Gene description for GGA1
Gene name golgi-associated, gamma adaptin ear containing, ARF binding protein 1
Gene symbol GGA1
Other names/aliases -
Species Homo sapiens
 Database cross references - GGA1
ExoCarta ExoCarta_26088
Vesiclepedia VP_26088
Entrez Gene 26088
HGNC 17842
MIM 606004
UniProt Q9UJY5  
 GGA1 identified in exosomes derived from the following tissue/cell type
Neuroblastoma cells 25944692    
 Gene ontology annotations for GGA1
Molecular Function
    protein binding GO:0005515 IPI
    small GTPase binding GO:0031267 IBA
    small GTPase binding GO:0031267 IPI
    phosphatidylinositol binding GO:0035091 IEA
    ubiquitin binding GO:0043130 IEA
Biological Process
    intracellular protein transport GO:0006886 IDA
    intracellular protein transport GO:0006886 TAS
    Golgi to plasma membrane transport GO:0006893 IBA
    protein localization GO:0008104 IDA
    protein catabolic process GO:0030163 IEA
    protein localization to cell surface GO:0034394 IBA
    protein localization to cell surface GO:0034394 IDA
    retrograde transport, endosome to Golgi GO:0042147 IDA
    Golgi to plasma membrane protein transport GO:0043001 IDA
    positive regulation of protein catabolic process GO:0045732 IEA
    protein localization to ciliary membrane GO:1903441 IEA
Subcellular Localization
    nucleoplasm GO:0005654 IDA
    early endosome GO:0005769 IDA
    Golgi apparatus GO:0005794 IDA
    Golgi apparatus GO:0005794 TAS
    trans-Golgi network GO:0005802 IBA
    cytosol GO:0005829 IEA
    endosome membrane GO:0010008 TAS
    membrane GO:0016020 HDA
    early endosome membrane GO:0031901 TAS
    protein-containing complex GO:0032991 IMP
    intracellular membrane-bounded organelle GO:0043231 IDA
 Experiment description of studies that identified GGA1 in exosomes
1
Experiment ID 224
MISEV standards
EM|AFM
Biophysical techniques
Alix|TSG101|CD63|CD81
Enriched markers
GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for GGA1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 LRP3  
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
2 STAB1  
Reconstituted Complex Homo sapiens
3 SORL1 6653
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Protein-peptide Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
4 ATG9A 79065
Proximity Label-MS Homo sapiens
5 Adipoq  
Reconstituted Complex Mus musculus
6 CCDC91 55297
FRET Homo sapiens
Co-crystal Structure Homo sapiens
Protein-peptide Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 TSG101 7251
Two-hybrid Homo sapiens
8 ARF3 377
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Proximity Label-MS Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
9 TMEM9B 56674
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 UBC 7316
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
11 CSNK2A1 1457
Biochemical Activity Homo sapiens
12 CLTB 1212
Proximity Label-MS Homo sapiens
13 APP 351
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
14 LRP10 26020
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 NDE1 54820
Affinity Capture-MS Homo sapiens
16 MON2 23041
Affinity Capture-Western Homo sapiens
17 AP1G1 164
Affinity Capture-Western Homo sapiens
18 CCDC53 51019
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 GGA1 26088
Protein-peptide Homo sapiens
Co-crystal Structure Homo sapiens
Protein-peptide Homo sapiens
Co-crystal Structure Homo sapiens
20 TOM1L1 10040
Co-fractionation Homo sapiens
21 LIX1L 128077
Co-fractionation Homo sapiens
22 TMEM59 9528
Affinity Capture-MS Homo sapiens
23 EXOC5 10640
Affinity Capture-MS Homo sapiens
24 RNF11 26994
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Protein-peptide Homo sapiens
25 C1orf216  
Two-hybrid Homo sapiens
26 RABGEF1 27342
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
27 TOLLIP 54472
Reconstituted Complex Homo sapiens
28 CENPH  
Affinity Capture-MS Homo sapiens
29 PSMD4 5710
Affinity Capture-MS Homo sapiens
30 CLCN3 1182
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 RABEP1 9135
