Gene description for IPO8
Gene name importin 8
Gene symbol IPO8
Other names/aliases RANBP8
Species Homo sapiens
 Database cross references - IPO8
ExoCarta ExoCarta_10526
Vesiclepedia VP_10526
Entrez Gene 10526
HGNC 9853
MIM 605600
UniProt O15397  
 IPO8 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Mesenchymal stem cells 36408942    
Neuroblastoma cells 25944692    
Thymus 23844026    
 Gene ontology annotations for IPO8
Molecular Function
    protein binding GO:0005515 IPI
    small GTPase binding GO:0031267 IEA
Biological Process
    protein import into nucleus GO:0006606 IBA
    signal transduction GO:0007165 TAS
Subcellular Localization
    nuclear envelope GO:0005635 IBA
    nucleoplasm GO:0005654 TAS
    cytosol GO:0005829 IBA
    cytosol GO:0005829 TAS
 Experiment description of studies that identified IPO8 in exosomes
1
Experiment ID 207
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 208
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 209
MISEV standards
✘ Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
5
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
8
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
9
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for IPO8
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 ACAD8 27034
Affinity Capture-MS Homo sapiens
3 SLIRP 81892
Proximity Label-MS Homo sapiens
4 KPNA3 3839
Co-fractionation Homo sapiens
5 CDX2  
Affinity Capture-MS Homo sapiens
6 RIN3  
Affinity Capture-MS Homo sapiens
7 TNPO1 3842
Co-fractionation Homo sapiens
8 YWHAE 7531
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 KPNB1 3837
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
10 CHCHD1  
Proximity Label-MS Homo sapiens
11 ZNF354C  
Affinity Capture-MS Homo sapiens
12 FFAR1  
Affinity Capture-MS Homo sapiens
13 PNKD  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 LRPPRC 10128
Reconstituted Complex Homo sapiens
15 KIAA1429 25962
Affinity Capture-MS Homo sapiens
16 CAPZB 832
Affinity Capture-MS Homo sapiens
17 TRIM28 10155
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 PCNT  
Proximity Label-MS Homo sapiens
19 ZBTB10 65986
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 ZNF74  
Affinity Capture-MS Homo sapiens
21 WT1  
Two-hybrid Homo sapiens
22 ZNF524  
Affinity Capture-MS Homo sapiens
23 FAM174A 345757
Affinity Capture-MS Homo sapiens
24 AGO3  
Affinity Capture-MS Homo sapiens
Co-localization Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
25 ZSCAN21  
Affinity Capture-MS Homo sapiens
26 POU5F1  
Affinity Capture-MS Homo sapiens
27 ZNF460  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 MAD2L2 10459
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 ZSCAN31  
Affinity Capture-MS Homo sapiens
30 ZNF7  
Affinity Capture-MS Homo sapiens
31 SLX4  
Affinity Capture-MS Homo sapiens
32 BCOR  
Affinity Capture-MS Homo sapiens
33 F2RL1  
Affinity Capture-MS Homo sapiens
34 SNX27 81609
Affinity Capture-MS Homo sapiens
35 TIMM50 92609
Affinity Capture-MS Homo sapiens
36 CBLB 868
Affinity Capture-MS Homo sapiens
37 GPR17 2840
Affinity Capture-MS Homo sapiens
38 AGO2 27161
Co-localization Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
39 FSCN1 6624
Affinity Capture-MS Homo sapiens
40 BSG 682
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 KRT8 3856
Proximity Label-MS Homo sapiens
42 ZFP1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 GALNT16 57452
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 IPO9 55705
Co-fractionation Homo sapiens
45 PARK2  
Affinity Capture-MS Homo sapiens
46 STOM 2040
Affinity Capture-MS Homo sapiens
47 P2RY8  
Affinity Capture-MS Homo sapiens
48 ZFP2  
Two-hybrid Homo sapiens
49 TNFSF8  
Affinity Capture-MS Homo sapiens
50 DNAJB7  
Proximity Label-MS Homo sapiens
51 GOLGA2 2801
Proximity Label-MS Homo sapiens
52 COMTD1 118881
Affinity Capture-MS Homo sapiens
53 DIRAS3  
Proximity Label-MS Homo sapiens
54 RAN 5901
Reconstituted Complex Homo sapiens
55 S1PR1 1901
Affinity Capture-MS Homo sapiens
56 CBX5 23468
Affinity Capture-MS Homo sapiens
57 IFI16 3428
Affinity Capture-MS Homo sapiens
58 GPR182  
Affinity Capture-MS Homo sapiens
59 DCTN1 1639
Proximity Label-MS Homo sapiens
60 RPA4  
Proximity Label-MS Homo sapiens
