Gene description for GOLGA2
Gene name golgin A2
Gene symbol GOLGA2
Other names/aliases GM130
Species Homo sapiens
 Database cross references - GOLGA2
ExoCarta ExoCarta_2801
Entrez Gene 2801
HGNC 4425
MIM 602580
UniProt Q08379  
 GOLGA2 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Thymus 23844026    
 Gene ontology annotations for GOLGA2
Molecular Function
    syntaxin binding GO:0019905 IPI
    protein binding GO:0005515 IPI
    protein kinase binding GO:0019901 IPI
Biological Process
    negative regulation of protein binding GO:0032091 IDA
    mitotic cell cycle GO:0000278 TAS
Subcellular Localization
    Golgi membrane GO:0000139 TAS
    Golgi cis cisterna GO:0000137 IDA
    Golgi apparatus GO:0005794 IDA
    Golgi cisterna membrane GO:0032580 IEA
    cis-Golgi network GO:0005801 IDA
    nucleus GO:0005634 IDA
    ER to Golgi transport vesicle GO:0030134 IMP
 Experiment description of studies that identified GOLGA2 in exosomes
1
Experiment ID 207
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 208
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 209
ISEV standards
✘ EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 217
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
CD81|CD9|CD63
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for GOLGA2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RAB2A 5862
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
2 RAB33B 83452
Invitro Homo sapiens
3 TRIM29  
Two-hybrid Homo sapiens
4 TMED2 10959
Affinity Capture-Western Homo sapiens
5 TUBGCP4 27229
Two-hybrid Homo sapiens
6 USO1 8615
Reconstituted Complex Homo sapiens
7 ABLIM1  
Two-hybrid Homo sapiens
8 ZNF594  
Two-hybrid Homo sapiens
9 POM121  
Two-hybrid Homo sapiens
10 RAB1A 5861
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
11 ZNF250  
Two-hybrid Homo sapiens
12 GORASP1  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
13 GORASP2 26003
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
14 RAB1B 81876
Invitro Homo sapiens
Two-hybrid Homo sapiens
15 GPS2  
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which GOLGA2 is involved
PathwayEvidenceSource
Golgi Cisternae Pericentriolar Stack Reorganization TAS Reactome





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