Gene description for SMAD1
Gene name SMAD family member 1
Gene symbol SMAD1
Other names/aliases BSP-1
BSP1
JV4-1
JV41
MADH1
MADR1
Species Homo sapiens
 Database cross references - SMAD1
ExoCarta ExoCarta_4086
Vesiclepedia VP_4086
Entrez Gene 4086
HGNC 6767
MIM 601595
UniProt Q15797  
 SMAD1 identified in sEVs derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
 Gene ontology annotations for SMAD1
Molecular Function
    RNA polymerase II cis-regulatory region sequence-specific DNA binding GO:0000978 IBA
    RNA polymerase II cis-regulatory region sequence-specific DNA binding GO:0000978 IDA
    DNA-binding transcription factor activity, RNA polymerase II-specific GO:0000981 IBA
    DNA-binding transcription factor activity, RNA polymerase II-specific GO:0000981 IDA
    DNA-binding transcription factor activity, RNA polymerase II-specific GO:0000981 ISA
    DNA-binding transcription activator activity, RNA polymerase II-specific GO:0001228 IDA
    DNA-binding transcription factor activity GO:0003700 IDA
    protein binding GO:0005515 IPI
    DEAD/H-box RNA helicase binding GO:0017151 IPI
    protein kinase binding GO:0019901 IPI
    ubiquitin protein ligase binding GO:0031625 IPI
    identical protein binding GO:0042802 IPI
    metal ion binding GO:0046872 IEA
    co-SMAD binding GO:0070410 IPI
    I-SMAD binding GO:0070411 IBA
    I-SMAD binding GO:0070411 IPI
    primary miRNA binding GO:0070878 IEA
Biological Process
    MAPK cascade GO:0000165 IEA
    ossification GO:0001503 IGI
    osteoblast differentiation GO:0001649 IDA
    ureteric bud development GO:0001657 IEA
    mesodermal cell fate commitment GO:0001710 IEA
    osteoblast fate commitment GO:0002051 IEA
    cardiac conduction system development GO:0003161 NAS
    DNA-templated transcription GO:0006351 IDA
    regulation of transcription by RNA polymerase II GO:0006357 IBA
    transcription by RNA polymerase II GO:0006366 IEA
    inflammatory response GO:0006954 IEA
    signal transduction GO:0007165 NAS
    transforming growth factor beta receptor signaling pathway GO:0007179 IBA
    transforming growth factor beta receptor signaling pathway GO:0007179 IDA
    transforming growth factor beta receptor signaling pathway GO:0007179 NAS
    gamete generation GO:0007276 IEA
    negative regulation of cell population proliferation GO:0008285 IEA
    anatomical structure morphogenesis GO:0009653 IBA
    embryonic pattern specification GO:0009880 ISS
    positive regulation of gene expression GO:0010628 IEA
    cell differentiation GO:0030154 IBA
    BMP signaling pathway GO:0030509 IBA
    BMP signaling pathway GO:0030509 IDA
    BMP signaling pathway GO:0030509 IMP
    BMP signaling pathway GO:0030509 ISS
    BMP signaling pathway GO:0030509 TAS
    midbrain development GO:0030901 IEA
    hindbrain development GO:0030902 IEA
    primary miRNA processing GO:0031053 TAS
    homeostatic process GO:0042592 IEA
    positive regulation of osteoblast differentiation GO:0045669 IEA
    positive regulation of transcription by RNA polymerase II GO:0045944 IDA
    positive regulation of transcription by RNA polymerase II GO:0045944 ISS
    stem cell differentiation GO:0048863 ISS
    negative regulation of muscle cell differentiation GO:0051148 IEA
    cartilage development GO:0051216 IEA
    cardiac muscle cell proliferation GO:0060038 IEA
    bone development GO:0060348 IEA
    SMAD protein signal transduction GO:0060395 IBA
    SMAD protein signal transduction GO:0060395 IDA
    SMAD protein signal transduction GO:0060395 IGI
    SMAD protein signal transduction GO:0060395 ISS
    SMAD protein signal transduction GO:0060395 NAS
    positive regulation of cartilage development GO:0061036 IEA
    cellular response to organic cyclic compound GO:0071407 IEA
