GO:0007179 IGI
    transforming growth factor beta receptor signaling pathway GO:0007179 IMP
    transforming growth factor beta receptor signaling pathway GO:0007179 ISS
    Notch signaling pathway GO:0007219 IEA
    negative regulation of neuroblast proliferation GO:0007406 IEA
    salivary gland morphogenesis GO:0007435 IEP
    endoderm development GO:0007492 IEA
    heart development GO:0007507 ISS
    female pregnancy GO:0007565 IEA
    positive regulation of cell population proliferation GO:0008284 IDA
    positive regulation of cell population proliferation GO:0008284 IMP
    negative regulation of cell population proliferation GO:0008285 IDA
    negative regulation of cell population proliferation GO:0008285 IMP
    germ cell migration GO:0008354 IEA
    response to xenobiotic stimulus GO:0009410 IEA
    response to wounding GO:0009611 IEP
    gene expression GO:0010467 IEA
    positive regulation of vascular endothelial growth factor production GO:0010575 TAS
    positive regulation of gene expression GO:0010628 IDA
    positive regulation of gene expression GO:0010628 IGI
    positive regulation of gene expression GO:0010628 IMP
    positive regulation of gene expression GO:0010628 ISS
    positive regulation of gene expression GO:0010628 NAS
    negative regulation of gene expression GO:0010629 IDA
    negative regulation of gene expression GO:0010629 IDA
    negative regulation of gene expression GO:0010629 IGI
    negative regulation of gene expression GO:0010629 IGI
    negative regulation of gene expression GO:0010629 ISS
    negative regulation of gene expression GO:0010629 NAS
    negative regulation of extracellular matrix disassembly GO:0010716 TAS
    positive regulation of epithelial to mesenchymal transition GO:0010718 IDA
    positive regulation of epithelial to mesenchymal transition GO:0010718 IMP
    positive regulation of epithelial to mesenchymal transition GO:0010718 NAS
    macrophage derived foam cell differentiation GO:0010742 IC
    positive regulation of fibroblast migration GO:0010763 IDA
    negative regulation of macrophage cytokine production GO:0010936 IDA
    oligodendrocyte development GO:0014003 IEA
    positive regulation of microglia differentiation GO:0014008 ISS
    regulation of striated muscle tissue development GO:0016202 ISS
    neural tube development GO:0021915 ISS
    hyaluronan catabolic process GO:0030214 IDA
    negative regulation of ossification GO:0030279 IEA
    negative regulation of cell growth GO:0030308 IDA
    osteoclast differentiation GO:0030316 IEA
    regulation of cell migration GO:0030334 TAS
    positive regulation of cell migration GO:0030335 IDA
    positive regulation of cell migration GO:0030335 IMP
    membrane protein intracellular domain proteolysis GO:0031293 IDA
    positive regulation of protein-containing complex assembly GO:0031334 IDA
    positive regulation of exit from mitosis GO:0031536 IEA
    response to estradiol GO:0032355 IDA
    response to progesterone GO:0032570 IDA
    regulation of interleukin-23 production GO:0032667 IEA
    negative regulation of interleukin-17 production GO:0032700 IEA
    positive regulation of interleukin-17 production GO:0032740 IDA
    positive regulation of interleukin-6 production GO:0032755 IMP
    positive regulation of interleukin-6 production GO:0032755 IMP
    positive regulation of tumor necrosis factor production GO:0032760 IMP
    receptor catabolic process GO:0032801 IDA
    positive regulation of superoxide anion generation GO:0032930 IDA
    regulation of actin cytoskeleton organization GO:0032956 IEA
    positive regulation of collagen biosynthetic process GO:0032967 IDA
    positive regulation of collagen biosynthetic process GO:0032967 IGI
    positive regulation of collagen biosynthetic process GO:0032967 IMP
    response to vitamin D GO:0033280 IEA
    response to laminar fluid shear stress GO:0034616 IEA
    response to immobilization stress GO:0035902 IEA
    myofibroblast differentiation GO:0036446 NAS
    T cell proliferation GO:0042098 IEA
    regulation of cell population proliferation GO:0042127 IBA
    negative regulation of T cell proliferation GO:0042130 IEA
