Gene description for SMAD5
Gene name
SMAD family member 5
Gene symbol
SMAD5
Other names/aliases
DWFC JV5-1 MADH5
Species
Homo sapiens
Database cross references - SMAD5
ExoCarta
ExoCarta_4090
Entrez Gene
4090
HGNC
6771
MIM
603110
UniProt
Q99717
SMAD5 identified in exosomes derived from the following tissue/cell type
Ovarian cancer cells
23333927
Ovarian cancer cells
23333927
Gene ontology annotations for SMAD5
Experiment description of studies that identified SMAD5 in exosomes
1
Experiment ID
211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23333927
Organism
Homo sapiens
Experiment description
Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors
Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name
J Proteomics
Publication year
2013
Sample
Ovarian cancer cells
Sample name
IGROV1
Isolation/purification methods
Differential centrifugation Ultracentrifugation Sucrose density gradient
Flotation density
1.09-1.15 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
2
Experiment ID
212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23333927
Organism
Homo sapiens
Experiment description
Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors
Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name
J Proteomics
Publication year
2013
Sample
Ovarian cancer cells
Sample name
OVCAR-3
Isolation/purification methods
Differential centrifugation Ultracentrifugation Sucrose density gradient
Flotation density
1.09-1.15 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
Protein-protein interactions for SMAD5
Protein Interactor
ExoCarta ID
Identification method
PubMed
Species
1
PPIL4
Affinity Capture-MS
Homo sapiens
2
ACVR1
90
Invivo
Homo sapiens
Invivo
Homo sapiens
Affinity Capture-MS
Homo sapiens
3
CHMP3
51652
Affinity Capture-MS
Homo sapiens
4
RBM4
5936
Affinity Capture-MS
Homo sapiens
5
SNRPA
6626
Affinity Capture-MS
Homo sapiens
6
TOB1
Affinity Capture-Western
Homo sapiens
Affinity Capture-MS
Homo sapiens
7
SMAD1
Affinity Capture-MS
Homo sapiens
8
SMAD4
Affinity Capture-MS
Homo sapiens
9
BCAT1
586
Affinity Capture-MS
Homo sapiens
10
PNKP
11284
Affinity Capture-MS
Homo sapiens
11
CXXC5
Affinity Capture-MS
Homo sapiens
12
FLNA
Invivo
Homo sapiens
Invitro
Homo sapiens
Two-hybrid
Homo sapiens
13
ZNF8
Invitro
Homo sapiens
Invivo
Homo sapiens
Two-hybrid
Homo sapiens
14
U2AF2
11338
Affinity Capture-MS
Homo sapiens
15
WWP1
11059
Affinity Capture-MS
Homo sapiens
16
ZFHX1B
Invivo
Homo sapiens
Invitro
Homo sapiens
Two-hybrid
Homo sapiens
View the network
image/svg+xml
Pathways in which SMAD5 is involved