Gene description for MED24
Gene name mediator complex subunit 24
Gene symbol MED24
Other names/aliases ARC100
CRSP100
CRSP4
DRIP100
THRAP4
TRAP100
Species Homo sapiens
 Database cross references - MED24
ExoCarta ExoCarta_9862
Vesiclepedia VP_9862
Entrez Gene 9862
HGNC 22963
MIM 607000
UniProt O75448  
 MED24 identified in sEVs derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
 Gene ontology annotations for MED24
Molecular Function
    transcription coregulator activity GO:0003712 IBA
    transcription coregulator activity GO:0003712 IDA
    transcription coactivator activity GO:0003713 IDA
    protein binding GO:0005515 IPI
    nuclear receptor coactivator activity GO:0030374 NAS
    nuclear vitamin D receptor binding GO:0042809 NAS
    nuclear thyroid hormone receptor binding GO:0046966 IDA
Biological Process
    positive regulation of transcription elongation by RNA polymerase II GO:0032968 NAS
    positive regulation of DNA-templated transcription GO:0045893 IDA
    RNA polymerase II preinitiation complex assembly GO:0051123 NAS
    positive regulation of transcription initiation by RNA polymerase II GO:0060261 IBA
    positive regulation of transcription initiation by RNA polymerase II GO:0060261 IDA
    positive regulation of transcription initiation by RNA polymerase II GO:0060261 NAS
Subcellular Localization
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 TAS
    mediator complex GO:0016592 IBA
    mediator complex GO:0016592 NAS
    core mediator complex GO:0070847 IPI
 Experiment description of studies that identified MED24 in sEVs
1
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for MED24
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SUCO  
Affinity Capture-MS Homo sapiens
2 Mis12  
Affinity Capture-MS Mus musculus
3 SERPINB4 6318
Affinity Capture-MS Homo sapiens
4 MED17  
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
5 MED7  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 TRRAP 8295
Co-purification Homo sapiens
7 TOP2B 7155
Affinity Capture-MS Homo sapiens
8 MED6  
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 MED29  
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 KPNB1 3837
Affinity Capture-MS Homo sapiens
11 QKI 9444
Affinity Capture-MS Homo sapiens
12 FBXL19  
Affinity Capture-MS Homo sapiens
13 MED9  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 MED13L 23389
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 C9orf78 51759
Affinity Capture-MS Homo sapiens
16 BRD2  
Affinity Capture-MS Homo sapiens
17 APP 351
Reconstituted Complex Homo sapiens
18 Med22  
Affinity Capture-MS Mus musculus
19 MED12L  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 MED13 9969
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
21 FBXW7  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
22 PPP6R1 22870
Co-fractionation Homo sapiens
23 MED27  
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 TFIP11  
Co-fractionation Homo sapiens
25 RLTPR 146206
Affinity Capture-MS Homo sapiens
26 MED22  
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 TACSTD2 4070
Affinity Capture-MS Homo sapiens
28 BRD4 23476
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 ATG16L1 55054
Affinity Capture-MS Homo sapiens
30 XRCC3  
Affinity Capture-MS Homo sapiens
31 ZWINT  
Affinity Capture-MS Homo sapiens
32 VDR  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 GSK3B 2932
Two-hybrid Homo sapiens
34 MED4 29079
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
35 MED1 5469
Co-purification Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
36 TNFSF11  
Two-hybrid Homo sapiens
37 MED20 9477
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 CTDP1 9150
Reconstituted Complex Homo sapiens
39 C12orf49  
Positive Genetic Homo sapiens
40 LDHC 3948
Affinity Capture-MS Homo sapiens
41 SMAD3 4088
Affinity Capture-Western Homo sapiens
42 MED26  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 SMAD2 4087
Affinity Capture-Western Homo sapiens
44 PML 5371
Affinity Capture-MS Homo sapiens
45 MED25  
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
46 MED31  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 ACAD11 84129
Affinity Capture-MS Homo sapiens
48 SERPINA3 12
Affinity Capture-MS Homo sapiens
49 CEBPA  
Protein-peptide Homo sapiens
50 NR1I3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 MED15  
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 POLR2B 5431
Affinity Capture-MS Homo sapiens
53 PPP4R1 9989
Affinity Capture-MS Homo sapiens
