Gene description for EIF2S3
Gene name eukaryotic translation initiation factor 2, subunit 3 gamma, 52kDa
Gene symbol EIF2S3
Other names/aliases EIF2
EIF2G
EIF2gamma
eIF-2gA
Species Homo sapiens
 Database cross references - EIF2S3
ExoCarta ExoCarta_1968
Vesiclepedia VP_1968
Entrez Gene 1968
HGNC 3267
MIM 300161
UniProt P41091  
 EIF2S3 identified in sEVs derived from the following tissue/cell type
B cells 20458337    
B cells 20458337    
B cells 20458337    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Chondrocytes 35931686    
Chondrocytes 35931686    
Colorectal cancer cells 25890246    
Colorectal cancer cells 25890246    
Colorectal cancer cells 25890246    
Colorectal cancer cells 34887515    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Foreskin fibroblasts 34108659    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Lymphoma cells 34108659    
Mammary cancer-associated fibroblasts 34108659    
Mesenchymal stem cells 36408942    
Monocytic leukemia cells 34108659    
Neuroblastoma cells 25944692    
Normal mammary epithelial cells 34108659    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic duct epithalial cells 34108659    
Pancreatic duct epithalial cells 34108659    
Pluripotent stem cells 34108659    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
T lymphocytes 34108659    
Thymus 23844026    
 Gene ontology annotations for EIF2S3
Molecular Function
    tRNA binding GO:0000049 IBA
    translation initiation factor activity GO:0003743 IBA
    translation initiation factor activity GO:0003743 IDA
    GTPase activity GO:0003924 TAS
    protein binding GO:0005515 IPI
    GTP binding GO:0005525 IEA
    translation factor activity, RNA binding GO:0008135 IDA
    cadherin binding GO:0045296 HDA
    membrane scission GTPase motor activity GO:1990606 IEA
    methionyl-initiator methionine tRNA binding GO:1990856 ISS
Biological Process
    formation of translation preinitiation complex GO:0001731 IBA
    cytoplasmic translational initiation GO:0002183 IMP
    translational initiation GO:0006413 IDA
Subcellular Localization
    nucleus GO:0005634 IDA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 TAS
    eukaryotic translation initiation factor 2 complex GO:0005850 IBA
    eukaryotic translation initiation factor 2 complex GO:0005850 IDA
    eukaryotic translation initiation factor 2 complex GO:0005850 IPI
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified EIF2S3 in sEVs
1
Experiment ID 79
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 1
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
2
Experiment ID 80
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis -Sample 2
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
3
Experiment ID 81
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 3
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
4
Experiment ID 489
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 490
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 491
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 492
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
8
Experiment ID 412
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MCF7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
9
Experiment ID 414
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
10
Experiment ID 426
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
11
Experiment ID 427
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
12
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
13
Experiment ID 498
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
14
Experiment ID 282
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors "Xu R, Greening DW, Rai A, Ji H, Simpson RJ."
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Ultracentrifugation - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
15
Experiment ID 283
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors "Xu R, Greening DW, Rai A, Ji H, Simpson RJ."
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Ultracentrifugation - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
16
Experiment ID 286
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors "Xu R, Greening DW, Rai A, Ji H, Simpson RJ."
