Gene description for PRKRA
Gene name protein kinase, interferon-inducible double stranded RNA dependent activator
Gene symbol PRKRA
Other names/aliases DYT16
HSD14
PACT
RAX
Species Homo sapiens
 Database cross references - PRKRA
ExoCarta ExoCarta_8575
Entrez Gene 8575
HGNC 9438
MIM 603424
UniProt O75569  
 PRKRA identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Thymus 23844026    
 Gene ontology annotations for PRKRA
Molecular Function
    enzyme binding GO:0019899 IPI
    poly(A) RNA binding GO:0044822 IDA
    identical protein binding GO:0042802 IPI
    protein homodimerization activity GO:0042803 IPI
    protein binding GO:0005515 IPI
    double-stranded RNA binding GO:0003725 IDA
    enzyme activator activity GO:0008047 TAS
Biological Process
    pre-miRNA processing GO:0031054 IDA
    response to virus GO:0009615 TAS
    gene expression GO:0010467 TAS
    production of miRNAs involved in gene silencing by miRNA GO:0035196 IDA
    positive regulation of catalytic activity GO:0043085 TAS
    protein phosphorylation GO:0006468 IEA
    production of siRNA involved in RNA interference GO:0030422 IDA
    negative regulation of cell proliferation GO:0008285 TAS
    immune response GO:0006955 TAS
    positive regulation of intrinsic apoptotic signaling pathway GO:2001244 IEA
    middle ear morphogenesis GO:0042474 IEA
    skeletal system morphogenesis GO:0048705 IEA
    outer ear morphogenesis GO:0042473 IEA
    cellular response to oxidative stress GO:0034599 IEA
Subcellular Localization
    cytoplasm GO:0005737 IDA
    membrane GO:0016020 IDA
    nucleoplasm GO:0005654 IDA
    perinuclear region of cytoplasm GO:0048471 IEA
    cytosol GO:0005829 TAS
 Experiment description of studies that identified PRKRA in exosomes
1
Experiment ID 207
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
FLOT1
EV Membrane markers
VDAC
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 208
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
FLOT1
EV Membrane markers
VDAC
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 209
ISEV standards
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
FLOT1
EV Membrane markers
VDAC
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 211
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
cytochrome c|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 212
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
Cytochrome C|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 217
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
CD81|CD9|CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PRKRA
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RB1 5925
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
2 RPL36 25873
Affinity Capture-MS Homo sapiens
3 PRPSAP1 5635
Affinity Capture-MS Homo sapiens
4 EIF2AK2 5610
Invitro Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
5 IGF2BP1 10642
Affinity Capture-MS Homo sapiens
6 GDF9 2661
Two-hybrid Homo sapiens
7 SETDB1  
Two-hybrid Homo sapiens
8 COPS6 10980
Two-hybrid Homo sapiens
9 PRKRA 8575
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
10 TP53  
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
11 SYNCRIP 10492
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which PRKRA is involved
PathwayEvidenceSource
MicroRNA (miRNA) biogenesis TAS Reactome
Small interfering RNA (siRNA) biogenesis TAS Reactome





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