Gene description for LBP
Gene name lipopolysaccharide binding protein
Gene symbol LBP
Other names/aliases BPIFD2
Species Homo sapiens
 Database cross references - LBP
ExoCarta ExoCarta_3929
Vesiclepedia VP_3929
Entrez Gene 3929
HGNC 6517
MIM 151990
UniProt P18428  
 LBP identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Urine 19056867    
 Gene ontology annotations for LBP
Molecular Function
    lipopolysaccharide binding GO:0001530 IBA
    lipopolysaccharide binding GO:0001530 IDA
    lipopolysaccharide binding GO:0001530 IMP
    lipopolysaccharide binding GO:0001530 ISS
    signaling receptor binding GO:0005102 ISS
    protein binding GO:0005515 IPI
    coreceptor activity GO:0015026 IPI
    lipoteichoic acid binding GO:0070891 IDA
    lipopeptide binding GO:0071723 IDA
Biological Process
    leukocyte chemotaxis involved in inflammatory response GO:0002232 IEA
    macrophage activation involved in immune response GO:0002281 IBA
    macrophage activation involved in immune response GO:0002281 IMP
    macrophage activation involved in immune response GO:0002281 ISS
    cell surface pattern recognition receptor signaling pathway GO:0002752 IDA
    acute-phase response GO:0006953 IBA
    acute-phase response GO:0006953 IEP
    acute-phase response GO:0006953 ISS
    cellular defense response GO:0006968 ISS
    opsonization GO:0008228 IC
    opsonization GO:0008228 ISS
    lipopolysaccharide transport GO:0015920 IDA
    neutrophil chemotaxis GO:0030593 IEA
    lipopolysaccharide-mediated signaling pathway GO:0031663 IBA
    lipopolysaccharide-mediated signaling pathway GO:0031663 IDA
    detection of molecule of bacterial origin GO:0032490 IDA
    response to lipopolysaccharide GO:0032496 IDA
    negative regulation of tumor necrosis factor production GO:0032720 IDA
    positive regulation of chemokine production GO:0032722 IEA
    positive regulation of interleukin-6 production GO:0032755 IDA
    positive regulation of interleukin-8 production GO:0032757 IDA
    positive regulation of tumor necrosis factor production GO:0032760 IDA
    positive regulation of tumor necrosis factor production GO:0032760 IDA
    positive regulation of tumor necrosis factor production GO:0032760 IMP
    positive regulation of tumor necrosis factor production GO:0032760 ISS
    positive regulation of toll-like receptor 4 signaling pathway GO:0034145 IDA
    positive regulation of macrophage activation GO:0043032 IBA
    positive regulation of macrophage activation GO:0043032 IDA
    innate immune response GO:0045087 IBA
    innate immune response GO:0045087 IC
    innate immune response GO:0045087 ISS
    defense response to Gram-negative bacterium GO:0050829 IBA
    defense response to Gram-negative bacterium GO:0050829 IDA
    defense response to Gram-negative bacterium GO:0050829 ISS
    defense response to Gram-positive bacterium GO:0050830 IBA
    defense response to Gram-positive bacterium GO:0050830 IDA
    positive regulation of respiratory burst involved in inflammatory response GO:0060265 ISS
    cellular response to lipopolysaccharide GO:0071222 IDA
    cellular response to lipopolysaccharide GO:0071222 IMP
    cellular response to lipoteichoic acid GO:0071223 IDA
    positive regulation of neutrophil chemotaxis GO:0090023 IEA
Subcellular Localization
    extracellular region GO:0005576 TAS
    extracellular space GO:0005615 IBA
    extracellular space GO:0005615 IDA
    extracellular space GO:0005615 ISS
    cell surface GO:0009986 IDA
    membrane GO:0016020 IEA
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified LBP in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for LBP
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PYCRL 65263
Two-hybrid Homo sapiens
2 CD14 929
Reconstituted Complex Homo sapiens
Co-purification Homo sapiens
3 BAG6 7917
Two-hybrid Homo sapiens
4 SMAD3 4088
Two-hybrid Homo sapiens
5 SETD1A 9739
Two-hybrid Homo sapiens
6 PSMA3 5684
Two-hybrid Homo sapiens
7 TSC22D4 81628
Two-hybrid Homo sapiens
8 CBFA2T2  
Two-hybrid Homo sapiens
9 C4BPA 722
Two-hybrid Homo sapiens
10 HSPB1 3315
Two-hybrid Homo sapiens
11 TRA2A 29896
Two-hybrid Homo sapiens
12 BST1 683
Two-hybrid Homo sapiens
13 CFHR1  
Affinity Capture-Western Homo sapiens
14 FTH1 2495
Two-hybrid Homo sapiens
15 APOA1 335
Affinity Capture-Western Homo sapiens
16 PRDX4 10549
Two-hybrid Homo sapiens
17 SOX2  
Affinity Capture-MS Homo sapiens
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