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Sequential centrifugal ultrafiltration - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Sequential centrifugal ultrafiltration
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
17
Experiment ID 1203
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
18
Experiment ID 407
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
19
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
20
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
21
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
22
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
23
Experiment ID 405
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Foreskin fibroblasts
Sample name BJ
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
24
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
25
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
26
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
27
Experiment ID 417
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Lymphoma cells
Sample name Raji
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
28
Experiment ID 411
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Mammary cancer-associated fibroblasts
Sample name mCAF
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
29
Experiment ID 488
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
30
Experiment ID 418
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Monocytic leukemia cells
Sample name THP-1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
31
Experiment ID 224
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
32
Experiment ID 413
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Normal mammary epithelial cells
Sample name MCF10A
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
33
Experiment ID 211
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
34
Experiment ID 212
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
35
Experiment ID 406
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name BxPC3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
36
Experiment ID 415
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
37
Experiment ID 434
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
38
Experiment ID 435
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
39
Experiment ID 408
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic duct epithalial cells
Sample name HPDE
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
40
Experiment ID 409
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic duct epithalial cells
Sample name HPNE
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
41
Experiment ID 416
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pluripotent stem cells
Sample name PSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
42
Experiment ID 834
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
43
Experiment ID 835
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
44
Experiment ID 410
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample T lymphocytes
Sample name Jurkat
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
45
Experiment ID 217
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for EIF2S3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 EIF3A 8661
Affinity Capture-MS Homo sapiens
3 RPS12 6206
Affinity Capture-MS Homo sapiens
4 RIT1 6016
Negative Genetic Homo sapiens
5 EIF4G3 8672
Affinity Capture-MS Homo sapiens
6 RAD21 5885
Affinity Capture-Western Homo sapiens
7 RPL26L1 51121
Affinity Capture-MS Homo sapiens
8 RPS28 6234
Affinity Capture-MS Homo sapiens
9 SPRTN  
Affinity Capture-MS Homo sapiens
10 QARS 5859
Co-fractionation Homo sapiens
11 RPS3A 6189
Affinity Capture-MS Homo sapiens
12 SKIV2L 6499
Affinity Capture-MS Homo sapiens
13 EIF2S3 1968
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 RPL30 6156
Affinity Capture-MS Homo sapiens
15 RPS17 6218