    regulation of protein import into nucleus GO:0042306 ISS
    positive regulation of protein import into nucleus GO:0042307 IDA
    odontogenesis of dentin-containing tooth GO:0042475 IEA
    positive regulation of odontogenesis GO:0042482 IEA
    myelination GO:0042552 IEA
    T cell homeostasis GO:0043029 IEA
    positive regulation of vascular permeability GO:0043117 IDA
    positive regulation of canonical NF-kappaB signal transduction GO:0043123 IMP
    surfactant homeostasis GO:0043129 IEA
    positive regulation of MAPK cascade GO:0043410 IDA
    positive regulation of blood vessel endothelial cell migration GO:0043536 IDA
    negative regulation of blood vessel endothelial cell migration GO:0043537 IDA
    regulatory T cell differentiation GO:0045066 IDA
    cell-cell junction organization GO:0045216 IDA
    positive regulation of regulatory T cell differentiation GO:0045591 IEA
    negative regulation of cell differentiation GO:0045596 IEP
    negative regulation of fat cell differentiation GO:0045599 IDA
    negative regulation of myoblast differentiation GO:0045662 IDA
    positive regulation of epidermal growth factor receptor signaling pathway GO:0045742 IDA
    negative regulation of cell cycle GO:0045786 IDA
    negative regulation of DNA-templated transcription GO:0045892 IDA
    positive regulation of DNA-templated transcription GO:0045893 IDA
    positive regulation of DNA-templated transcription GO:0045893 ISS
    positive regulation of transcription by RNA polymerase II GO:0045944 IDA
    muscle cell cellular homeostasis GO:0046716 IEA
    positive regulation of fibroblast proliferation GO:0048146 IEA
    lung alveolus development GO:0048286 IEA
    positive regulation of isotype switching to IgA isotypes GO:0048298 IDA
    lymph node development GO:0048535 ISS
    digestive tract development GO:0048565 IEA
    negative regulation of skeletal muscle tissue development GO:0048642 IDA
    positive regulation of smooth muscle cell proliferation GO:0048661 IDA
    inner ear development GO:0048839 IEA
    epithelial cell proliferation GO:0050673 IEA
    positive regulation of epithelial cell proliferation GO:0050679 IEA
    negative regulation of epithelial cell proliferation GO:0050680 IDA
    negative regulation of epithelial cell proliferation GO:0050680 IMP
    positive regulation of protein secretion GO:0050714 IDA
    positive regulation of inflammatory response GO:0050729 IMP
    positive regulation of peptidyl-tyrosine phosphorylation GO:0050731 IDA
    negative regulation of phagocytosis GO:0050765 IEA
    defense response to fungus GO:0050832 IEA
    positive regulation of chemotaxis GO:0050921 IDA
    positive regulation of smooth muscle cell differentiation GO:0051152 IEA
    positive regulation of protein metabolic process GO:0051247 IDA
    negative regulation of release of sequestered calcium ion into cytosol GO:0051280 IEA
    neuron apoptotic process GO:0051402 IEA
    positive regulation of cell division GO:0051781 IEA
    positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0051897 IDA
    ventricular cardiac muscle tissue morphogenesis GO:0055010 ISS
    phospholipid homeostasis GO:0055091 IEA
    canonical Wnt signaling pathway GO:0060070 IEA
    regulation of blood vessel remodeling GO:0060312 NAS
    regulation of blood vessel remodeling GO:0060312 TAS
    face morphogenesis GO:0060325 IEA
    frontal suture morphogenesis GO:0060364 IEA
    positive regulation of SMAD protein signal transduction GO:0060391 IDA
    positive regulation of SMAD protein signal transduction GO:0060391 IGI
    positive regulation of SMAD protein signal transduction GO:0060391 IMP
    bronchiole development GO:0060435 IEA
    mammary gland branching involved in thelarche GO:0060744 IEA
    branch elongation involved in mammary gland duct branching GO:0060751 IEA
    regulation of branching involved in mammary gland duct morphogenesis GO:0060762 IEA
    regulation of cartilage development GO:0061035 IEA
    retina vasculature development in camera-type eye GO:0061298 IEA
    Langerhans cell differentiation GO:0061520 IEA
    negative regulation of biomineral tissue development GO:0070168 IDA
