Gene description for BTRC
Gene name beta-transducin repeat containing E3 ubiquitin protein ligase
Gene symbol BTRC
Other names/aliases BETA-TRCP
FBW1A
FBXW1
FBXW1A
FWD1
bTrCP
bTrCP1
betaTrCP
Species Homo sapiens
 Database cross references - BTRC
ExoCarta ExoCarta_8945
Entrez Gene 8945
HGNC 1144
MIM 603482
UniProt Q9Y297  
 BTRC identified in exosomes derived from the following tissue/cell type
Hepatocytes 26054723    
 Gene ontology annotations for BTRC
Molecular Function
    protein dimerization activity GO:0046983 IEA
    ubiquitin-protein transferase activity GO:0004842 IDA
    ligase activity GO:0016874 IEA
    beta-catenin binding GO:0008013 IEA
    protein binding GO:0005515 IPI
    protein phosphorylated amino acid binding GO:0045309 IEA
Biological Process
    regulation of proteasomal protein catabolic process GO:0061136 IEA
    branching involved in mammary gland duct morphogenesis GO:0060444 IEA
    ubiquitin-dependent protein catabolic process GO:0006511 IDA
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 IDA
    cellular response to organic cyclic compound GO:0071407 IEA
    mammary gland epithelial cell proliferation GO:0033598 IEA
    circadian rhythm GO:0007623 TAS
    mitotic cell cycle GO:0000278 TAS
    G2/M transition of mitotic cell cycle GO:0000086 TAS
    negative regulation of smoothened signaling pathway GO:0045879 TAS
    Wnt signaling pathway GO:0016055 IEA
    positive regulation of circadian rhythm GO:0042753 ISS
    regulation of circadian rhythm GO:0042752 IDA
    positive regulation of proteolysis GO:0045862 IMP
    negative regulation of transcription, DNA-templated GO:0045892 IMP
    regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle GO:0051439 TAS
    positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition GO:0051437 TAS
    innate immune response GO:0045087 TAS
    positive regulation of transcription, DNA-templated GO:0045893 ISS
    regulation of I-kappaB kinase/NF-kappaB signaling GO:0043122 IEA
    protein ubiquitination GO:0016567 IDA
    protein polyubiquitination GO:0000209 IEA
    SCF-dependent proteasomal ubiquitin-dependent protein catabolic process GO:0031146 IBA
    protein dephosphorylation GO:0006470 ISS
    viral process GO:0016032 TAS
    anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process GO:0031145 TAS
    negative regulation of sequence-specific DNA binding transcription factor activity GO:0043433 TAS
    protein destabilization GO:0031648 IMP
    stimulatory C-type lectin receptor signaling pathway GO:0002223 TAS
    signal transduction GO:0007165 TAS
Subcellular Localization
    nucleoplasm GO:0005654 TAS
    SCF ubiquitin ligase complex GO:0019005 IDA
    cytosol GO:0005829 TAS
 Experiment description of studies that identified BTRC in exosomes
1
Experiment ID 237
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for BTRC
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CUL1 8454
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
2 BTRC 8945
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
3 FBXW11  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
4 JUP 3728
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
5 SUFU  
Affinity Capture-MS Homo sapiens
6 UBQLN2 29978
Invivo Homo sapiens
7 CTNNB1 1499
Invitro Homo sapiens
Invivo Homo sapiens
8 NFKBIA  
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
9 DLG1 1739
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
10 SKP1 6500
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
11 RELA  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
12 SMAD4  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
13 TRIM9  
Two-hybrid Homo sapiens
14 UBE2R2 54926
Reconstituted Complex Homo sapiens
15 DLG7  
Affinity Capture-MS Homo sapiens
16 TBK1 29110
Affinity Capture-MS Homo sapiens
17 SMAD3  
Affinity Capture-Western Homo sapiens
18 CD4 920
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
19 NFKB1 4790
Affinity Capture-Western Homo sapiens
20 NFKBIB  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
21 NFKB2 4791
Affinity Capture-Western Homo sapiens
22 IKBKB 3551
Affinity Capture-Western Homo sapiens
23 CDC34  
Reconstituted Complex Homo sapiens
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