Gene description for CDC16
Gene name cell division cycle 16
Gene symbol CDC16
Other names/aliases ANAPC6
APC6
CUT9
Species Homo sapiens
 Database cross references - CDC16
ExoCarta ExoCarta_8881
Entrez Gene 8881
HGNC 1720
MIM 603461
UniProt Q13042  
 CDC16 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
 Gene ontology annotations for CDC16
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    cell division GO:0051301 IEA
    regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle GO:0051439 TAS
    mitotic cell cycle GO:0000278 TAS
    mitotic spindle assembly checkpoint GO:0007094 TAS
    positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition GO:0051437 TAS
    mitotic nuclear division GO:0007067 IEA
    anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process GO:0031145 TAS
    regulation of mitotic nuclear division GO:0007088 TAS
    cell proliferation GO:0008283 TAS
    negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle GO:0051436 TAS
    protein K11-linked ubiquitination GO:0070979 IDA
Subcellular Localization
    spindle GO:0005819 TAS
    centrosome GO:0005813 IDA
    spindle microtubule GO:0005876 IDA
    nucleoplasm GO:0005654 TAS
    cytosol GO:0005829 TAS
    anaphase-promoting complex GO:0005680 IDA
    cytoplasm GO:0005737 IDA
 Experiment description of studies that identified CDC16 in exosomes
1
Experiment ID 207
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
FLOT1
EV Membrane markers
VDAC
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 208
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
FLOT1
EV Membrane markers
VDAC
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 209
ISEV standards
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
FLOT1
EV Membrane markers
VDAC
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for CDC16
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CDC20  
Invivo Homo sapiens
Invitro Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
2 ANAPC4  
Affinity Capture-Western Homo sapiens
3 APC2  
Affinity Capture-Western Homo sapiens
4 ANAPC5 51433
Invivo Homo sapiens
5 ANAPC1 64682
Co-purification Homo sapiens
6 Ppp5c  
Two-hybrid Mus musculus
Two-hybrid Mus musculus
7 BUB1B  
Invivo Homo sapiens
8 CDH1 999
Reconstituted Complex Homo sapiens
9 MAD2L1  
Invitro Homo sapiens
Invivo Homo sapiens
10 SMAD3  
Affinity Capture-Western Homo sapiens
11 SMAD2 4087
Affinity Capture-Western Homo sapiens
12 CDC27 996
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
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