Gene description for NCF2
Gene name neutrophil cytosolic factor 2
Gene symbol NCF2
Other names/aliases NCF-2
NOXA2
P67-PHOX
P67PHOX
Species Homo sapiens
 Database cross references - NCF2
ExoCarta ExoCarta_4688
Vesiclepedia VP_4688
Entrez Gene 4688
HGNC 7661
MIM 608515
UniProt P19878  
 NCF2 identified in exosomes derived from the following tissue/cell type
Thymus 23844026    
 Gene ontology annotations for NCF2
Molecular Function
    protein binding GO:0005515 IPI
    electron transfer activity GO:0009055 TAS
    superoxide-generating NAD(P)H oxidase activity GO:0016175 IEA
    superoxide-generating NADPH oxidase activator activity GO:0016176 IBA
    superoxide-generating NADPH oxidase activator activity GO:0016176 IMP
    small GTPase binding GO:0031267 IEA
Biological Process
    superoxide metabolic process GO:0006801 TAS
    phagocytosis GO:0006909 IEA
    cellular defense response GO:0006968 TAS
    superoxide anion generation GO:0042554 IBA
    superoxide anion generation GO:0042554 IMP
    superoxide anion generation GO:0042554 TAS
    innate immune response GO:0045087 TAS
    respiratory burst GO:0045730 NAS
    respiratory burst GO:0045730 TAS
Subcellular Localization
    acrosomal vesicle GO:0001669 IEA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 NAS
    membrane GO:0016020 IDA
    phagolysosome GO:0032010 TAS
    NADPH oxidase complex GO:0043020 IDA
    NADPH oxidase complex GO:0043020 NAS
    NADPH oxidase complex GO:0043020 TAS
 Experiment description of studies that identified NCF2 in exosomes
1
Experiment ID 217
MISEV standards
EM
Biophysical techniques
TSG101|CD81|CD9|CD63
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for NCF2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 BPGM 669
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 TRAF4 9618
Affinity Capture-Western Homo sapiens
3 PLA2G4A 5321
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
4 TBC1D4  
Affinity Capture-MS Homo sapiens
5 TARDBP 23435
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 CAMSAP3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 NCF4 4689
Two-hybrid Homo sapiens
Co-crystal Structure Homo sapiens
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
8 USP47 55031
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 GNB1 2782
Affinity Capture-Western Homo sapiens
10 KIFAP3 22920
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
11 NCF1  
Reconstituted Complex Homo sapiens
Far Western Homo sapiens
Reconstituted Complex Homo sapiens
Protein-peptide Homo sapiens
Co-crystal Structure Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
12 GNAI1 2770
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
13 CDC42 998
Reconstituted Complex Homo sapiens
14 GOLGA2 2801
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
15 KLK10 5655
Affinity Capture-MS Homo sapiens
16 CYBA 1535
Reconstituted Complex Homo sapiens
Protein-peptide Homo sapiens
17 PRDX6 9588
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
18 S100A8 6279
Reconstituted Complex Homo sapiens
19 SYTL1 84958
Reconstituted Complex Homo sapiens
20 S100A9 6280
Reconstituted Complex Homo sapiens
21 GNAI2 2771
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
22 RAC1 5879
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Co-crystal Structure Homo sapiens
Affinity Capture-Western Homo sapiens
23 RAC2 5880
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
24 PRKDC 5591
Biochemical Activity Homo sapiens
25 GNAI3 2773
Affinity Capture-Western Homo sapiens
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