Affinity Capture-MS Homo sapiens
16 TARDBP 23435
Affinity Capture-MS Homo sapiens
17 BTF3 689
Affinity Capture-MS Homo sapiens
18 EIF2B2 8892
Affinity Capture-MS Homo sapiens
19 SUB1 10923
Co-fractionation Homo sapiens
20 NOP56 10528
Affinity Capture-MS Homo sapiens
21 RPS19 6223
Affinity Capture-MS Homo sapiens
22 RPL10 6134
Affinity Capture-MS Homo sapiens
23 RPL14 9045
Affinity Capture-MS Homo sapiens
24 GTPBP1 9567
Co-fractionation Homo sapiens
25 EIF4A1 1973
Affinity Capture-MS Homo sapiens
26 CAND1 55832
Affinity Capture-MS Homo sapiens
27 SRP72 6731
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 EIF1 10209
Affinity Capture-MS Homo sapiens
29 PRKDC 5591
Co-fractionation Homo sapiens
30 RPL22 6146
Affinity Capture-MS Homo sapiens
31 RPS3 6188
Affinity Capture-MS Homo sapiens
32 ILF2 3608
Co-fractionation Homo sapiens
33 USP36  
Affinity Capture-MS Homo sapiens
34 RPS18 6222
Affinity Capture-MS Homo sapiens
35 VPS26A 9559
Co-fractionation Homo sapiens
36 CAPZB 832
Affinity Capture-MS Homo sapiens
37 RPL4 6124
Affinity Capture-MS Homo sapiens
38 RPL10A 4736
Affinity Capture-MS Homo sapiens
39 FBXW7  
Affinity Capture-MS Homo sapiens
40 TRIM28 10155
Co-fractionation Homo sapiens
41 RPL39 6170
Affinity Capture-MS Homo sapiens
42 RPL24 6152
Affinity Capture-MS Homo sapiens
43 CDC123 8872
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
44 NDRG1 10397
Affinity Capture-MS Homo sapiens
45 H1FX 8971
Affinity Capture-MS Homo sapiens
46 EIF2B4 8890
Affinity Capture-MS Homo sapiens
47 EIF3B 8662
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 RPLP1 6176
Affinity Capture-MS Homo sapiens
49 METAP2 10988
Affinity Capture-MS Homo sapiens
50 IMPDH2 3615
Co-fractionation Homo sapiens
51 IQGAP1 8826
Co-fractionation Homo sapiens
52 NPM1 4869
Affinity Capture-MS Homo sapiens
53 RPS8 6202
Affinity Capture-MS Homo sapiens
54 RPS9 6203
Affinity Capture-MS Homo sapiens
55 GSPT1 2935
Affinity Capture-MS Homo sapiens
56 EIF3E 3646
Affinity Capture-MS Homo sapiens
57 UGGT1 56886
Co-fractionation Homo sapiens
58 DST 667
Co-fractionation Homo sapiens
59 SRP68 6730
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
60 GNL3 26354
Affinity Capture-MS Homo sapiens
61 DNAJC7 7266
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
62 POLR2C 5432
Proximity Label-MS Homo sapiens
63 MTDH 92140
Affinity Capture-MS Homo sapiens
64 MYCN  
Affinity Capture-MS Homo sapiens
65 RBM42  
Affinity Capture-MS Homo sapiens
66 SERBP1 26135
Affinity Capture-MS Homo sapiens
67 RPS11 6205
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
68 PABPC4 8761
Affinity Capture-MS Homo sapiens
69 RPL19 6143
Affinity Capture-MS Homo sapiens
70 RBM8A 9939
Affinity Capture-MS Homo sapiens
71 LAMTOR1 55004
Proximity Label-MS Homo sapiens
72 EIF3C 8663
Affinity Capture-MS Homo sapiens
73 EIF1B  
Affinity Capture-MS Homo sapiens
74 UNK  
Affinity Capture-RNA Homo sapiens
75 E2F4  
Affinity Capture-MS Homo sapiens
76 RPS23 6228
Affinity Capture-MS Homo sapiens
77 NF2 4771
Affinity Capture-MS Homo sapiens
78 RPL27 6155
Affinity Capture-MS Homo sapiens
79 SRP9 6726
Affinity Capture-MS Homo sapiens
80 EIF3K 27335
Affinity Capture-MS Homo sapiens
81 RPL9 6133
Affinity Capture-MS Homo sapiens
82 ABCE1 6059
Affinity Capture-MS Homo sapiens
83 CD74 972
Affinity Capture-MS Homo sapiens
84 PARK2  
Affinity Capture-MS Homo sapiens
85 CCAR2 57805
Co-fractionation Homo sapiens
86 DRG1 4733
Affinity Capture-MS Homo sapiens
87 EIF2B5 8893
Affinity Capture-MS Homo sapiens
88 EIF2A 83939
Co-fractionation Homo sapiens
89 PSMD14 10213
Affinity Capture-MS Homo sapiens
90 RPL5 6125
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
91 ABCF2 10061
Co-fractionation Homo sapiens
92 PABPC1 26986
Affinity Capture-MS Homo sapiens
93 EEF1E1 9521
Co-fractionation Homo sapiens
94 GRAMD4 23151
Affinity Capture-MS Homo sapiens
95 C9orf72  
Affinity Capture-MS Homo sapiens
96 VCP 7415
Affinity Capture-MS Homo sapiens
