Gene description for UCHL1
Gene name ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
Gene symbol UCHL1
Other names/aliases HEL-117
NDGOA
PARK5
PGP 9.5
PGP9.5
PGP95
Uch-L1
Species Homo sapiens
 Database cross references - UCHL1
ExoCarta ExoCarta_7345
Entrez Gene 7345
HGNC 12513
MIM 191342
UniProt P09936  
 UCHL1 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Prostate cancer cells 25844599    
Prostate cancer cells 25844599    
 Gene ontology annotations for UCHL1
Molecular Function
    ligase activity GO:0016874 IEA
    alpha-2A adrenergic receptor binding GO:0031694 IPI
    cysteine-type endopeptidase activity GO:0004197 IDA
    ubiquitin binding GO:0043130 IDA
    ubiquitin-specific protease activity GO:0004843 IBA
    ubiquitin protein ligase binding GO:0031625 IPI
    protein binding GO:0005515 IPI
    omega peptidase activity GO:0008242 IDA
Biological Process
    adult walking behavior GO:0007628 IEA
    axon target recognition GO:0007412 IEA
    neuromuscular process GO:0050905 IEA
    cell proliferation GO:0008283 IEA
    response to ischemia GO:0002931 IEA
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 NAS
    sensory perception of pain GO:0019233 IEA
    negative regulation of MAP kinase activity GO:0043407 IDA
    protein deubiquitination GO:0016579 IDA
    axon transport of mitochondrion GO:0019896 IEA
    eating behavior GO:0042755 IEA
    muscle fiber development GO:0048747 IEA
Subcellular Localization
    neuronal cell body GO:0043025 IEA
    cytoplasm GO:0005737 TAS
    endoplasmic reticulum membrane GO:0005789 IEA
    plasma membrane GO:0005886 IDA
    extracellular exosome GO:0070062 IDA
    axon GO:0030424 IEA
    nucleoplasm GO:0005654 IDA
    cytosol GO:0005829 IEA
 Experiment description of studies that identified UCHL1 in exosomes
1
Experiment ID 236
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 211
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
cytochrome c|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 212
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
Cytochrome C|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 275
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|RAB5A
EV Cytosolic markers
CD9|CD82|CD63|CD81
EV Membrane markers
AIF
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T.
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel sensitive
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.12-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry/Flow cytometry/Western blotting
5
Experiment ID 274
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|RAB5A
EV Cytosolic markers
CD9|CD82|CD63|CD81
EV Membrane markers
AIF
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T.
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel resistant
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Flow cytometry
Western blotting
 Protein-protein interactions for UCHL1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 COPS5 10987
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
2 RANBP9 10048
Two-hybrid Homo sapiens
3 UBE2I 7329
Two-hybrid Homo sapiens
4 CBX1 10951
Two-hybrid Homo sapiens
5 CDKN1B 1027
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
6 UBC 7316
Two-hybrid Homo sapiens
7 NEDD8 4738
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which UCHL1 is involved
No pathways found





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