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Co-crystal Structure Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Co-crystal Structure Homo sapiens
Protein-peptide Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
32 TFDP1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 EXOC4 60412
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 RAB4A 5867
Proximity Label-MS Homo sapiens
35 SNAP47 116841
Affinity Capture-MS Homo sapiens
36 DTNBP1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 NECAP2 55707
Affinity Capture-Western Homo sapiens
38 PCCA 5095
Co-fractionation Homo sapiens
39 HAUS8  
Affinity Capture-MS Homo sapiens
40 VCP 7415
Affinity Capture-MS Homo sapiens
41 DTNB 1838
Two-hybrid Homo sapiens
42 BACE2 25825
Protein-peptide Homo sapiens
Reconstituted Complex Homo sapiens
43 SMAD3 4088
Two-hybrid Homo sapiens
44 C17orf70  
Affinity Capture-MS Homo sapiens
45 FLNB 2317
Co-fractionation Homo sapiens
46 DNM2 1785
Co-fractionation Homo sapiens
47 EIF4EBP1  
Co-fractionation Homo sapiens
48 CEP72  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 VPS18 57617
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
50 F8 2157
Two-hybrid Homo sapiens
51 STAM 8027
Co-fractionation Homo sapiens
52 BAG3 9531
Affinity Capture-Luminescence Homo sapiens
53 CLINT1 9685
Protein-peptide Homo sapiens
54 RAB5A 5868
Proximity Label-MS Homo sapiens
55 E2F5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 IGF2R 281849
Reconstituted Complex Bos taurus
57 IGF2R 3482
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Co-crystal Structure Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
58 ARF6 382
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
59 RABGAP1 23637
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
60 PPP2CA 282320
Biochemical Activity Bos taurus
61 AKT2 208
Co-fractionation Homo sapiens
62 RABGAP1L 9910
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
63 BICD1 636
Proximity Label-MS Homo sapiens
64 CLTC 1213
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Co-crystal Structure Homo sapiens
Reconstituted Complex Homo sapiens
65 DNAJC13 23317
Affinity Capture-MS Homo sapiens
66 SYNRG 11276
Two-hybrid Homo sapiens
Protein-peptide Homo sapiens
Protein-peptide Homo sapiens
Affinity Capture-Western Homo sapiens
67 DHX15 1665
Co-fractionation Homo sapiens
68 GGA3 23163
Reconstituted Complex Homo sapiens
Co-localization Homo sapiens
Reconstituted Complex Homo sapiens
69 RABEPK  
Co-fractionation Homo sapiens
70 GIPC1 10755
Co-fractionation Homo sapiens
71 TMEM9 252839
Affinity Capture-MS Homo sapiens
72 CDKL3  
Two-hybrid Homo sapiens
73 MAP1S 55201
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
74 BACE1  
FRET Homo sapiens
Affinity Capture-Western Homo sapiens
Co-crystal Structure Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
FRET Homo sapiens
Reconstituted Complex Homo sapiens
Co-crystal Structure Homo sapiens
Affinity Capture-Western Homo sapiens
75 M6PR 4074
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Protein-peptide Homo sapiens
76 ARF5 381
Reconstituted Complex Homo sapiens
77 SQSTM1 8878
Proximity Label-MS Homo sapiens
78 PCMT1 5110
Cross-Linking-MS (XL-MS) Homo sapiens
79 Arf1 11840
Co-crystal Structure Mus musculus
80 SLC38A7 55238
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
81 ECHDC1 55862
Affinity Capture-MS Homo sapiens
82 CEP44  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
83 SORT1 6272
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Protein-peptide Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
84 CLTA 1211
Proximity Label-MS Homo sapiens
85 ARF1 375
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
86 GGA2 23062
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-localization Homo sapiens
Reconstituted Complex Homo sapiens
87 MAP1A 4130
Affinity Capture-MS Homo sapiens
88 CDKN2A 1029
Two-hybrid Homo sapiens
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