97 NTRK1 4914
Affinity Capture-MS Homo sapiens
98 RPL31 6160
Affinity Capture-MS Homo sapiens
99 RPS7 6201
Affinity Capture-MS Homo sapiens
100 IFI16 3428
Affinity Capture-MS Homo sapiens
101 RPL13A 23521
Affinity Capture-MS Homo sapiens
102 DNAJA1 3301
Co-fractionation Homo sapiens
103 CDH1 999
Proximity Label-MS Homo sapiens
104 RPL36 25873
Affinity Capture-MS Homo sapiens
105 RPL27A 6157
Affinity Capture-MS Homo sapiens
106 DHFRL1  
Proximity Label-MS Homo sapiens
107 AIP 9049
Co-fractionation Homo sapiens
108 DHX9 1660
Affinity Capture-MS Homo sapiens
109 HECTD1 25831
Affinity Capture-MS Homo sapiens
110 ENY2 56943
Affinity Capture-MS Homo sapiens
111 RPLP0 6175
Affinity Capture-MS Homo sapiens
112 EIF3H 8667
Affinity Capture-MS Homo sapiens
113 C1orf131  
Affinity Capture-MS Homo sapiens
114 RPS15A 6210
Affinity Capture-MS Homo sapiens
115 Ksr1  
Affinity Capture-MS Mus musculus
116 CYLD  
Affinity Capture-MS Homo sapiens
117 RPL11 6135
Affinity Capture-MS Homo sapiens
118 LARP1 23367
Affinity Capture-MS Homo sapiens
119 CASP4  
Affinity Capture-MS Homo sapiens
120 PHLPP1  
Proximity Label-MS Homo sapiens
121 EIF3G 8666
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
122 SLX4  
Affinity Capture-MS Homo sapiens
123 RPL32 6161
Affinity Capture-MS Homo sapiens
124 FAM46D  
Affinity Capture-MS Homo sapiens
125 RBM39 9584
Affinity Capture-MS Homo sapiens
126 RABGGTA 5875
Co-fractionation Homo sapiens
127 RPLP2 6181
Affinity Capture-MS Homo sapiens
128 HBS1L 10767
Co-fractionation Homo sapiens
129 CDC73  
Affinity Capture-MS Homo sapiens
130 RPL7A 6130
Affinity Capture-MS Homo sapiens
131 MTIF2 4528
Co-fractionation Homo sapiens
132 RPS2 6187
Affinity Capture-MS Homo sapiens
133 XPO1 7514
Affinity Capture-MS Homo sapiens
134 EIF6 3692
Proximity Label-MS Homo sapiens
135 RPS20 6224
Affinity Capture-MS Homo sapiens
136 RPL35 11224
Affinity Capture-MS Homo sapiens
137 CD274 29126
Affinity Capture-MS Homo sapiens
138 CUL3 8452
Affinity Capture-MS Homo sapiens
139 RPS6 6194
Affinity Capture-MS Homo sapiens
140 RPL21 6144
Affinity Capture-MS Homo sapiens
141 RPL3 6122
Affinity Capture-MS Homo sapiens
142 EIF4E 1977
Affinity Capture-MS Homo sapiens
143 EIF2B3 8891
Affinity Capture-MS Homo sapiens
144 EIF2S2 8894
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
145 RPL7 6129
Affinity Capture-MS Homo sapiens
146 WIBG 84305
Co-fractionation Homo sapiens
147 ATXN2 6311
Affinity Capture-MS Homo sapiens
148 FN1 2335
Affinity Capture-MS Homo sapiens
149 GLUD1 2746
Affinity Capture-MS Homo sapiens
150 RPS27 6232
Affinity Capture-MS Homo sapiens
151 CDK2 1017
Affinity Capture-MS Homo sapiens
152 MAPK6  
Affinity Capture-MS Homo sapiens
153 NCAPH 23397
Affinity Capture-MS Homo sapiens
154 PSPC1 55269
Affinity Capture-MS Homo sapiens
155 RPA3 6119
Proximity Label-MS Homo sapiens
156 RPS13 6207
Affinity Capture-MS Homo sapiens
157 RPS14 6208
Affinity Capture-MS Homo sapiens
158 DCC  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
159 EIF3CL 728689
Affinity Capture-MS Homo sapiens
160 Bag2  
Affinity Capture-MS Mus musculus
161 RPL18 6141
Affinity Capture-MS Homo sapiens
162 DDRGK1 65992
Affinity Capture-MS Homo sapiens
163 RNF2  
Affinity Capture-MS Homo sapiens
164 KTN1 3895
Affinity Capture-MS Homo sapiens
165 RPS29 6235
Affinity Capture-MS Homo sapiens
166 EIF2B1 1967
Affinity Capture-MS Homo sapiens
167 RPS24 6229
Affinity Capture-MS Homo sapiens
168 AKAP1 8165
Proximity Label-MS Homo sapiens
169 ILF3 3609
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
170 XRCC5 7520
Co-fractionation Homo sapiens
171 EIF3D 8664
Affinity Capture-MS Homo sapiens
172 RPS16 6217
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
173 AIMP1 9255
Co-fractionation Homo sapiens
174 PA2G4 5036
Affinity Capture-MS Homo sapiens
175 RPL13 6137
Affinity Capture-MS Homo sapiens
176 EIF2S1 1965
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