    positive regulation of dendrite development GO:1900006 NAS
    positive regulation of miRNA transcription GO:1902895 IEA
    positive regulation of sprouting angiogenesis GO:1903672 IMP
Subcellular Localization
    chromatin GO:0000785 IDA
    chromatin GO:0000785 ISA
    male germ cell nucleus GO:0001673 IDA
    nucleus GO:0005634 IDA
    nucleus GO:0005634 ISS
    nucleus GO:0005634 NAS
    nucleus GO:0005634 TAS
    nuclear inner membrane GO:0005637 IDA
    nucleoplasm GO:0005654 TAS
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 ISS
    cytosol GO:0005829 TAS
    membrane GO:0016020 NAS
    protein-containing complex GO:0032991 IDA
    SMAD protein complex GO:0071141 NAS
    homomeric SMAD protein complex GO:0071142 IPI
    heteromeric SMAD protein complex GO:0071144 IBA
    heteromeric SMAD protein complex GO:0071144 IPI
 Experiment description of studies that identified SMAD1 in sEVs
1
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for SMAD1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 NEUROG1  
Phenotypic Suppression Homo sapiens
Phenotypic Enhancement Homo sapiens
Reconstituted Complex Homo sapiens
2 XPC  
Two-hybrid Homo sapiens
3 CAMSAP1 157922
Two-hybrid Homo sapiens
4 WWP1 11059
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
5 SKI 6497
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
6 PSMD11 5717
Two-hybrid Homo sapiens
7 MAST4  
Two-hybrid Homo sapiens
8 AP2A2 161
Two-hybrid Homo sapiens
9 SMARCE1 6605
Two-hybrid Homo sapiens
10 USP9X 8239
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
11 UBC 7316
Two-hybrid Homo sapiens
12 MED24 9862
Affinity Capture-Western Homo sapiens
13 HBP1  
Two-hybrid Homo sapiens
14 APP 351
Reconstituted Complex Homo sapiens
15 CREBBP  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
16 IRF2BP1 26145
Two-hybrid Homo sapiens
17 SNRNP70 6625
Two-hybrid Homo sapiens
18 ZNF423  
Affinity Capture-Western Homo sapiens
19 HOXA5  
Two-hybrid Homo sapiens
20 SF3B1 23451
Two-hybrid Homo sapiens
21 HGS 9146
Affinity Capture-Western Homo sapiens
22 KMT2D 8085
Two-hybrid Homo sapiens
23 ZNF512B  
Two-hybrid Homo sapiens
24 NKX3-2  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
25 LEF1  
Affinity Capture-Western Homo sapiens
26 STAT3 6774
Affinity Capture-Western Homo sapiens
27 TTF1  
Two-hybrid Homo sapiens
28 PPM1A 5494
Affinity Capture-Western Homo sapiens
29 TMEM192 201931
Affinity Capture-MS Homo sapiens
30 DGCR2 9993
Affinity Capture-MS Homo sapiens
31 SMAD5 4090
Two-hybrid Homo sapiens
32 OAZ3  
Two-hybrid Homo sapiens
33 SOX5 6660
Two-hybrid Homo sapiens
34 NEDD4 4734
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
35 CPNE3 8895
Affinity Capture-MS Homo sapiens
36 GSK3B 2932
Co-localization Homo sapiens
37 NOTCH2 4853
Two-hybrid Homo sapiens
38 MAPK1 5594
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
39 NEDD9 4739
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
40 INPP4A 3631
Two-hybrid Homo sapiens
41 ZDHHC3 51304
Two-hybrid Homo sapiens
42 PSMB4 5692
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
43 RFX1  
Two-hybrid Homo sapiens
44 PIAS4  
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
45 ACVR1 90
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
PCA Homo sapiens
46 ZNF76  
Two-hybrid Homo sapiens
47 ZEB2  
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
48 YAP1 10413
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Co-crystal Structure Homo sapiens
49 GLI3 2737
Co-localization Homo sapiens
50 SMAD3 4088
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
51 GMEB1  
Two-hybrid Homo sapiens
52 HMGA2 8091
Affinity Capture-Western Homo sapiens
53 BMP7 655
Phenotypic Suppression Homo sapiens
54 SMAD2 4087
