Gene description for MRPL20
Gene name mitochondrial ribosomal protein L20
Gene symbol MRPL20
Other names/aliases L20mt
MRP-L20
Species Homo sapiens
 Database cross references - MRPL20
ExoCarta ExoCarta_55052
Entrez Gene 55052
HGNC 14478
MIM 611833
UniProt Q9BYC9  
 MRPL20 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for MRPL20
Molecular Function
    rRNA binding GO:0019843 IEA
    structural constituent of ribosome GO:0003735 IEA
    protein binding GO:0005515 IPI
    poly(A) RNA binding GO:0044822 IDA
Biological Process
    mitochondrial translational initiation GO:0070124 TAS
    mitochondrial translational elongation GO:0070125 TAS
    organelle organization GO:0006996 TAS
    mitochondrial translational termination GO:0070126 TAS
    mitochondrial translation GO:0032543 TAS
Subcellular Localization
    mitochondrial ribosome GO:0005761 IDA
    mitochondrial inner membrane GO:0005743 TAS
 Experiment description of studies that identified MRPL20 in exosomes
1
Experiment ID 235
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 236
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for MRPL20
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CAPZB 832
Two-hybrid Homo sapiens
View the network image/svg+xml



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