    regulation of enamel mineralization GO:0070173 IEA
    lens fiber cell differentiation GO:0070306 IEA
    positive regulation of ERK1 and ERK2 cascade GO:0070374 IDA
    response to cholesterol GO:0070723 IDA
    cellular response to mechanical stimulus GO:0071260 IEA
    cellular response to glucose stimulus GO:0071333 IEA
    cellular response to low-density lipoprotein particle stimulus GO:0071404 IMP
    cellular response to hypoxia GO:0071456 IMP
    cellular response to ionizing radiation GO:0071479 IEA
    cellular response to dexamethasone stimulus GO:0071549 IEA
    cellular response to transforming growth factor beta stimulus GO:0071560 IDA
    cellular response to transforming growth factor beta stimulus GO:0071560 IGI
    positive regulation of mononuclear cell migration GO:0071677 IEA
    odontoblast differentiation GO:0071895 ISS
    stem cell proliferation GO:0072089 IEA
    extracellular matrix assembly GO:0085029 IDA
    positive regulation of branching involved in ureteric bud morphogenesis GO:0090190 IEA
    positive regulation of canonical Wnt signaling pathway GO:0090263 IDA
    positive regulation of canonical Wnt signaling pathway GO:0090263 IMP
    extrinsic apoptotic signaling pathway GO:0097191 IDA
    liver regeneration GO:0097421 IEA
    cellular response to virus GO:0098586 IEA
    negative regulation of hyaluronan biosynthetic process GO:1900126 IDA
    positive regulation of protein localization to nucleus GO:1900182 IDA
    positive regulation of extracellular matrix assembly GO:1901203 ISS
    positive regulation of extracellular matrix assembly GO:1901203 NAS
    positive regulation of extracellular matrix assembly GO:1901203 TAS
    response to salt GO:1902074 IEA
    positive regulation of mesenchymal stem cell proliferation GO:1902462 IGI
    regulation of miRNA transcription GO:1902893 IMP
    negative regulation of miRNA transcription GO:1902894 IMP
    negative regulation of miRNA transcription GO:1902894 NAS
    positive regulation of miRNA transcription GO:1902895 IDA
    negative regulation of protein localization to plasma membrane GO:1903077 IDA
    positive regulation of vasculature development GO:1904018 IDA
    positive regulation of receptor signaling pathway via STAT GO:1904894 IDA
    regulation of epithelial to mesenchymal transition involved in endocardial cushion formation GO:1905005 ISS
    cellular response to acetaldehyde GO:1905641 IEA
    cellular response to insulin-like growth factor stimulus GO:1990314 IEA
    embryonic liver development GO:1990402 ISS
    negative regulation of cell-cell adhesion mediated by cadherin GO:2000048 IDA
    positive regulation of chemokine (C-X-C motif) ligand 2 production GO:2000343 IMP
    positive regulation of endothelial cell apoptotic process GO:2000353 IMP
    positive regulation of primary miRNA processing GO:2000636 IDA
    positive regulation of cardiac muscle cell differentiation GO:2000727 IDA
Subcellular Localization
    extracellular region GO:0005576 TAS
    extracellular space GO:0005615 IBA
    extracellular space GO:0005615 IC
    extracellular space GO:0005615 IDA
    nucleus GO:0005634 IDA
    cytoplasm GO:0005737 IDA
    Golgi lumen GO:0005796 TAS
    plasma membrane GO:0005886 TAS
    microvillus GO:0005902 IDA
    cell surface GO:0009986 IMP
    axon GO:0030424 IEA
    extracellular matrix GO:0031012 ISS
    platelet alpha granule lumen GO:0031093 TAS
    neuronal cell body GO:0043025 IEA
    collagen-containing extracellular matrix GO:0062023 HDA
    blood microparticle GO:0072562 HDA
 Experiment description of studies that identified TGFB1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 126
MISEV standards
✘ Biophysical techniques
✔
GAPDH
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim"
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
6
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 250
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|TSG101|CD63|CD81
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25857301    
Organism Homo sapiens
Experiment description Large oncosomes contain distinct protein cargo and represent a separate functional class of tumor-derived extracellular vesicles.
