Gene description for NFIA
Gene name nuclear factor I/A
Gene symbol NFIA
Other names/aliases CTF
NF-I/A
NF1-A
NFI-A
NFI-L
Species Homo sapiens
 Database cross references - NFIA
ExoCarta ExoCarta_4774
Vesiclepedia VP_4774
Entrez Gene 4774
HGNC 7784
MIM 600727
UniProt Q12857  
 NFIA identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for NFIA
Molecular Function
    RNA polymerase II cis-regulatory region sequence-specific DNA binding GO:0000978 IBA
    RNA polymerase II cis-regulatory region sequence-specific DNA binding GO:0000978 IDA
    DNA-binding transcription factor activity, RNA polymerase II-specific GO:0000981 IBA
    DNA-binding transcription factor activity, RNA polymerase II-specific GO:0000981 ISA
    DNA-binding transcription activator activity, RNA polymerase II-specific GO:0001228 IDA
    chromatin binding GO:0003682 IEA
    DNA-binding transcription factor activity GO:0003700 NAS
    protein binding GO:0005515 IPI
    DNA-binding transcription factor binding GO:0140297 IPI
Biological Process
    cell morphogenesis GO:0000902 IEA
    DNA replication GO:0006260 IEA
    regulation of DNA-templated transcription GO:0006355 NAS
    regulation of transcription by RNA polymerase II GO:0006357 IBA
    response to wounding GO:0009611 IEA
    exit from mitosis GO:0010458 IEA
    gene expression GO:0010467 IEA
    glial cell proliferation GO:0014009 IEA
    viral genome replication GO:0019079 NAS
    glial cell fate specification GO:0021780 IEA
    BMP signaling pathway GO:0030509 IEA
    limb morphogenesis GO:0035108 IEA
    positive regulation of transcription by RNA polymerase II GO:0045944 IDA
    neuron fate specification GO:0048665 IEA
    cartilage development GO:0051216 IEA
    retina development in camera-type eye GO:0060041 IEA
    synapse maturation GO:0060074 IEA
    neural precursor cell proliferation GO:0061351 IEA
    ureter development GO:0072189 IEA
Subcellular Localization
    chromatin GO:0000785 ISA
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleus GO:0005634 NAS
    nucleoplasm GO:0005654 IDA
    cell junction GO:0030054 IDA
 Experiment description of studies that identified NFIA in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for NFIA
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GABARAPL1 23710
Two-hybrid Homo sapiens
2 FOXC1  
Proximity Label-MS Homo sapiens
3 BCL9  
Proximity Label-MS Homo sapiens
4 ZNF281  
Proximity Label-MS Homo sapiens
5 UTP14A 10813
Co-fractionation Homo sapiens
6 ELF1 1997
Proximity Label-MS Homo sapiens
7 IRF4  
Proximity Label-MS Homo sapiens
8 COIL  
Proximity Label-MS Homo sapiens
9 FEV  
Proximity Label-MS Homo sapiens
10 NFIB  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 BCOR  
Proximity Label-MS Homo sapiens
12 AES 166
Proximity Label-MS Homo sapiens
13 LHX2  
Proximity Label-MS Homo sapiens
Reconstituted Complex Homo sapiens
14 HIST1H2BG 8339
Proximity Label-MS Homo sapiens
15 GATA2  
Proximity Label-MS Homo sapiens
Reconstituted Complex Homo sapiens
16 EN1  
Proximity Label-MS Homo sapiens
17 NOP56 10528
Co-fractionation Homo sapiens
18 PAX2  
Proximity Label-MS Homo sapiens
19 SURF4 6836
Co-fractionation Homo sapiens
20 TCF7L2  
Proximity Label-MS Homo sapiens
21 TLE3  
Proximity Label-MS Homo sapiens
22 UBC 7316
Reconstituted Complex Homo sapiens
23 KDM6A  
Proximity Label-MS Homo sapiens
24 TRIM55  
Co-fractionation Homo sapiens
25 ARID3B  
Proximity Label-MS Homo sapiens
26 SOX9  
Proximity Label-MS Homo sapiens
27 SOX10  
Proximity Label-MS Homo sapiens
28 SOX2  
