Gene description for CD4
Gene name CD4 molecule
Gene symbol CD4
Other names/aliases CD4mut
Species Homo sapiens
 Database cross references - CD4
ExoCarta ExoCarta_920
Entrez Gene 920
HGNC 1678
MIM 186940
UniProt P01730  
 CD4 identified in exosomes derived from the following tissue/cell type
Thymus 23844026    
 Gene ontology annotations for CD4
Molecular Function
    protein homodimerization activity GO:0042803 IDA
    enzyme binding GO:0019899 IPI
    transmembrane signaling receptor activity GO:0004888 TAS
    protein binding GO:0005515 IPI
    protein kinase binding GO:0019901 IPI
    coreceptor activity GO:0015026 NAS
    extracellular matrix structural constituent GO:0005201 NAS
    zinc ion binding GO:0008270 IDA
    receptor activity GO:0004872 TAS
    glycoprotein binding GO:0001948 IPI
    MHC class II protein binding GO:0042289 NAS
Biological Process
    positive regulation of protein kinase activity GO:0045860 IDA
    entry into host cell GO:0030260 TAS
    induction by virus of host cell-cell fusion GO:0006948 IDA
    positive regulation of peptidyl-tyrosine phosphorylation GO:0050731 IEA
    immune response GO:0006955 NAS
    regulation of T cell activation GO:0050863 IDA
    viral process GO:0016032 TAS
    signal transduction GO:0007165 TAS
    transmembrane receptor protein tyrosine kinase signaling pathway GO:0007169 NAS
    enzyme linked receptor protein signaling pathway GO:0007167 TAS
    protein palmitoleylation GO:0045234 IDA
    T cell receptor signaling pathway GO:0050852 TAS
    cytokine production GO:0001816 IEA
    T cell differentiation GO:0030217 IDA
    positive regulation of interleukin-2 biosynthetic process GO:0045086 NAS
    regulation of defense response to virus by virus GO:0050690 TAS
    cell surface receptor signaling pathway GO:0007166 TAS
    defense response to Gram-negative bacterium GO:0050829 IEA
    T cell selection GO:0045058 IDA
    positive regulation of calcium-mediated signaling GO:0050850 IEA
    T cell costimulation GO:0031295 TAS
    maintenance of protein location in cell GO:0032507 IDA
    innate immune response GO:0045087 TAS
Subcellular Localization
    integral component of membrane GO:0016021 IEA
    external side of plasma membrane GO:0009897 IDA
    T cell receptor complex GO:0042101 NAS
    membrane raft GO:0045121 IEA
    plasma membrane GO:0005886 TAS
    early endosome GO:0005769 TAS
    endoplasmic reticulum lumen GO:0005788 IEA
    endoplasmic reticulum membrane GO:0005789 TAS
 Experiment description of studies that identified CD4 in exosomes
1
Experiment ID 217
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
CD81|CD9|CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for CD4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CD28  
Invitro Homo sapiens
2 LAT 27040
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
3 BTRC 8945
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
4 HLA-DPA1  
Affinity Capture-MS Homo sapiens
5 CXCR4 7852
Affinity Capture-Western Homo sapiens
6 TFRC 7037
Affinity Capture-MS Homo sapiens
7 PTPRC 5788
FRET Homo sapiens
8 PIP 5304
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
9 CD53 963
Invitro Homo sapiens
10 CD4 920
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 SPG21 51324
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
12 LCK 3932
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
13 LGALS1 3956
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
14 IL16  
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
15 UNC119  
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
16 PTK2 5747
Affinity Capture-MS Homo sapiens
17 DPP4 1803
Invitro Homo sapiens
18 CTSD 1509
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
19 FCGR3A  
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
20 CD5 921
Invitro Homo sapiens
21 IL2RA  
Invitro Homo sapiens
22 SLC3A2 6520
Affinity Capture-MS Homo sapiens
23 CCR5 1234
Affinity Capture-Western Homo sapiens
24 HLA-DQB1  
Affinity Capture-MS Homo sapiens
25 CD2 914
Affinity Capture-MS Homo sapiens
Invitro Homo sapiens
26 CD44 960
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
27 CD38 952
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
28 SELL 6402
Invitro Homo sapiens
29 CD82 3732
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
30 TNFRSF10A 8797
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here