Authors "Minciacchi VR, You S, Spinelli C, Morley S, Zandian M, Aspuria PJ, Cavallini L, Ciardiello C, Reis Sobreiro M, Morello M, Kharmate G, Jang SC, Kim DK, Hosseini-Beheshti E, Tomlinson Guns E, Gleave M, Gho YS, Mathivanan S, Yang W, Freeman MR, Di Vizio D"
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - expressing DIAPH3 hRNA
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
10
Experiment ID 250
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|TSG101|CD63|CD81
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25857301    
Organism Homo sapiens
Experiment description Large oncosomes contain distinct protein cargo and represent a separate functional class of tumor-derived extracellular vesicles.
Authors "Minciacchi VR, You S, Spinelli C, Morley S, Zandian M, Aspuria PJ, Cavallini L, Ciardiello C, Reis Sobreiro M, Morello M, Kharmate G, Jang SC, Kim DK, Hosseini-Beheshti E, Tomlinson Guns E, Gleave M, Gho YS, Mathivanan S, Yang W, Freeman MR, Di Vizio D"
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - expressing DIAPH3 hRNA
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
11
Experiment ID 29
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 19109410    
Organism Homo sapiens
Experiment description Proteomic and immunologic analyses of brain tumor exosomes.
Authors "Graner MW, Alzate O, Dechkovskaia AM, Keene JD, Sampson JH, Mitchell DA, Bigner DD"
Journal name FASEB
Publication year 2008
Sample Serum
Sample name Serum - Brain tumor
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
 Protein-protein interactions for TGFB1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RPL11 6135
Affinity Capture-MS Homo sapiens
2 MMP2 4313
Biochemical Activity Homo sapiens
Co-localization Homo sapiens
3 FCN1 2219
Co-purification Homo sapiens
4 P4HB 5034
Two-hybrid Homo sapiens
5 MCM7 4176
Affinity Capture-MS Homo sapiens
6 RPL9 6133
Affinity Capture-MS Homo sapiens
7 SMC3 9126
Affinity Capture-MS Homo sapiens
8 FMOD 2331
Reconstituted Complex Homo sapiens
9 RAD51  
Affinity Capture-MS Homo sapiens
10 MCM4 4173
Affinity Capture-MS Homo sapiens
11 RETNLB  
Affinity Capture-MS Homo sapiens
12 GART 2618
Affinity Capture-MS Homo sapiens
13 RPS3A 6189
Affinity Capture-MS Homo sapiens
14 FSTL4  
Affinity Capture-MS Homo sapiens
15 YWHAE 7531
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
16 RPL7 6129
Affinity Capture-MS Homo sapiens
17 FCHO1  
Two-hybrid Homo sapiens
18 EPS15 2060
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
19 RPL10 6134
Affinity Capture-MS Homo sapiens
20 ITGB8 3696
Affinity Capture-MS Homo sapiens
21 OIT3 170392
Affinity Capture-MS Homo sapiens
22 SLURP1  
Affinity Capture-MS Homo sapiens
23 PDHA1 5160
Affinity Capture-MS Homo sapiens
24 SOX2  
Affinity Capture-MS Homo sapiens
25 APP 351
Co-localization Homo sapiens
26 RPS3 6188
Affinity Capture-MS Homo sapiens
27 TGFBR1 7046
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
28 NUDCD2 134492
Affinity Capture-MS Homo sapiens
29 LAMC1 3915
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 RPS6 6194
Affinity Capture-MS Homo sapiens
31 RUVBL1 8607
Affinity Capture-MS Homo sapiens
32 NDUFS3 4722
Affinity Capture-MS Homo sapiens
33 RPL24 6152
Affinity Capture-MS Homo sapiens
34 GFPT1 2673
Affinity Capture-MS Homo sapiens
35 SLC35G2  
Affinity Capture-MS Homo sapiens
