Gene description for EIF3G
Gene name eukaryotic translation initiation factor 3, subunit G
Gene symbol EIF3G
Other names/aliases EIF3-P42
EIF3S4
eIF3-delta
eIF3-p44
Species Homo sapiens
 Database cross references - EIF3G
ExoCarta ExoCarta_8666
Entrez Gene 8666
HGNC 3274
MIM 603913
UniProt O75821  
 EIF3G identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for EIF3G
Molecular Function
    poly(A) RNA binding GO:0044822 IDA
    nucleotide binding GO:0000166 IEA
    protein binding GO:0005515 IPI
    translation initiation factor activity GO:0003743 IDA
Biological Process
    cellular protein metabolic process GO:0044267 TAS
    regulation of translational initiation GO:0006446 IEA
    formation of translation preinitiation complex GO:0001731 IEA
    gene expression GO:0010467 TAS
    translational initiation GO:0006413 TAS
    translation GO:0006412 TAS
Subcellular Localization
    cytosol GO:0005829 TAS
    cytoplasm GO:0005737 IDA
    eukaryotic 48S preinitiation complex GO:0033290 IEA
    eukaryotic translation initiation factor 3 complex GO:0005852 IDA
    nucleus GO:0005634 IDA
    eukaryotic 43S preinitiation complex GO:0016282 IEA
    perinuclear region of cytoplasm GO:0048471 IEA
 Experiment description of studies that identified EIF3G in exosomes
1
Experiment ID 235
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for EIF3G
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 EIF3A 8661
Co-purification Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
2 MPHOSPH6  
Affinity Capture-MS Homo sapiens
3 EIF3K 27335
Reconstituted Complex Homo sapiens
4 EPB41 2035
Affinity Capture-MS Homo sapiens
Invitro Homo sapiens
Invivo Homo sapiens
Two-hybrid Homo sapiens
5 MPP6 51678
Two-hybrid Homo sapiens
6 GC20  
Affinity Capture-MS Homo sapiens
7 EIF3C 8663
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Invitro Homo sapiens
Invivo Homo sapiens
8 EIF3D 8664
Invitro Homo sapiens
Invivo Homo sapiens
9 EIF5 1983
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
10 EIF3H 8667
Invitro Homo sapiens
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
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