Gene description for NCOA2
Gene name nuclear receptor coactivator 2
Gene symbol NCOA2
Other names/aliases GRIP1
KAT13C
NCoA-2
SRC2
TIF2
bHLHe75
Species Homo sapiens
 Database cross references - NCOA2
ExoCarta ExoCarta_10499
Entrez Gene 10499
HGNC 7669
MIM 601993
UniProt Q15596  
 NCOA2 identified in exosomes derived from the following tissue/cell type
Hepatocytes 26054723    
 Gene ontology annotations for NCOA2
Molecular Function
    nuclear hormone receptor binding GO:0035257 IPI
    thyroid hormone receptor coactivator activity GO:0030375 IEA
    protein dimerization activity GO:0046983 IEA
    transcription coactivator activity GO:0003713 NAS
    transcription factor binding GO:0008134 IEA
    ligand-dependent nuclear receptor binding GO:0016922 IPI
    ligand-dependent nuclear receptor transcription coactivator activity GO:0030374 IDA
    histone acetyltransferase activity GO:0004402 IEA
    chromatin binding GO:0003682 IEA
    protein binding GO:0005515 IPI
    signal transducer activity GO:0004871 IEA
Biological Process
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 IBA
    regulation of glucose metabolic process GO:0010906 IEA
    cellular lipid metabolic process GO:0044255 TAS
    chromatin organization GO:0006325 TAS
    cellular response to hormone stimulus GO:0032870 IBA
    histone acetylation GO:0016573 IEA
    small molecule metabolic process GO:0044281 TAS
    small GTPase mediated signal transduction GO:0007264 TAS
    circadian regulation of gene expression GO:0032922 ISS
    negative regulation of transcription from RNA polymerase II promoter GO:0000122 IEA
    regulation of transcription, DNA-templated GO:0006355 NAS
    bile acid and bile salt transport GO:0015721 TAS
    positive regulation of receptor activity GO:2000273 IEA
    bile acid metabolic process GO:0008206 TAS
    transcription, DNA-templated GO:0006351 IEA
Subcellular Localization
    nucleoplasm GO:0005654 TAS
    cytoplasm GO:0005737 IEA
    nucleus GO:0005634 IBA
 Experiment description of studies that identified NCOA2 in exosomes
1
Experiment ID 237
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for NCOA2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Smarce1  
Reconstituted Complex Mus musculus
2 PPARG 5468
Invitro Homo sapiens
Two-hybrid Homo sapiens
3 RXRA  
Two-hybrid Homo sapiens
4 ESR1  
Affinity Capture-Western Homo sapiens
Co-crystal Structure Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
5 HNF4A  
Two-hybrid Homo sapiens
6 AR 367
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Protein-peptide Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
7 TITF1  
Two-hybrid Homo sapiens
8 VDR  
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
9 PPFIA1 8500
Affinity Capture-Western Homo sapiens
10 ESRRB  
Reconstituted Complex Homo sapiens
11 MAGEA11  
Affinity Capture-Western Homo sapiens
12 NR3C1 2908
Reconstituted Complex Homo sapiens
Co-crystal Structure Homo sapiens
13 ARNT 405
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
14 PRMT1 3276
Reconstituted Complex Homo sapiens
15 PPFIA4  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
16 AHR 196
Affinity Capture-Western Homo sapiens
17 THRB 7068
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
18 NCOA1  
Invitro Homo sapiens
19 BRCA1 672
Protein-peptide Homo sapiens
Reconstituted Complex Homo sapiens
20 ANKRD11 29123
Reconstituted Complex Homo sapiens
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