36 IMPDH2 3615
Affinity Capture-MS Homo sapiens
37 C4orf48  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 HSPH1 10808
Affinity Capture-MS Homo sapiens
39 OBSL1 23363
Affinity Capture-MS Homo sapiens
40 SRRM2 23524
Affinity Capture-MS Homo sapiens
41 PLIN3 10226
Affinity Capture-MS Homo sapiens
42 KRTAP1-3  
Two-hybrid Homo sapiens
43 CLNS1A 1207
Affinity Capture-MS Homo sapiens
44 EFEMP1 2202
Affinity Capture-MS Homo sapiens
45 KRTAP4-11  
Two-hybrid Homo sapiens
46 IGFL3  
Affinity Capture-MS Homo sapiens
47 PLA2G10  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 TUBA4A 7277
Affinity Capture-MS Homo sapiens
49 PZP 5858
Reconstituted Complex Homo sapiens
50 LPXN 9404
Two-hybrid Homo sapiens
51 HNF1B  
Affinity Capture-MS Homo sapiens
52 PPP6R3 55291
Affinity Capture-MS Homo sapiens
53 NMT1 4836
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 WDR5 11091
Affinity Capture-MS Homo sapiens
55 RPS11 6205
Affinity Capture-MS Homo sapiens
56 RHOG 391
Affinity Capture-MS Homo sapiens
57 EEF1A1 1915
Affinity Capture-MS Homo sapiens
58 LCE1A  
Two-hybrid Homo sapiens
59 SRPK1 6732
Affinity Capture-MS Homo sapiens
60 HSPA8 3312
Affinity Capture-MS Homo sapiens
61 SMC4 10051
Affinity Capture-MS Homo sapiens
62 COL2A1 1280
Reconstituted Complex Homo sapiens
63 CCT2 10576
Affinity Capture-MS Homo sapiens
64 VTN 7448
Reconstituted Complex Homo sapiens
65 ADAMTSL4  
Two-hybrid Homo sapiens
66 TMEM25  
Affinity Capture-MS Homo sapiens
67 LCE2C  
Two-hybrid Homo sapiens
68 RHOA 387
Affinity Capture-MS Homo sapiens
69 ATP5A1 498
Affinity Capture-MS Homo sapiens
70 MKLN1  
Affinity Capture-MS Homo sapiens
71 FHL3 2275
Affinity Capture-MS Homo sapiens
72 EMD 2010
Affinity Capture-MS Homo sapiens
73 CTBP1 1487
Affinity Capture-MS Homo sapiens
74 RPS10 6204
Affinity Capture-MS Homo sapiens
75 ALDH18A1 5832
Affinity Capture-MS Homo sapiens
76 CCDC33 80125
Two-hybrid Homo sapiens
77 DOT1L 84444
Affinity Capture-MS Homo sapiens
78 LCE3A  
Two-hybrid Homo sapiens
79 TUFM 7284
Affinity Capture-MS Homo sapiens
80 COPB1 1315
Affinity Capture-MS Homo sapiens
81 ACACA 31
Affinity Capture-MS Homo sapiens
82 ABCF2 10061
Affinity Capture-MS Homo sapiens
83 LCE3D  
Two-hybrid Homo sapiens
84 CDH1 999
Affinity Capture-MS Homo sapiens
85 FIBIN  
Affinity Capture-MS Homo sapiens
86 DDX5 1655
Affinity Capture-MS Homo sapiens
87 KRTAP10-8  
Two-hybrid Homo sapiens
88 C1orf54  
Affinity Capture-MS Homo sapiens
89 SMAD3 4088
Affinity Capture-MS Homo sapiens
90 SUMO2 6613
Affinity Capture-MS Homo sapiens
91 THBS1 7057
Co-purification Homo sapiens
92 MEOX2  
Two-hybrid Homo sapiens
93 YWHAQ 10971
Affinity Capture-MS Homo sapiens
94 NMT2 9397
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
95 RPN1 6184
Affinity Capture-MS Homo sapiens
96 GANAB 23193
Affinity Capture-MS Homo sapiens
97 DDX1 1653
Affinity Capture-MS Homo sapiens
98 PRKDC 5591
Affinity Capture-MS Homo sapiens
99 FBLN5 10516
Affinity Capture-MS Homo sapiens
100 HECTD1 25831
Affinity Capture-MS Homo sapiens
101 TGFBR3 7049
Affinity Capture-Western Homo sapiens
102 CAPN1 823
Affinity Capture-MS Homo sapiens
103 GMPS 8833
Affinity Capture-MS Homo sapiens
104 DDX39B 7919
Affinity Capture-MS Homo sapiens
105 LCE3B  
Two-hybrid Homo sapiens
106 RPS15A 6210
Affinity Capture-MS Homo sapiens
107 ACTG1 71