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
55 ZNF251  
Two-hybrid Homo sapiens
56 SS18L1  
Two-hybrid Homo sapiens
57 MED15  
Affinity Capture-Western Homo sapiens
58 CHMP3 51652
Two-hybrid Homo sapiens
59 PAX6  
Affinity Capture-Western Homo sapiens
60 ANKRD27  
Two-hybrid Homo sapiens
61 ZSCAN4  
Two-hybrid Homo sapiens
62 CDK7 1022
Biochemical Activity Homo sapiens
63 XPO1 7514
Affinity Capture-MS Homo sapiens
64 USP15 9958
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
65 ACVRL1 94
Biochemical Activity Homo sapiens
66 MGA  
Two-hybrid Homo sapiens
67 ERBB2IP 55914
Reconstituted Complex Homo sapiens
68 FOXG1  
Reconstituted Complex Homo sapiens
69 FRZB  
Two-hybrid Homo sapiens
70 NUP214 8021
Affinity Capture-Western Homo sapiens
71 RUNX3  
Affinity Capture-Western Homo sapiens
72 AKR1B1 231
Two-hybrid Homo sapiens
73 YY1 7528
Reconstituted Complex Homo sapiens
74 KDM3B  
Affinity Capture-MS Homo sapiens
75 ICK  
Two-hybrid Homo sapiens
76 PIAS1 8554
Two-hybrid Homo sapiens
77 RUNX2  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
78 TTF2 8458
Two-hybrid Homo sapiens
79 SMAD6  
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
80 TOB1  
Affinity Capture-Western Homo sapiens
81 MECOM 2122
Reconstituted Complex Homo sapiens
82 LEMD3  
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
83 SKIL  
Affinity Capture-Western Homo sapiens
84 ZCCHC12  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
85 PIGQ  
Two-hybrid Homo sapiens
86 SMAD7  
Affinity Capture-Western Homo sapiens
87 STUB1 10273
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Co-crystal Structure Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
88 DZIP3  
Affinity Capture-MS Homo sapiens
89 DNMT3L  
Reconstituted Complex Homo sapiens
90 DVL1 1855
Reconstituted Complex Homo sapiens
91 FOXO3  
Affinity Capture-Western Homo sapiens
92 STARD13 90627
Two-hybrid Homo sapiens
93 KAT2A  
Affinity Capture-Western Homo sapiens
94 WDR77 79084
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
95 CDK8 1024
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
96 TP53 7157
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
97 GP9 2815
Affinity Capture-MS Homo sapiens
98 MKL2  
Two-hybrid Homo sapiens
99 ZNF510  
Two-hybrid Homo sapiens
100 ZEB1  
Affinity Capture-Western Homo sapiens
101 MEN1 4221
Affinity Capture-Western Homo sapiens
102 SMAD1 4086
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
103 SMURF1 57154
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-crystal Structure Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
Protein-peptide Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
104 MED6  
Affinity Capture-Western Homo sapiens
105 SMAD4  
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Luminescence Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
106 BMPR1A 657
Biochemical Activity Homo sapiens
107 HDAC1 3065
Affinity Capture-Western Homo sapiens
108 UCHL3 7347
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
109 KDM6B 23135
Affinity Capture-Western Homo sapiens
110 RUNX1  
Affinity Capture-Western Homo sapiens
111 TGIF1  
Affinity Capture-Western Homo sapiens
112 SNIP1  
Two-hybrid Homo sapiens
113 HOXC8  
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
114 CDK9 1025
Biochemical Activity Homo sapiens
115 TCF20  
Two-hybrid Homo sapiens
116 PUM1 9698
Two-hybrid Homo sapiens
117 HIPK2 28996
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
118 OAZ1 4946
Two-hybrid Homo sapiens
119 AR 367
Affinity Capture-Western Homo sapiens
120 SMURF2 64750
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
121 PSMD1 5707
Two-hybrid Homo sapiens
122 EP300 2033
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
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