177 GRK5 2869
Affinity Capture-MS Homo sapiens
178 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
179 PRRC2A 7916
Affinity Capture-MS Homo sapiens
180 RPS25 6230
Affinity Capture-MS Homo sapiens
181 PARP1 142
Proximity Label-MS Homo sapiens
182 BKRF1  
Affinity Capture-MS
183 HDGF 3068
Affinity Capture-MS Homo sapiens
184 RPL6 6128
Affinity Capture-MS Homo sapiens
185 POLR1B 84172
Co-fractionation Homo sapiens
186 RPS21 6227
Affinity Capture-MS Homo sapiens
187 EIF3I 8668
Affinity Capture-MS Homo sapiens
188 NUFIP2 57532
Affinity Capture-MS Homo sapiens
189 SRP19 6728
Affinity Capture-MS Homo sapiens
190 RPS4X 6191
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
191 FANCI 55215
Co-fractionation Homo sapiens
192 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
193 SRP54 6729
Affinity Capture-MS Homo sapiens
194 G3BP2 9908
Affinity Capture-MS Homo sapiens
195 RPS5 6193
Affinity Capture-MS Homo sapiens
196 ARF4 378
Proximity Label-MS Homo sapiens
197 RPL34 6164
Affinity Capture-MS Homo sapiens
198 RPS26 6231
Affinity Capture-MS Homo sapiens
199 ANAPC2 29882
Proximity Label-MS Homo sapiens
200 UFL1 23376
Affinity Capture-MS Homo sapiens
201 Hsph1 15505
Affinity Capture-MS Mus musculus
202 CEBPA  
Protein-peptide Homo sapiens
203 PLD3 23646
Co-fractionation Homo sapiens
204 RPL38 6169
Affinity Capture-MS Homo sapiens
205 IGF2BP3 10643
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
206 EIF3M 10480
Affinity Capture-MS Homo sapiens
207 ATG4B 23192
Affinity Capture-MS Homo sapiens
208 TRIM31  
Affinity Capture-MS Homo sapiens
209 Rrbp1  
Affinity Capture-MS Mus musculus
210 RPL8 6132
Affinity Capture-MS Homo sapiens
211 RPL28 6158
Affinity Capture-MS Homo sapiens
212 RPS27L 51065
Affinity Capture-MS Homo sapiens
213 XRCC6 2547
Co-fractionation Homo sapiens
Proximity Label-MS Homo sapiens
214 RPL23A 6147
Affinity Capture-MS Homo sapiens
215 KRAS 3845
Affinity Capture-MS Homo sapiens
Negative Genetic Homo sapiens
216 RARS 5917
Co-fractionation Homo sapiens
217 GNB2L1 10399
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
218 MYC  
Affinity Capture-MS Homo sapiens
219 PRMT1 3276
Affinity Capture-MS Homo sapiens
220 RPL17 6139
Affinity Capture-MS Homo sapiens
221 EIF5 1983
Affinity Capture-MS Homo sapiens
222 EIF3L 51386
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which EIF2S3 is involved
PathwayEvidenceSource
ABC-family proteins mediated transport TAS Reactome
Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S TAS Reactome
Antiviral mechanism by IFN-stimulated genes TAS Reactome
Cap-dependent Translation Initiation TAS Reactome
Cellular response to mitochondrial stress TAS Reactome
Cellular response to starvation IEA Reactome
Cellular responses to stimuli TAS Reactome
Cellular responses to stimuli IEA Reactome
Cellular responses to stress TAS Reactome
Cellular responses to stress IEA Reactome
Cytokine Signaling in Immune system TAS Reactome
Eukaryotic Translation Initiation TAS Reactome
Formation of the ternary complex, and subsequently, the 43S complex TAS Reactome
GTP hydrolysis and joining of the 60S ribosomal subunit TAS Reactome
Immune System TAS Reactome
Interferon Signaling TAS Reactome
L13a-mediated translational silencing of Ceruloplasmin expression TAS Reactome
Metabolism of proteins TAS Reactome
PERK regulates gene expression TAS Reactome
PERK regulates gene expression IEA Reactome
PKR-mediated signaling TAS Reactome
Recycling of eIF2:GDP TAS Reactome
Response of EIF2AK1 (HRI) to heme deficiency IEA Reactome
Response of EIF2AK4 (GCN2) to amino acid deficiency IEA Reactome
Ribosomal scanning and start codon recognition TAS Reactome
Translation TAS Reactome
Translation initiation complex formation TAS Reactome
Transport of small molecules TAS Reactome
Unfolded Protein Response (UPR) TAS Reactome
Unfolded Protein Response (UPR) IEA Reactome





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