Affinity Capture-MS Homo sapiens
108 ATP6V1E1 529
Affinity Capture-MS Homo sapiens
109 DAXX  
Co-localization Homo sapiens
110 RMND5A 64795
Affinity Capture-MS Homo sapiens
111 SLC25A5 292
Affinity Capture-MS Homo sapiens
112 POU4F2  
Two-hybrid Homo sapiens
113 LY86  
Affinity Capture-MS Homo sapiens
114 LY6H  
Affinity Capture-MS Homo sapiens
115 TRIP13 9319
Affinity Capture-MS Homo sapiens
116 LAMC3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
117 HSPA5 3309
Affinity Capture-MS Homo sapiens
118 TUBB 203068
Affinity Capture-MS Homo sapiens
119 BGN 633
Reconstituted Complex Homo sapiens
120 DEFA1 1667
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
121 CDK9 1025
Affinity Capture-MS Homo sapiens
122 KRT18 3875
Affinity Capture-MS Homo sapiens
123 EIF6 3692
Affinity Capture-MS Homo sapiens
124 BAG2 9532
Affinity Capture-MS Homo sapiens
125 ACVRL1 94
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
126 CCT6A 908
Affinity Capture-MS Homo sapiens
127 GGH 8836
Affinity Capture-MS Homo sapiens
128 PON2 5445
Affinity Capture-MS Homo sapiens
129 KCMF1 56888
Affinity Capture-MS Homo sapiens
130 PDGFRA 5156
Affinity Capture-MS Homo sapiens
131 PRG2  
Affinity Capture-MS Homo sapiens
132 SF1 7536
Affinity Capture-MS Homo sapiens
133 RPS8 6202
Affinity Capture-MS Homo sapiens
134 LTBP3 4054
Affinity Capture-MS Homo sapiens
135 PDIA3 2923
Affinity Capture-MS Homo sapiens
136 LRRC47 57470
Affinity Capture-MS Homo sapiens
137 LYZL1  
Affinity Capture-MS Homo sapiens
138 FBXO6 26270
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
139 PNLIPRP2  
Affinity Capture-MS Homo sapiens
140 LRRC32 2615
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
141 IL5RA  
Affinity Capture-MS Homo sapiens
142 POLR2A 5430
Affinity Capture-MS Homo sapiens
143 SMU1 55234
Affinity Capture-MS Homo sapiens
144 LAMTOR5 10542
Two-hybrid Homo sapiens
145 DNAJA1 3301
Affinity Capture-MS Homo sapiens
146 DCN 1634
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
147 EPHA7 2045
Affinity Capture-MS Homo sapiens
148 RPS27 6232
Affinity Capture-MS Homo sapiens
149 HOXA1 3198
Two-hybrid Homo sapiens
150 MAP2K3 5606
Affinity Capture-MS Homo sapiens
151 UBR1 197131
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
152 STAT1 6772
Affinity Capture-MS Homo sapiens
153 FNTA 2339
Two-hybrid Homo sapiens
154 PPP2R1A 5518
Affinity Capture-MS Homo sapiens
155 FXR2 9513
Affinity Capture-MS Homo sapiens
156 RPS13 6207
Affinity Capture-MS Homo sapiens
157 FBXO2 26232
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
158 RANBP9 10048
Affinity Capture-MS Homo sapiens
159 TIMM8B 26521
Affinity Capture-MS Homo sapiens
160 SCGB2A2  
Affinity Capture-MS Homo sapiens
161 DHPS 1725
Affinity Capture-MS Homo sapiens
162 CATSPER1  
Two-hybrid Homo sapiens
163 RPL12 6136
Affinity Capture-MS Homo sapiens
164 HSPA1A 3303
Affinity Capture-MS Homo sapiens
165 PSMC5 5705
Affinity Capture-MS Homo sapiens
166 EEF1B2 1933
Affinity Capture-MS Homo sapiens
167 DDB1 1642
Affinity Capture-MS Homo sapiens
168 LTBP1 4052
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
169 ADSS 159
Affinity Capture-MS Homo sapiens
170 RPS18 6222
Affinity Capture-MS Homo sapiens
171 SMC1A 8243
Affinity Capture-MS Homo sapiens
172 HSPB1 3315
Affinity Capture-MS Homo sapiens
173 HSPD1 3329
Affinity Capture-MS Homo sapiens
174 MMP9 4318
