Gene description for CNBP
Gene name CCHC-type zinc finger, nucleic acid binding protein
Gene symbol CNBP
Other names/aliases CNBP1
DM2
PROMM
RNF163
ZCCHC22
ZNF9
Species Homo sapiens
 Database cross references - CNBP
ExoCarta ExoCarta_7555
Vesiclepedia VP_7555
Entrez Gene 7555
HGNC 13164
MIM 116955
UniProt P62633  
 CNBP identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Foreskin fibroblasts 34108659    
Lymphoma cells 34108659    
Mammary cancer-associated fibroblasts 34108659    
Monocytic leukemia cells 34108659    
Normal mammary epithelial cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic duct epithalial cells 34108659    
Pancreatic duct epithalial cells 34108659    
Pluripotent stem cells 34108659    
T lymphocytes 34108659    
 Gene ontology annotations for CNBP
Molecular Function
    RNA binding GO:0003723 HDA
    single-stranded RNA binding GO:0003727 IBA
    mRNA binding GO:0003729 IBA
    protein binding GO:0005515 IPI
    zinc ion binding GO:0008270 IEA
    translation regulator activity GO:0045182 IBA
    G-quadruplex DNA binding GO:0051880 IDA
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 IDA
    regulation of DNA-templated transcription GO:0006355 TAS
    positive regulation of cell population proliferation GO:0008284 ISS
    cholesterol homeostasis GO:0042632 TAS
    positive regulation of DNA-templated transcription GO:0045893 ISS
    positive regulation of transcription by RNA polymerase II GO:0045944 ISS
    G-quadruplex DNA formation GO:0071919 IDA
    positive regulation of cytoplasmic translation GO:2000767 IBA
Subcellular Localization
    nucleus GO:0005634 ISS
    cytoplasm GO:0005737 IBA
    endoplasmic reticulum GO:0005783 ISS
    cytosol GO:0005829 ISS
    cytosol GO:0005829 TAS
 Experiment description of studies that identified CNBP in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 412
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX|ACTB
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MCF7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
6
Experiment ID 414
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
7
Experiment ID 426
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 427
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 407
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|CD81|SDCBP|TSG101|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
10
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
11
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
12
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
13
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
14
Experiment ID 405
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|CD81|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Foreskin fibroblasts
Sample name BJ
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
15
Experiment ID 417
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX|ACTB
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Lymphoma cells
Sample name Raji
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
16
Experiment ID 411
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Mammary cancer-associated fibroblasts
Sample name mCAF
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
17
Experiment ID 418
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Monocytic leukemia cells
Sample name THP-1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
18
Experiment ID 418
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Monocytic leukemia cells
Sample name THP-1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
19
Experiment ID 413
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Normal mammary epithelial cells
Sample name MCF10A
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
20
Experiment ID 406
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35|CD81
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name BxPC3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
21
Experiment ID 415
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
22
Experiment ID 434
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
23
Experiment ID 435
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
24
Experiment ID 408
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|CD81|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX|ACTB
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic duct epithalial cells
Sample name HPDE
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
25
Experiment ID 409
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic duct epithalial cells
Sample name HPNE
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
26
Experiment ID 416
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pluripotent stem cells
Sample name PSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
27
Experiment ID 410
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample T lymphocytes
Sample name Jurkat
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
 Protein-protein interactions for CNBP
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GNL2 29889
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 UBE2H 7328
Affinity Capture-MS Homo sapiens
3 IGF2BP1 10642
Affinity Capture-MS Homo sapiens
4 RPL26L1 51121
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 ZNF771  
Affinity Capture-MS Homo sapiens
6 RPS3A 6189
