Gene description for NCF2
Gene name neutrophil cytosolic factor 2
Gene symbol NCF2
Other names/aliases NCF-2
NOXA2
P67-PHOX
P67PHOX
Species Homo sapiens
 Database cross references - NCF2
ExoCarta ExoCarta_4688
Entrez Gene 4688
HGNC 7661
MIM 608515
UniProt P19878  
 NCF2 identified in exosomes derived from the following tissue/cell type
Thymus 23844026    
 Gene ontology annotations for NCF2
Molecular Function
    Rac GTPase binding GO:0048365 IEA
    protein C-terminus binding GO:0008022 IPI
    protein binding GO:0005515 IPI
    electron carrier activity GO:0009055 TAS
Biological Process
    response to lipopolysaccharide GO:0032496 IEA
    superoxide anion generation GO:0042554 TAS
    response to hyperoxia GO:0055093 IEA
    antigen processing and presentation of exogenous peptide antigen via MHC class I GO:0042590 TAS
    interaction with host GO:0051701 TAS
    antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent GO:0002479 TAS
    phagosome maturation GO:0090382 TAS
    response to progesterone GO:0032570 IEA
    cellular response to hormone stimulus GO:0032870 IEA
    positive regulation of neuron apoptotic process GO:0043525 IEA
    innate immune response GO:0045087 TAS
    respiratory burst GO:0045730 TAS
    cellular defense response GO:0006968 TAS
    vascular endothelial growth factor receptor signaling pathway GO:0048010 TAS
    superoxide metabolic process GO:0006801 TAS
    antigen processing and presentation of peptide antigen via MHC class I GO:0002474 TAS
    response to glucose GO:0009749 IEA
    response to drug GO:0042493 IEA
    positive regulation of blood pressure GO:0045777 IEA
    aging GO:0007568 IEA
    small GTPase mediated signal transduction GO:0007264 TAS
    response to activity GO:0014823 IEA
    response to laminar fluid shear stress GO:0034616 IEA
Subcellular Localization
    nucleolus GO:0005730 IDA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 TAS
    acrosomal vesicle GO:0001669 IEA
    phagolysosome GO:0032010 TAS
    NADPH oxidase complex GO:0043020 TAS
 Experiment description of studies that identified NCF2 in exosomes
1
Experiment ID 217
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
CD81|CD9|CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for NCF2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CDC42 998
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
2 SYTL1 84958
Reconstituted Complex Homo sapiens
3 ACTB 60
Far Western Homo sapiens
4 NCF4 4689
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
5 CYBB 1536
Affinity Capture-MS Homo sapiens
6 RAC1 5879
Invitro Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
7 RAC2 5880
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
8 CYBA 1535
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Protein-peptide Homo sapiens
9 PRDX6 9588
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
10 NCF1  
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Far Western Homo sapiens
Reconstituted Complex Homo sapiens
Protein-peptide Homo sapiens
Reconstituted Complex Homo sapiens
11 PRKDC 5591
Biochemical Activity Homo sapiens
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