54 HNRNPD 3184
Affinity Capture-MS Homo sapiens
55 CCNC  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 EPAS1  
Affinity Capture-MS Homo sapiens
57 PPARG 5468
Reconstituted Complex Homo sapiens
58 SREBF1 6720
Co-purification Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
59 CDK19 23097
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
60 OBFC1 79991
Affinity Capture-MS Homo sapiens
61 THRA  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
62 SMAD1 4086
Affinity Capture-Western Homo sapiens
63 HNF4A  
Reconstituted Complex Homo sapiens
64 MED10  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
65 MYC  
Proximity Label-MS Homo sapiens
66 PPARA  
Reconstituted Complex Homo sapiens
67 EMC2 9694
Affinity Capture-MS Homo sapiens
68 MED23 9439
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
69 Junb  
Affinity Capture-MS Mus musculus
70 Rpl35 66489
Affinity Capture-MS Mus musculus
71 BRD3 8019
Affinity Capture-MS Homo sapiens
72 MED14  
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
73 AR 367
Two-hybrid Homo sapiens
Proximity Label-MS Homo sapiens
74 MED11  
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
75 Trappc8  
Affinity Capture-MS Mus musculus
76 MED19  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
77 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
78 ESR2  
Reconstituted Complex Homo sapiens
79 PARP1 142
Affinity Capture-Western Homo sapiens
80 Ewsr1 14030
Affinity Capture-MS Mus musculus
81 ILF3 3609
Affinity Capture-RNA Homo sapiens
82 CDK8 1024
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
83 MEN1 4221
Affinity Capture-MS Homo sapiens
84 MED8  
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
85 RPAP2  
Affinity Capture-MS Homo sapiens
86 LMO2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
87 KIFAP3 22920
Affinity Capture-MS Homo sapiens
88 MKI67  
Affinity Capture-MS Homo sapiens
89 MMGT1 93380
Affinity Capture-MS Homo sapiens
90 ESR1  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
91 MED30  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
92 MED16 10025
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
93 MED18  
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
94 MED21  
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
95 TCL1B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
96 MED12  
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
97 TRIP4 9325
Affinity Capture-MS Homo sapiens
98 RXRA 6256
Reconstituted Complex Homo sapiens
99 FBP1 2203
Affinity Capture-MS Homo sapiens
100 ZC3H13 23091
Affinity Capture-MS Homo sapiens
101 RPS6KA6 27330
Two-hybrid Homo sapiens
102 B3GNT8  
Affinity Capture-MS Homo sapiens
103 MED28  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which MED24 is involved
PathwayEvidenceSource
Adipogenesis TAS Reactome
Adipogenesis IEA Reactome
Developmental Biology TAS Reactome
Developmental Biology IEA Reactome
Disease TAS Reactome
Epigenetic regulation by WDR5-containing histone modifying complexes TAS Reactome
Epigenetic regulation by WDR5-containing histone modifying complexes IEA Reactome
Epigenetic regulation of adipogenesis genes by MLL3 and MLL4 complexes TAS Reactome
Epigenetic regulation of adipogenesis genes by MLL3 and MLL4 complexes IEA Reactome
Epigenetic regulation of gene expression TAS Reactome
Epigenetic regulation of gene expression IEA Reactome
Epigenetic regulation of gene expression by MLL3 and MLL4 complexes TAS Reactome
Epigenetic regulation of gene expression by MLL3 and MLL4 complexes IEA Reactome
Gene expression (Transcription) TAS Reactome
Gene expression (Transcription) IEA Reactome
Generic Transcription Pathway TAS Reactome
Infectious disease TAS Reactome
Metabolism TAS Reactome
Metabolism of lipids TAS Reactome
MLL4 and MLL3 complexes regulate expression of PPARG target genes in adipogenesis and hepatic steatosis TAS Reactome
MLL4 and MLL3 complexes regulate expression of PPARG target genes in adipogenesis and hepatic steatosis IEA Reactome
PPARA activates gene expression TAS Reactome
Regulation of lipid metabolism by PPARalpha TAS Reactome
Respiratory Syncytial Virus Infection Pathway TAS Reactome
RNA Polymerase II Transcription TAS Reactome
RSV-host interactions TAS Reactome
Transcriptional regulation of white adipocyte differentiation TAS Reactome
Transcriptional regulation of white adipocyte differentiation IEA Reactome
Viral Infection Pathways TAS Reactome





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