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 NOL12 79159
Affinity Capture-MS Homo sapiens
8 KLF9 687
Affinity Capture-MS Homo sapiens
9 RSL24D1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 C7orf50 84310
Affinity Capture-MS Homo sapiens
11 BZW2 28969
Co-fractionation Homo sapiens
12 BTF3 689
Affinity Capture-MS Homo sapiens
13 RPL13A 23521
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 RPL37A 6168
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 PAICS 10606
Affinity Capture-MS Homo sapiens
16 NOP56 10528
Affinity Capture-MS Homo sapiens
17 RPS19 6223
Affinity Capture-MS Homo sapiens
18 RPL15 6138
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 HNRNPC 3183
Affinity Capture-MS Homo sapiens
20 RPL14 9045
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 HNRNPR 10236
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
22 CADM1 23705
Co-fractionation Homo sapiens
23 USP36  
Affinity Capture-MS Homo sapiens
24 KAT7  
Affinity Capture-MS Homo sapiens
25 CENPU  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 RPL10A 4736
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 IGF2BP3 10643
Affinity Capture-MS Homo sapiens
28 MEAF6  
Affinity Capture-MS Homo sapiens
29 RBM28 55131
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 RPL7A 6130
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 DYRK1A 1859
Affinity Capture-MS Homo sapiens
32 KIF23 9493
Affinity Capture-MS Homo sapiens
33 DDX24 57062
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 CCDC137  
Affinity Capture-MS Homo sapiens
35 KLF6  
Affinity Capture-MS Homo sapiens
36 TRIM56 81844
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 MAPK14 1432
Affinity Capture-MS Homo sapiens
38 RPS9 6203
Affinity Capture-MS Homo sapiens
39 RPLP2 6181
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 RPF2 84154
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 TTF1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 PAK1IP1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 ITGA4 3676
Affinity Capture-MS Homo sapiens
44 FYTTD1  
Affinity Capture-MS Homo sapiens
45 ABT1 29777
Affinity Capture-MS Homo sapiens
46 RPS6 6194
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 VCAM1 7412
Affinity Capture-MS Homo sapiens
48 ZWINT  
Affinity Capture-MS Homo sapiens
49 KDM4D  
Affinity Capture-MS Homo sapiens
50 MYCN  
Affinity Capture-MS Homo sapiens
51 PLS3 5358
Co-fractionation Homo sapiens
52 PABPC4 8761
Co-fractionation Homo sapiens
53 ZNF184  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 RRP8  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
55 CCDC86  
Affinity Capture-MS Homo sapiens
56 FCF1  
Affinity Capture-MS Homo sapiens
57 Arhgap1 228359
Affinity Capture-MS Mus musculus
58 RBMX 27316
Affinity Capture-MS Homo sapiens
59 BRIX1 55299
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
60 AURKA 6790
Affinity Capture-MS Homo sapiens
61 RPL27 6155
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
62 DDX31  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
63 KIAA0020 9933
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
64 ING4  
Affinity Capture-MS Homo sapiens
65 DDX21 9188
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 KAT6B  
Affinity Capture-MS Homo sapiens
67 ZFP62  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
68 RPL4 6124
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
69 WHSC1 7468
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
70 HIST1H2AG 8969
Affinity Capture-MS Homo sapiens
71 MSX2  
Affinity Capture-MS Homo sapiens
72 STAU1 6780
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
73 IDH2 3418
Affinity Capture-MS Homo sapiens
74 APOBEC3C 27350
Affinity Capture-MS Homo sapiens
75 ITGB3BP  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
76 NOP2 4839
Affinity Capture-MS Homo sapiens
77 HIST1H3E 8353
Affinity Capture-MS Homo sapiens
78 ZNF22  
Affinity Capture-MS Homo sapiens
79 RPL5 6125
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
80 RPL18A 6142
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
81 VCP 7415
Affinity Capture-MS Homo sapiens
82 NKRF 55922
Affinity Capture-MS Homo sapiens
83 RPL31 6160
Affinity Capture-MS Homo sapiens
84 ARNT 405
Affinity Capture-MS Homo sapiens
85 SYNCRIP 10492
Affinity Capture-MS Homo sapiens
86 RPL36 25873
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
87 HIST1H1C 3006
Co-fractionation Homo sapiens
88 KLF10  
Affinity Capture-MS Homo sapiens
89 RPLP1 6176
Affinity Capture-MS Homo sapiens
90 DHX9 1660
Affinity Capture-MS Homo sapiens
91 RPLP0 6175
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
92 SARNP 84324
Affinity Capture-MS Homo sapiens
93 HNRNPA3 220988
Affinity Capture-MS Homo sapiens
94 DDX27 55661
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
95 GTPBP4 23560
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
96 GLTSCR2  
Affinity Capture-MS Homo sapiens
97 MYH4  
Affinity Capture-RNA Homo sapiens
98 CARS 833
Co-fractionation Homo sapiens
99 RPL30 6156
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
100 RAN 5901
Affinity Capture-MS Homo sapiens
101 HRH1  
Two-hybrid Homo sapiens
102 RSL1D1 26156
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
103 CENPN  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
104 PPAN-P2RY11  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
105 RPL32 6161
