Gene description for HIPK2
Gene name homeodomain interacting protein kinase 2
Gene symbol HIPK2
Other names/aliases PRO0593
Species Homo sapiens
 Database cross references - HIPK2
ExoCarta ExoCarta_28996
Entrez Gene 28996
HGNC 14402
MIM 606868
UniProt Q9H2X6  
 HIPK2 identified in exosomes derived from the following tissue/cell type
Endothelial cells 26027894    
 Gene ontology annotations for HIPK2
Molecular Function
    protein binding GO:0005515 IPI
    RNA polymerase II transcription coactivator activity GO:0001105 ISS
    protein serine/threonine kinase activity GO:0004674 ISS
    RNA polymerase II activating transcription factor binding GO:0001102 ISS
    virion binding GO:0046790 IPI
    transcription corepressor activity GO:0003714 IDA
    ATP binding GO:0005524 IEA
    protein kinase activity GO:0004672 IDA
    SMAD binding GO:0046332 IPI
Biological Process
    intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator GO:0042771 TAS
    positive regulation of protein binding GO:0032092 ISS
    smoothened signaling pathway GO:0007224 IEA
    cellular response to hypoxia GO:0071456 TAS
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 ISS
    positive regulation of angiogenesis GO:0045766 IDA
    positive regulation of DNA binding GO:0043388 IEA
    eye development GO:0001654 ISS
    positive regulation of cell proliferation GO:0008284 IEA
    negative regulation of transcription from RNA polymerase II promoter GO:0000122 IEA
    positive regulation of transforming growth factor beta receptor signaling pathway GO:0030511 IMP
    transforming growth factor beta receptor signaling pathway GO:0007179 IEA
    adult walking behavior GO:0007628 IEA
    iris morphogenesis GO:0061072 IEA
    positive regulation of transcription, DNA-templated GO:0045893 IMP
    DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator GO:0006978 IDA
    positive regulation of JNK cascade GO:0046330 IMP
    neuron differentiation GO:0030182 IEA
    anterior/posterior pattern specification GO:0009952 IEA
    negative regulation of BMP signaling pathway GO:0030514 IMP
    erythrocyte differentiation GO:0030218 ISS
    peptidyl-threonine phosphorylation GO:0018107 ISS
    embryonic retina morphogenesis in camera-type eye GO:0060059 IEA
    modulation by virus of host morphology or physiology GO:0019048 NAS
    peptidyl-serine phosphorylation GO:0018105 ISS
    regulation of cell cycle GO:0051726 TAS
    transcription, DNA-templated GO:0006351 IEA
    voluntary musculoskeletal movement GO:0050882 IEA
    protein phosphorylation GO:0006468 IDA
    SMAD protein signal transduction GO:0060395 IDA
    embryonic camera-type eye morphogenesis GO:0048596 IEA
    positive regulation of sequence-specific DNA binding transcription factor activity GO:0051091 ISS
    lens induction in camera-type eye GO:0060235 IEA
    negative regulation of neuron apoptotic process GO:0043524 IEA
    retina layer formation GO:0010842 IEA
    PML body organization GO:0030578 TAS
    intrinsic apoptotic signaling pathway GO:0097193 NAS
Subcellular Localization
    nucleus GO:0005634 IDA
    PML body GO:0016605 IDA
    nuclear body GO:0016604 TAS
    RNA polymerase II transcription factor complex GO:0090575 ISS
    cytoplasm GO:0005737 ISS
 Experiment description of studies that identified HIPK2 in exosomes
1
Experiment ID 226
ISEV standards
EM
EV Biophysical techniques
GAPDH
EV Cytosolic markers
CD9|FLOT1
EV Membrane markers
LMNA|H2AFX|ATP5A1|TOMM20
EV Negative markers
EV Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting
Authors Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
 Protein-protein interactions for HIPK2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Ube2i  
Reconstituted Complex Mus musculus
Two-hybrid Mus musculus
2 TP53INP1  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
3 SMAD2 4087
Reconstituted Complex Homo sapiens
4 DAXX  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
5 UBE2I 7329
Two-hybrid Homo sapiens
6 SMAD1  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
7 TRADD 8717
Invitro Homo sapiens
Two-hybrid Homo sapiens
8 TP73L  
Invitro Homo sapiens
Two-hybrid Homo sapiens
9 HMGA1 3159
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
10 SMAD3  
Reconstituted Complex Homo sapiens
11 SP100  
Affinity Capture-Western Homo sapiens
12 TP73  
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
13 CREBBP  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
14 NKX3-1  
Invitro Homo sapiens
Invivo Homo sapiens
15 RANBP9 10048
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
16 TITF1  
Two-hybrid Homo sapiens
17 TP53  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
18 SUMO1 7341
Two-hybrid Homo sapiens
19 SKI 6497
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which HIPK2 is involved
PathwayEvidenceSource
YAP1- and WWTR1 (TAZ)-stimulated gene expression TAS Reactome





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