Biochemical Activity Homo sapiens
Co-localization Homo sapiens
175 HSPA9 3313
Affinity Capture-MS Homo sapiens
176 HLA-A 3105
Affinity Capture-MS Homo sapiens
177 CANX 821
Affinity Capture-MS Homo sapiens
178 CBWD1 55871
Affinity Capture-MS Homo sapiens
179 LCE1D  
Two-hybrid Homo sapiens
180 FHL2 2274
Affinity Capture-MS Homo sapiens
181 FTSJ3 117246
Affinity Capture-MS Homo sapiens
182 HUWE1 10075
Affinity Capture-MS Homo sapiens
183 LCE1C  
Two-hybrid Homo sapiens
184 PSMC3 5702
Affinity Capture-MS Homo sapiens
185 STUB1 10273
Affinity Capture-MS Homo sapiens
186 IMMT 10989
Affinity Capture-MS Homo sapiens
187 TGFB2 7042
Affinity Capture-MS Homo sapiens
188 RPL13 6137
Affinity Capture-MS Homo sapiens
189 LAMB1 3912
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
190 NRROS  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
191 UBR2 23304
Affinity Capture-MS Homo sapiens
192 CCT8 10694
Affinity Capture-MS Homo sapiens
193 CYSRT1 375791
Two-hybrid Homo sapiens
194 TGFBR2 7048
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
195 WDR77 79084
Affinity Capture-MS Homo sapiens
196 NSF 4905
Affinity Capture-MS Homo sapiens
197 SRRM1 10250
Affinity Capture-MS Homo sapiens
198 ERP44 23071
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
199 ATP1A3 478
Affinity Capture-MS Homo sapiens
200 RUVBL2 10856
Affinity Capture-MS Homo sapiens
201 EIF1AX 1964
Affinity Capture-MS Homo sapiens
202 DNAJA2 10294
Affinity Capture-MS Homo sapiens
203 DDX3X 1654
Affinity Capture-MS Homo sapiens
204 ADSL 158
Affinity Capture-MS Homo sapiens
205 SLC39A7 7922
Affinity Capture-MS Homo sapiens
206 ENG 2022
Affinity Capture-Western Homo sapiens
207 RPS12 6206
Affinity Capture-MS Homo sapiens
208 RPS4X 6191
Affinity Capture-MS Homo sapiens
209 SQSTM1 8878
Affinity Capture-MS Homo sapiens
210 THRAP3 9967
Affinity Capture-MS Homo sapiens
211 CDH23 64072
Affinity Capture-MS Homo sapiens
212 KRTAP1-1  
Two-hybrid Homo sapiens
213 ITPR3 3710
Affinity Capture-MS Homo sapiens
214 EIF4A3 9775
Affinity Capture-MS Homo sapiens
215 FKBP1A 2280
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
216 FEM1B 10116
Affinity Capture-MS Homo sapiens
217 RPS5 6193
Affinity Capture-MS Homo sapiens
218 NUP98 4928
Affinity Capture-MS Homo sapiens
219 HNRNPK 3190
Affinity Capture-MS Homo sapiens
220 STOML2 30968
Affinity Capture-MS Homo sapiens
221 PSMA1 5682
Affinity Capture-MS Homo sapiens
222 EIF4H 7458
Affinity Capture-MS Homo sapiens
223 PCNA 5111
Affinity Capture-MS Homo sapiens
224 EEF2 1938
Affinity Capture-MS Homo sapiens
225 LCE2B  
Two-hybrid Homo sapiens
226 AKAP8L  
Affinity Capture-MS Homo sapiens
227 TXN 7295
Affinity Capture-MS Homo sapiens
228 NUP107 57122
Affinity Capture-MS Homo sapiens
229 RPL38 6169
Affinity Capture-MS Homo sapiens
230 RPL27A 6157
Affinity Capture-MS Homo sapiens
231 PCBP1 5093
Affinity Capture-MS Homo sapiens
232 CAD 790
Affinity Capture-MS Homo sapiens
233 FLNA 2316
Affinity Capture-MS Homo sapiens
234 EIF5B 9669
Affinity Capture-MS Homo sapiens
235 IMPDH1 3614
Affinity Capture-MS Homo sapiens
236 EPHA2 1969
Affinity Capture-MS Homo sapiens
237 RPL23A 6147
Affinity Capture-MS Homo sapiens
238 EDN3  
Affinity Capture-MS Homo sapiens
239 HNRNPU 3192
Affinity Capture-MS Homo sapiens
240 GNB2L1 10399
Affinity Capture-MS Homo sapiens
241 HTR1B  
Affinity Capture-MS Homo sapiens
