Gene description for SMARCA4
Gene name SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4
Gene symbol SMARCA4
Other names/aliases BAF190
BAF190A
BRG1
MRD16
RTPS2
SNF2
SNF2L4
SNF2LB
SWI2
hSNF2b
Species Homo sapiens
 Database cross references - SMARCA4
ExoCarta ExoCarta_6597
Entrez Gene 6597
HGNC 11100
MIM 603254
UniProt P51532  
 SMARCA4 identified in exosomes derived from the following tissue/cell type
Mesenchymal stem cells Unpublished / Not applicable
Thymus 23844026    
 Gene ontology annotations for SMARCA4
Molecular Function
    transcription corepressor activity GO:0003714 IDA
    protein binding GO:0005515 IPI
    RNA polymerase II distal enhancer sequence-specific DNA binding GO:0000980 IDA
    protein N-terminus binding GO:0047485 IPI
    Tat protein binding GO:0030957 IPI
    ATP binding GO:0005524 IEA
    RNA polymerase II core promoter proximal region sequence-specific DNA binding GO:0000978 IDA
    transcription coactivator activity GO:0003713 NAS
    RNA polymerase II transcription coactivator activity GO:0001105 IDA
    DNA polymerase binding GO:0070182 IPI
    nucleosomal DNA binding GO:0031492 IDA
    androgen receptor binding GO:0050681 IPI
    p53 binding GO:0002039 IPI
    DNA-dependent ATPase activity GO:0008094 IGI
    lysine-acetylated histone binding GO:0070577 IDA
    helicase activity GO:0004386 IEA
Biological Process
    cell morphogenesis GO:0000902 IEA
    liver development GO:0001889 IEA
    nucleosome disassembly GO:0006337 IDA
    extracellular matrix organization GO:0030198 IEA
    aortic smooth muscle cell differentiation GO:0035887 IEA
    definitive erythrocyte differentiation GO:0060318 IEA
    hindbrain development GO:0030902 IEA
    negative regulation of transcription, DNA-templated GO:0045892 IMP
    regulation of transcription from RNA polymerase II promoter GO:0006357 NAS
    blastocyst growth GO:0001832 IEA
    embryonic hindlimb morphogenesis GO:0035116 IEA
    embryonic organ morphogenesis GO:0048562 IEA
    vasculogenesis GO:0001570 IEA
    positive regulation of pri-miRNA transcription from RNA polymerase II promoter GO:1902895 IMP
    negative regulation of G1/S transition of mitotic cell cycle GO:2000134 TAS
    keratinocyte differentiation GO:0030216 IEA
    positive regulation of transcription, DNA-templated GO:0045893 IMP
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 IGI
    positive regulation by host of viral transcription GO:0043923 IMP
    glial cell fate determination GO:0007403 IEA
    heart trabecula formation GO:0060347 IEA
    negative regulation of transcription from RNA polymerase II promoter during mitosis GO:0007070 TAS
    negative regulation of cell growth GO:0030308 IMP
    neural retina development GO:0003407 IEP
    positive regulation of DNA binding GO:0043388 IEA
    chromatin remodeling GO:0006338 IDA
    forebrain development GO:0030900 IEA
    blastocyst hatching GO:0001835 IEA
    transcription, DNA-templated GO:0006351 IEA
    methylation-dependent chromatin silencing GO:0006346 IEA
    positive regulation of Wnt signaling pathway GO:0030177 IMP
    chromatin organization GO:0006325 TAS
    epidermis morphogenesis GO:0048730 IEA
    negative regulation of transcription from RNA polymerase II promoter GO:0000122 TAS
    lens fiber cell development GO:0070307 IEA
    DNA methylation on cytosine within a CG sequence GO:0010424 IEA
    ATP-dependent chromatin remodeling GO:0043044 IDA
    stem cell maintenance GO:0019827 IEA
    histone H3 acetylation GO:0043966 IEA
    negative regulation of androgen receptor signaling pathway GO:0060766 IMP
    positive regulation of sequence-specific DNA binding transcription factor activity GO:0051091 IDA
Subcellular Localization
    nucleoplasm GO:0005654 TAS
    nucleolus GO:0005730 IDA
    protein complex GO:0043234 IDA
    perichromatin fibrils GO:0005726 IEA
    SWI/SNF complex GO:0016514 IDA
    npBAF complex GO:0071564 ISS
    nucleus GO:0005634 IDA
    nuclear chromatin GO:0000790 IDA
    extracellular space GO:0005615 IDA
    membrane GO:0016020 IDA
    heterochromatin GO:0000792 IEA
    nBAF complex GO:0071565 ISS
    nuclear euchromatin GO:0005719 IEA
 Experiment description of studies that identified SMARCA4 in exosomes
1
Experiment ID 126
ISEV standards
EV Biophysical techniques
GAPDH
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
2
Experiment ID 217
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
CD81|CD9|CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for SMARCA4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GATA1  
Reconstituted Complex Homo sapiens
2 PHB 5245
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
3 VDR  
Affinity Capture-Western Homo sapiens
4 SMARCB1 6598
Co-purification Homo sapiens
Co-fractionation Homo sapiens
Co-purification Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
5 MPP6 51678
Two-hybrid Homo sapiens
6 STAT2 6773
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
7 CRSP6  
Co-purification Homo sapiens
8 ACTL6A 86
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
9 SIN3A  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
10 SMARCD1 6602
Affinity Capture-Western Homo sapiens
11 RB1 5925
Affinity Capture-Western Homo sapiens
12 SP1  
Reconstituted Complex Homo sapiens
13 SMARCC1 6599
Co-purification Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
14 CTNNB1 1499
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
15 HDAC2 3066
Co-purification Homo sapiens
16 NCOA1  
Phenotypic Enhancement Homo sapiens
17 BRCA1 672
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
18 KLF1  
Reconstituted Complex Homo sapiens
19 NR3C1 2908
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
20 CCNE1  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
21 CREBBP  
Phenotypic Enhancement Homo sapiens
22 NCOR2  
Co-fractionation Homo sapiens
23 POLR2A 5430
Co-purification Homo sapiens
Invitro Homo sapiens
Co-purification Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
24 RBBP4 5928
Co-purification Homo sapiens
25 ESR1  
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
26 ARID1B 57492
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
27 FANCA  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
28 NCOR1  
Co-purification Homo sapiens
29 MYC  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
30 ING1  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
31 HSF1  
Affinity Capture-Western Homo sapiens
Invitro Homo sapiens
32 AHR 196
Reconstituted Complex Homo sapiens
33 CBX5 23468
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
34 TP53  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
35 RELB  
Affinity Capture-MS Homo sapiens
36 STK11 6794
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
37 ACTB 60
Affinity Capture-Western Homo sapiens
38 ARID1A 8289
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
39 AR 367
Affinity Capture-Western Homo sapiens
40 SMARCE1 6605
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
41 HSP90B1 7184
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
42 SMARCC2 6601
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which SMARCA4 is involved
PathwayEvidenceSource
formation of the beta-catenin:TCF transactivating complex TAS Reactome
RMTs methylate histone arginines TAS Reactome





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