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
106 AHCY 191
Co-fractionation Homo sapiens
107 SMCHD1 23347
Affinity Capture-MS Homo sapiens
108 Cnbp 12785
Affinity Capture-MS Mus musculus
109 KCND3 3752
Affinity Capture-Western Homo sapiens
110 DAP3 7818
Affinity Capture-MS Homo sapiens
111 RPL13 6137
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
112 POP1 10940
Affinity Capture-MS Homo sapiens
113 EIF6 3692
Co-fractionation Homo sapiens
114 FERMT2 10979
Co-fractionation Homo sapiens
115 CUL3 8452
Affinity Capture-MS Homo sapiens
116 NR3C1 2908
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
117 RPL3 6122
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
118 EBNA1BP2 10969
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
119 PAPD5 64282
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
120 DHX30 22907
Affinity Capture-MS Homo sapiens
121 EPRS 2058
Co-fractionation Homo sapiens
122 HNRNPUL1 11100
Affinity Capture-MS Homo sapiens
123 RPL7 6129
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
124 SUZ12  
Affinity Capture-MS Homo sapiens
125 WEE1 7465
Affinity Capture-MS Homo sapiens
126 ZNF275  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
127 ERP44 23071
Affinity Capture-MS Homo sapiens
128 ZNF512  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
129 CDK2 1017
Affinity Capture-MS Homo sapiens
130 HIST1H1B 3009
Affinity Capture-MS Homo sapiens
131 HIST1H1A 3024
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
132 DKC1 1736
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
133 MYC  
Affinity Capture-MS Homo sapiens
134 RPA3 6119
Affinity Capture-MS Homo sapiens
135 RPS13 6207
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
136 Mis12  
Affinity Capture-MS Mus musculus
137 EGFR 1956
Affinity Capture-MS Homo sapiens
138 ING5  
Affinity Capture-MS Homo sapiens
139 RPL35A 6165
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
140 RPL12 6136
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
141 HP1BP3 50809
Affinity Capture-MS Homo sapiens
142 HNRNPAB 3182
Affinity Capture-MS Homo sapiens
143 RPL7L1 285855
Affinity Capture-MS Homo sapiens
144 NMD3 51068
Co-fractionation Homo sapiens
145 RPL18 6141
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
146 MAK16  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
147 RNF2  
Affinity Capture-MS Homo sapiens
148 BMI1  
Affinity Capture-MS Homo sapiens
149 ZC3HAV1 56829
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
150 RPL11 6135
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
151 HOMER1  
Affinity Capture-MS Homo sapiens
152 HSPA9 3313
Co-fractionation Homo sapiens
153 PABPC3 5042
Co-fractionation Homo sapiens
154 RPL26 6154
Affinity Capture-MS Homo sapiens
155 FTSJ3 117246
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
156 DOCK9 23348
Affinity Capture-MS Homo sapiens
157 RPS16 6217
Affinity Capture-MS Homo sapiens
158 PFAS 5198
Co-fractionation Homo sapiens
159 HK2 3099
Co-fractionation Homo sapiens
160 MARK3 4140
Affinity Capture-MS Homo sapiens
161 SURF6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
162 C8orf33 65265
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
163 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
164 ZNF689  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
165 NIP7 51388
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
166 SMG1 23049
Affinity Capture-MS Homo sapiens
167 RPL6 6128
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
168 TP53 7157
Negative Genetic Homo sapiens
169 RRS1 23212
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
170 NLE1 54475
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
171 ERH 2079
Affinity Capture-MS Homo sapiens
172 DFFA 1676
Co-fractionation Homo sapiens
173 KLF3  
Affinity Capture-MS Homo sapiens
174 KDM4C  
Affinity Capture-MS Homo sapiens
175 ZNF48  
Affinity Capture-MS Homo sapiens
176 NSA2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
177 DDX58 23586
Affinity Capture-RNA Homo sapiens
178 RPF1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
179 NIFK 84365
Affinity Capture-MS Homo sapiens
180 RPL34 6164
Affinity Capture-MS Homo sapiens
181 FKTN  
Affinity Capture-RNA Homo sapiens
182 ESR1  
Affinity Capture-MS Homo sapiens
183 RNF38  
Affinity Capture-MS Homo sapiens
184 TRIM37  
Proximity Label-MS Homo sapiens
185 EZH2  
Affinity Capture-MS Homo sapiens
186 HNRNPH3 3189
Co-fractionation Homo sapiens
187 YBX3 8531
Affinity Capture-MS Homo sapiens
188 TRIM31  
Affinity Capture-MS Homo sapiens
189 RPS8 6202
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
190 RPL8 6132
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
191 ZNF800  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
192 BRD1 23774
Affinity Capture-MS Homo sapiens
193 GLYR1 84656
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
194 SERBP1 26135
Affinity Capture-MS Homo sapiens
195 RPL23A 6147
Affinity Capture-MS Homo sapiens
196 AR 367
Proximity Label-MS Homo sapiens
197 RBM34  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
198 PURA 5813
Affinity Capture-MS Homo sapiens
199 REPIN1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
200 ZSCAN25  
Affinity Capture-MS Homo sapiens
201 RPL17 6139
Affinity Capture-MS Homo sapiens
202 LLPH  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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