Proximity Label-MS Homo sapiens
29 MNX1  
Proximity Label-MS Homo sapiens
30 MYOD1  
Proximity Label-MS Homo sapiens
31 SATB2  
Proximity Label-MS Homo sapiens
32 QSER1  
Proximity Label-MS Homo sapiens
33 HOXB9  
Proximity Label-MS Homo sapiens
34 RPS6 6194
Co-fractionation Homo sapiens
35 NCOR1  
Proximity Label-MS Homo sapiens
36 ZNF148  
Proximity Label-MS Homo sapiens
37 GSE1 23199
Proximity Label-MS Homo sapiens
38 KLF8  
Proximity Label-MS Homo sapiens
Reconstituted Complex Homo sapiens
39 TBR1  
Proximity Label-MS Homo sapiens
40 E2F6 1876
Proximity Label-MS Homo sapiens
41 TRA2B 6434
Co-fractionation Homo sapiens
42 ARID4B  
Proximity Label-MS Homo sapiens
43 KMT2D 8085
Proximity Label-MS Homo sapiens
44 WIZ 58525
Proximity Label-MS Homo sapiens
45 GCM1  
Proximity Label-MS Homo sapiens
46 NCOA3  
Proximity Label-MS Homo sapiens
47 TEAD1  
Proximity Label-MS Homo sapiens
48 PCDHA2  
Co-fractionation Homo sapiens
49 HMG20A  
Proximity Label-MS Homo sapiens
50 KLF4  
Proximity Label-MS Homo sapiens
51 NR2C1  
Proximity Label-MS Homo sapiens
52 SFN 2810
Reconstituted Complex Homo sapiens
53 LHX4  
Proximity Label-MS Homo sapiens
54 SOX5 6660
Proximity Label-MS Homo sapiens
55 RRP7A 27341
Co-fractionation Homo sapiens
56 RBPJ 3516
Affinity Capture-MS Homo sapiens
57 ACIN1 22985
Co-fractionation Homo sapiens
58 FOXP1 27086
Proximity Label-MS Homo sapiens
59 DPF1  
Proximity Label-MS Homo sapiens
60 KLF16  
Proximity Label-MS Homo sapiens
Reconstituted Complex Homo sapiens
61 XPO1 7514
Affinity Capture-MS Homo sapiens
62 FOXA1  
Proximity Label-MS Homo sapiens
63 PRDM1  
Proximity Label-MS Homo sapiens
64 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
65 DDX21 9188
Co-fractionation Homo sapiens
66 TLX3  
Proximity Label-MS Homo sapiens
67 SSBP1 6742
Proximity Label-MS Homo sapiens
68 NCOR2  
Proximity Label-MS Homo sapiens
69 HIVEP1 3096
Proximity Label-MS Homo sapiens
70 TRA2A 29896
Co-fractionation Homo sapiens
71 NFIX 4784
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
72 GOLGA2 2801
Proximity Label-MS Homo sapiens
73 NOP2 4839
Co-fractionation Homo sapiens
74 SMARCD2 6603
Proximity Label-MS Homo sapiens
75 GATA3  
Proximity Label-MS Homo sapiens
Reconstituted Complex Homo sapiens
76 DPF2  
Proximity Label-MS Homo sapiens
77 SMAD3 4088
Reconstituted Complex Homo sapiens
78 LHX3  
Proximity Label-MS Homo sapiens
Reconstituted Complex Homo sapiens
79 DTX2 113878
Proximity Label-MS Homo sapiens
80 TPBG 7162
Co-fractionation Homo sapiens
81 PAX8  
Proximity Label-MS Homo sapiens
82 SMAD2 4087
Reconstituted Complex Homo sapiens
83 RPL37A 6168
Co-fractionation Homo sapiens
84 CIC 23152
Proximity Label-MS Homo sapiens
85 DHX9 1660
Co-fractionation Homo sapiens
86 PAXIP1  
Proximity Label-MS Homo sapiens
87 DYNLL2 140735
Proximity Label-MS Homo sapiens
88 T  
Proximity Label-MS Homo sapiens
Reconstituted Complex Homo sapiens
89 SON 6651
Co-fractionation Homo sapiens
90 KLF12  
Proximity Label-MS Homo sapiens
91 SOX17  
Proximity Label-MS Homo sapiens
92 ZNF609  
Proximity Label-MS Homo sapiens
93 VSX1  
Proximity Label-MS Homo sapiens
94 GPS2  
Proximity Label-MS Homo sapiens
95 BCL7C  
Proximity Label-MS Homo sapiens
96 SSBP3  
Proximity Label-MS Homo sapiens
97 ZNF326 284695
Co-fractionation Homo sapiens
98 ATXN1 6310
Proximity Label-MS Homo sapiens
99 ZNF644  
Proximity Label-MS Homo sapiens
100 TLX2  
Proximity Label-MS Homo sapiens
101 PAX6  
Proximity Label-MS Homo sapiens
102 PLEKHF2 79666
Two-hybrid Homo sapiens
103 SOX6  
Proximity Label-MS Homo sapiens
104 SMARCD1 6602