61 ZNF784  
Affinity Capture-MS Homo sapiens
62 STIL  
Proximity Label-MS Homo sapiens
63 HECTD1 25831
Affinity Capture-MS Homo sapiens
64 RPLP0 6175
Co-fractionation Homo sapiens
65 PPM1G 5496
Co-fractionation Homo sapiens
66 CEBPA  
Proximity Label-MS Homo sapiens
67 BIN1 274
Affinity Capture-MS Homo sapiens
68 CYLD  
Affinity Capture-MS Homo sapiens
69 ZNF773  
Affinity Capture-MS Homo sapiens
70 MLNR  
Affinity Capture-MS Homo sapiens
71 ATXN1 6310
Two-hybrid Homo sapiens
72 CD40 958
Affinity Capture-MS Homo sapiens
73 MGARP  
Affinity Capture-MS Homo sapiens
74 NTRK1 4914
Affinity Capture-MS Homo sapiens
75 Trim28 21849
Affinity Capture-MS Mus musculus
76 APLNR  
Affinity Capture-MS Homo sapiens
77 MAP7 9053
Affinity Capture-MS Homo sapiens
78 SIGLECL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
79 BTNL9  
Affinity Capture-MS Homo sapiens
80 FPR1  
Affinity Capture-MS Homo sapiens
81 DNAJC2 27000
Proximity Label-MS Homo sapiens
82 NPTN 27020
Affinity Capture-MS Homo sapiens
83 P2RY2 5029
Affinity Capture-MS Homo sapiens
84 CUL3 8452
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
85 EGFR 1956
Affinity Capture-MS Homo sapiens
86 EIF4E 1977
Reconstituted Complex Homo sapiens
Co-crystal Structure Homo sapiens
Reconstituted Complex Homo sapiens
87 OSBPL5 114879
Affinity Capture-MS Homo sapiens
88 WEE1 7465
Affinity Capture-MS Homo sapiens
89 NXF1 10482
Affinity Capture-RNA Homo sapiens
90 AKR1C2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
91 RPA3 6119
Proximity Label-MS Homo sapiens
92 AGO4  
Affinity Capture-MS Homo sapiens
Co-localization Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
93 ZSCAN5A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
94 MYO6 4646
Proximity Label-MS Homo sapiens
95 FBL 2091
Proximity Label-MS Homo sapiens
96 HIST1H2BA 255626
Cross-Linking-MS (XL-MS) Homo sapiens
97 GPR45  
Affinity Capture-MS Homo sapiens
98 HP1BP3 50809
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
99 BICD1 636
Proximity Label-MS Homo sapiens
100 RPL23A 6147
Affinity Capture-Western Homo sapiens
101 DDRGK1 65992
Affinity Capture-MS Homo sapiens
102 RANBP1 5902
Affinity Capture-MS Homo sapiens
103 TNFSF13B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
104 NANOG  
Affinity Capture-MS Homo sapiens
105 ZNF696  
Affinity Capture-MS Homo sapiens
106 ZNF17  
Affinity Capture-MS Homo sapiens
107 AVPR2  
Affinity Capture-MS Homo sapiens
108 ZNF287  
Affinity Capture-MS Homo sapiens
109 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
110 ZSCAN23  
Affinity Capture-MS Homo sapiens
111 AGO1  
Co-localization Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
112 MFSD4  
Affinity Capture-MS Homo sapiens
113 ZEB1  
Affinity Capture-MS Homo sapiens
114 RAD51D  
Affinity Capture-MS Homo sapiens
115 TPTE  
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
116 OPALIN  
Affinity Capture-MS Homo sapiens
117 SRP19 6728
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
118 ZNF774  
Two-hybrid Homo sapiens
119 EFNB1 1947
Affinity Capture-MS Homo sapiens
120 COX8A  
Proximity Label-MS Homo sapiens
121 HIST1H2BD 3017
Cross-Linking-MS (XL-MS) Homo sapiens
122 ZNF764 92595
Affinity Capture-MS Homo sapiens
123 PTGER3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
124 HTR6  
Affinity Capture-MS Homo sapiens
125 SEC62 7095
Affinity Capture-MS Homo sapiens
126 ZNF629 23361
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
127 TPST2 8459
Affinity Capture-MS Homo sapiens
128 SERBP1 26135
Affinity Capture-MS Homo sapiens
129 KIR2DL4  
Affinity Capture-MS Homo sapiens
130 ARHGAP23 57636
Affinity Capture-MS Homo sapiens
131 ADGRE5 976
Affinity Capture-MS Homo sapiens
132 ZNF331  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
133 ZNF382  
Affinity Capture-MS Homo sapiens
134 PRMT1 3276
Affinity Capture-MS Homo sapiens
135 REPIN1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
136 ZSCAN25  
Affinity Capture-MS Homo sapiens
137 S1PR4  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which IPO8 is involved
PathwayEvidenceSource
Gene expression (Transcription) TAS Reactome
Gene Silencing by RNA TAS Reactome
Transcriptional regulation by small RNAs TAS Reactome





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