242 VASN 114990
Reconstituted Complex Homo sapiens
243 PTCH1  
Affinity Capture-MS Homo sapiens
244 MAGI1  
Affinity Capture-MS Homo sapiens
245 KIF4A 24137
Affinity Capture-MS Homo sapiens
246 CDC37 11140
Affinity Capture-MS Homo sapiens
247 RANGAP1 5905
Affinity Capture-MS Homo sapiens
248 EPRS 2058
Affinity Capture-MS Homo sapiens
249 CTNND1 1500
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which TGFB1 is involved
PathwayEvidenceSource
Adipogenesis IEA Reactome
Cell surface interactions at the vascular wall TAS Reactome
Cytokine Signaling in Immune system TAS Reactome
Deubiquitination IEA Reactome
Developmental Biology IEA Reactome
Disease TAS Reactome
Diseases of signal transduction by growth factor receptors and second messengers TAS Reactome
Downregulation of TGF-beta receptor signaling IEA Reactome
Downregulation of TGF-beta receptor signaling TAS Reactome
ECM proteoglycans IEA Reactome
Elastic fibre formation TAS Reactome
Extracellular matrix organization TAS Reactome
Extracellular matrix organization IEA Reactome
Gene expression (Transcription) TAS Reactome
Generic Transcription Pathway TAS Reactome
Hemostasis TAS Reactome
Immune System TAS Reactome
Infectious disease TAS Reactome
Influenza Infection TAS Reactome
Influenza Virus Induced Apoptosis TAS Reactome
Interleukin-4 and Interleukin-13 signaling TAS Reactome
Loss of Function of SMAD2/3 in Cancer TAS Reactome
Loss of Function of TGFBR1 in Cancer TAS Reactome
Loss of Function of TGFBR2 in Cancer TAS Reactome
Metabolism of proteins IEA Reactome
Molecules associated with elastic fibres TAS Reactome
Non-integrin membrane-ECM interactions TAS Reactome
Platelet activation, signaling and aggregation TAS Reactome
Platelet degranulation TAS Reactome
Post-translational protein modification IEA Reactome
Regulation of RUNX3 expression and activity TAS Reactome
Response to elevated platelet cytosolic Ca2+ TAS Reactome
RNA Polymerase II Transcription TAS Reactome
RUNX3 regulates CDKN1A transcription TAS Reactome
RUNX3 regulates p14-ARF TAS Reactome
Signal Transduction TAS Reactome
Signal Transduction IEA Reactome
Signaling by Interleukins TAS Reactome
Signaling by TGF-beta Receptor Complex TAS Reactome
Signaling by TGF-beta Receptor Complex IEA Reactome
Signaling by TGF-beta Receptor Complex in Cancer TAS Reactome
Signaling by TGFB family members TAS Reactome
Signaling by TGFB family members IEA Reactome
Signaling by TGFBR3 TAS Reactome
SMAD2/3 Phosphorylation Motif Mutants in Cancer TAS Reactome
Syndecan interactions TAS Reactome
TGF-beta receptor signaling activates SMADs TAS Reactome
TGF-beta receptor signaling activates SMADs IEA Reactome
TGF-beta receptor signaling in EMT (epithelial to mesenchymal transition) TAS Reactome
TGF-beta receptor signaling in EMT (epithelial to mesenchymal transition) IEA Reactome
TGFBR1 KD Mutants in Cancer TAS Reactome
TGFBR1 LBD Mutants in Cancer TAS Reactome
TGFBR2 Kinase Domain Mutants in Cancer TAS Reactome
TGFBR2 MSI Frameshift Mutants in Cancer TAS Reactome
TGFBR3 regulates TGF-beta signaling TAS Reactome
Transcriptional regulation by RUNX3 TAS Reactome
Transcriptional regulation of white adipocyte differentiation IEA Reactome
UCH proteinases IEA Reactome
Viral Infection Pathways TAS Reactome





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