Experiment description of studies that identified RPSAP15 in sEVs

1 Experiment ID 21 MISEV standards ✔ EM|IEM Biophysical techniques ✔ Alix|TSG101|HSP70|CD63 Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry    PubMed ID 19837982     Organism Homo sapiens Experiment description Proteomic and bioinformatic analysis of immunoaffinity-purified exosomes derived from the human colon tumor cell line LIM1215. Authors "Suresh Mathivanan, Justin W.E. Lim, Bow J. Tauro, Hong Ji, Robert L. Moritz and Richard J. Simpson" Journal name MCP Publication year 2009 Sample Colorectal cancer cells Sample name LIM1215 Isolation/purification methods Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.10-1.12 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry [Orbitrap] Western blotting

2 Experiment ID 126 MISEV standards ✘ Biophysical techniques ✔ GAPDH Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry [LTQ-FT Ultra] PubMed ID Unpublished / Not applicable Organism Homo sapiens Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy? Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim" Journal name Mesenchymal Stem Cell Therapy Publication year 2011 Sample Mesenchymal stem cells Sample name huES9.E1 Isolation/purification methods HPLC Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Antobody array Mass spectrometry

3 Experiment ID 219 MISEV standards ✔ EM Biophysical techniques ✔ CD63|CD9 Enriched markers ✔ HSP90B1 Negative markers ✔ qNano Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25857718     Organism Homo sapiens Experiment description Proteomic analysis of exosomes from nasopharyngeal carcinoma cell identifies intercellular transfer of angiogenic proteins Authors "Chan YK, Zhang H, Liu P, Tsao GS, Li Lung M, Mak NK, Ngok-Shun Wong R, Ying-Kit Yue P" Journal name Int J Cancer Publication year 2015 Sample Nasopharyngeal carcinoma cells Sample name C666-1 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.17-1.19 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

4 Experiment ID 220 MISEV standards ✔ EM Biophysical techniques ✔ CD63|CD9 Enriched markers ✔ HSP90B1 Negative markers ✔ qNano Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25857718     Organism Homo sapiens Experiment description Proteomic analysis of exosomes from nasopharyngeal carcinoma cell identifies intercellular transfer of angiogenic proteins Authors "Chan YK, Zhang H, Liu P, Tsao GS, Li Lung M, Mak NK, Ngok-Shun Wong R, Ying-Kit Yue P" Journal name Int J Cancer Publication year 2015 Sample Nasopharyngeal carcinoma cells Sample name NP69 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.17-1.19 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

5 Experiment ID 221 MISEV standards ✔ EM Biophysical techniques ✔ CD63|CD9 Enriched markers ✔ HSP90B1 Negative markers ✔ qNano Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25857718     Organism Homo sapiens Experiment description Proteomic analysis of exosomes from nasopharyngeal carcinoma cell identifies intercellular transfer of angiogenic proteins Authors "Chan YK, Zhang H, Liu P, Tsao GS, Li Lung M, Mak NK, Ngok-Shun Wong R, Ying-Kit Yue P" Journal name Int J Cancer Publication year 2015 Sample Nasopharyngeal carcinoma cells Sample name NP460 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.17-1.19 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

Protein-protein interactions for RPSAP15

Protein Interactor ExoCarta ID Identification method PubMed Species 1 COPS5 10987 Affinity Capture-MS Homo sapiens 2 CRBN   Affinity Capture-MS Homo sapiens 3 HNF1A   Affinity Capture-MS Homo sapiens 4 CUL1 8454 Affinity Capture-MS Homo sapiens 5 CUL3 8452 Affinity Capture-MS Homo sapiens 6 CAND1 55832 Affinity Capture-MS Homo sapiens 7 SMAD3 4088 Affinity Capture-MS Homo sapiens View the network

Pathways in which RPSAP15 is involved

No pathways found