Gene description for EFTUD1
Gene name elongation factor Tu GTP binding domain containing 1
Gene symbol EFTUD1
Other names/aliases FAM42A
HsT19294
RIA1
Species Homo sapiens
 Database cross references - EFTUD1
ExoCarta ExoCarta_79631
Vesiclepedia VP_79631
Entrez Gene 79631
HGNC 25789
UniProt Q7Z2Z2  
 EFTUD1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Chondrocytes 35931686    
Chondrocytes 35931686    
Mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for EFTUD1
Molecular Function
    translation elongation factor activity GO:0003746 IEA
    GTPase activity GO:0003924 IBA
    GTPase activity GO:0003924 IDA
    protein binding GO:0005515 IPI
    GTP binding GO:0005525 IEA
    ribosome binding GO:0043022 IBA
    ribosome binding GO:0043022 IMP
Biological Process
    translational elongation GO:0006414 IEA
    cytosolic ribosome assembly GO:0042256 IBA
    cytosolic ribosome assembly GO:0042256 IMP
    GTP metabolic process GO:0046039 IDA
Subcellular Localization
    cytosol GO:0005829 IBA
    ribonucleoprotein complex GO:1990904 IBA
 Experiment description of studies that identified EFTUD1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
8
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for EFTUD1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SASS6 163786
Affinity Capture-MS Homo sapiens
2 Haus1  
Affinity Capture-MS Mus musculus
3 KRT18 3875
Co-fractionation Homo sapiens
4 CDC14A  
Proximity Label-MS Homo sapiens
5 TRIP6 7205
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 ALAD 210
Co-fractionation Homo sapiens
7 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
8 FASN 2194
Positive Genetic Homo sapiens
9 FKBP10 60681
Co-fractionation Homo sapiens
10 TIPRL 261726
Co-fractionation Homo sapiens
11 EIF6 3692
Proximity Label-MS Homo sapiens
12 TPD52L2 7165
Co-fractionation Homo sapiens
13 LINC00839  
Protein-RNA Homo sapiens
14 FAM196A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 SSB 6741
Co-fractionation Homo sapiens
16 Rbm8a  
Affinity Capture-MS Mus musculus
17 Chmp4b 75608
Affinity Capture-MS Mus musculus
18 TCP1 6950
Co-fractionation Homo sapiens
19 PANK4 55229
Co-fractionation Homo sapiens
20 PLCG1 5335
Co-fractionation Homo sapiens
21 ROCK2 9475
Co-fractionation Homo sapiens
22 OSGEP 55644
Co-fractionation Homo sapiens
23 MID1 4281
Co-fractionation Homo sapiens
24 NSUN2 54888
Co-fractionation Homo sapiens
25 SBDS 51119
Co-fractionation Homo sapiens
26 TUBGCP4 27229
Affinity Capture-MS Homo sapiens
27 HEXA 3073
Co-fractionation Homo sapiens
28 Espl1  
Affinity Capture-MS Mus musculus
29 RIT1 6016
Negative Genetic Homo sapiens
30 EFTUD2 9343
Affinity Capture-MS Homo sapiens
31 MYCN  
Affinity Capture-MS Homo sapiens
32 ACTR2 10097
Co-fractionation Homo sapiens
33 KIF1BP 26128
Co-fractionation Homo sapiens
34 BAG1 573
Co-fractionation Homo sapiens
35 ABCF2 10061
Co-fractionation Homo sapiens
36 NTRK1 4914
Affinity Capture-MS Homo sapiens
37 PCGF6  
Affinity Capture-MS Homo sapiens
38 PTP4A2 8073
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 RPRD1B 58490
Co-fractionation Homo sapiens
40 NDC80 10403
Co-fractionation Homo sapiens
41 TP53RK 112858
Co-fractionation Homo sapiens
42 SKIV2L2 23517
Co-fractionation Homo sapiens
43 UQCRC1 7384
Co-fractionation Homo sapiens
44 Sptlc2  
Affinity Capture-MS Mus musculus
45 NFIC 4782
Co-fractionation Homo sapiens
46 Nedd1  
Affinity Capture-MS Mus musculus
47 FBL 2091
Proximity Label-MS Homo sapiens
48 HSPD1 3329
Cross-Linking-MS (XL-MS) Homo sapiens
49 AKT1 207
Co-fractionation Homo sapiens
50 LMNA 4000
Co-fractionation Homo sapiens
51 LPP 4026
Co-fractionation Homo sapiens
52 S100A2 6273
Affinity Capture-MS Homo sapiens
53 RANGAP1 5905
Co-fractionation Homo sapiens
54 DDX51  
Co-fractionation Homo sapiens
55 UFM1 51569
Co-fractionation Homo sapiens
56 Myl12a  
Affinity Capture-MS Mus musculus
View the network image/svg+xml
 Pathways in which EFTUD1 is involved
No pathways found





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here