Proximity Label-MS Homo sapiens
105 ELK3  
Proximity Label-MS Homo sapiens
Reconstituted Complex Homo sapiens
106 RLIM 51132
Affinity Capture-MS Homo sapiens
107 MAML1  
Proximity Label-MS Homo sapiens
108 FOXI1  
Proximity Label-MS Homo sapiens
109 TFAP4 7023
Affinity Capture-MS Homo sapiens
110 NR3C1 2908
Proximity Label-MS Homo sapiens
111 ADNP 23394
Co-fractionation Homo sapiens
112 FOXC2  
Affinity Capture-MS Homo sapiens
113 YY1 7528
Proximity Label-MS Homo sapiens
114 FOXL1  
Proximity Label-MS Homo sapiens
Reconstituted Complex Homo sapiens
115 ELMSAN1  
Proximity Label-MS Homo sapiens
116 SOX15  
Proximity Label-MS Homo sapiens
117 HNF4A  
Proximity Label-MS Homo sapiens
118 FOS 2353
Proximity Label-MS Homo sapiens
Reconstituted Complex Homo sapiens
119 MYC  
Affinity Capture-MS Homo sapiens
120 IRF8  
Proximity Label-MS Homo sapiens
121 FOXP4  
Proximity Label-MS Homo sapiens
122 TBL1X 6907
Proximity Label-MS Homo sapiens
123 HP1BP3 50809
Co-fractionation Homo sapiens
124 ETV3  
Proximity Label-MS Homo sapiens
125 SRSF10 10772
Co-fractionation Homo sapiens
126 RPS11 6205
Co-fractionation Homo sapiens
127 SMARCA2 6595
Proximity Label-MS Homo sapiens
128 ARID1A 8289
Proximity Label-MS Homo sapiens
129 MYB  
Proximity Label-MS Homo sapiens
130 FTSJ3 117246
Co-fractionation Homo sapiens
131 KDM2B 84678
Proximity Label-MS Homo sapiens
132 TLX1  
Proximity Label-MS Homo sapiens
133 PARP1 142
Proximity Label-MS Homo sapiens
134 TRPS1  
Proximity Label-MS Homo sapiens
135 RRS1 23212
Co-fractionation Homo sapiens
136 RBM25 58517
Co-fractionation Homo sapiens
137 ARID1B 57492
Proximity Label-MS Homo sapiens
138 ETV7  
Proximity Label-MS Homo sapiens
Reconstituted Complex Homo sapiens
139 SAP18 10284
Co-fractionation Homo sapiens
140 KLF3  
Proximity Label-MS Homo sapiens
Reconstituted Complex Homo sapiens
141 TLE1 7088
Proximity Label-MS Homo sapiens
142 NCOA2 10499
Proximity Label-MS Homo sapiens
143 SIX4  
Proximity Label-MS Homo sapiens
144 SMAD4  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
145 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
146 CREBBP  
Proximity Label-MS Homo sapiens
147 PAX9  
Proximity Label-MS Homo sapiens
148 CREB1  
Affinity Capture-MS Homo sapiens
149 PRR12 57479
Proximity Label-MS Homo sapiens
150 VTN 7448
Co-fractionation Homo sapiens
151 KLF5  
Proximity Label-MS Homo sapiens
152 ATXN1L  
Proximity Label-MS Homo sapiens
153 PAX7  
Proximity Label-MS Homo sapiens
154 RPS24 6229
Co-fractionation Homo sapiens
155 ZNF608  
Proximity Label-MS Homo sapiens
156 SRSF7 6432
Co-fractionation Homo sapiens
157 CIT 11113
Affinity Capture-MS Homo sapiens
158 ERG  
Proximity Label-MS Homo sapiens
Reconstituted Complex Homo sapiens
159 EHMT1  
Proximity Label-MS Homo sapiens
160 ELF2  
Proximity Label-MS Homo sapiens
161 HNF1B  
Proximity Label-MS Homo sapiens
162 HNRNPU 3192
Co-fractionation Homo sapiens
163 NFIC 4782
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
164 AR 367
Proximity Label-MS Homo sapiens
165 ARID3A  
Proximity Label-MS Homo sapiens
166 IRF2BP2  
Proximity Label-MS Homo sapiens
167 SP7  
Proximity Label-MS Homo sapiens
168 MED15  
Proximity Label-MS Homo sapiens
169 DPY30 84661
Proximity Label-MS Homo sapiens
170 SRSF3 6428
Co-fractionation Homo sapiens
171 LUC7L3 51747
Co-fractionation Homo sapiens
172 EP300 2033
Proximity Label-MS Homo sapiens
173 NHLH1  
Proximity Label-MS Homo sapiens
Reconstituted Complex Homo sapiens
174 RPS13 6207
Co-fractionation Homo sapiens
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