Experiment description of studies that identified BARD1 in exosomes

1 Experiment ID 234 ISEV standards ✔ EM EV Biophysical techniques ✔ TSG101|Alix|HSC70|GAPDH EV Cytosolic markers ✘ EV Membrane markers ✔ HSP90B1 EV Negative markers ✔ qNano EV Particle analysis Identified molecule mRNA Identification method RNA Sequencing PubMed ID 26054723     Organism Homo sapiens Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N Journal name Carcinogenesis Publication year 2015 Sample Hepatocellular carcinoma cells Sample name HKCI-C3 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.13-1.19 g/mL Molecules identified in the study Protein RNA Methods used in the study Western blotting Mass spectrometry RT-PCR RNA Sequencing

Protein-protein interactions for BARD1

Protein Interactor ExoCarta ID Identification method PubMed Species 1 XIST   Co-localization Homo sapiens 2 CSTF1 1477 Invivo Homo sapiens Invivo Homo sapiens Invitro Homo sapiens Invitro Homo sapiens Two-hybrid Homo sapiens Two-hybrid Homo sapiens Reconstituted Complex Homo sapiens Reconstituted Complex Homo sapiens Two-hybrid Homo sapiens 3 FANCD2   Co-localization Homo sapiens Reconstituted Complex Homo sapiens 4 CRSP6   Co-purification Homo sapiens 5 MSH6 2956 Reconstituted Complex Homo sapiens 6 MSH2 4436 Invitro Homo sapiens Reconstituted Complex Homo sapiens 7 NFKBIA   Two-hybrid Homo sapiens 8 BRCA1 672 Invivo Homo sapiens Invivo Homo sapiens Invitro Homo sapiens Invitro Homo sapiens Two-hybrid Homo sapiens Two-hybrid Homo sapiens Two-hybrid Homo sapiens Reconstituted Complex Homo sapiens Reconstituted Complex Homo sapiens Reconstituted Complex Homo sapiens Co-localization Homo sapiens Co-localization Homo sapiens Affinity Capture-Western Homo sapiens Reconstituted Complex Homo sapiens Co-purification Homo sapiens Reconstituted Complex Homo sapiens Co-purification Homo sapiens Affinity Capture-Western Homo sapiens Reconstituted Complex Homo sapiens FRET Homo sapiens Reconstituted Complex Homo sapiens Reconstituted Complex Homo sapiens Affinity Capture-Western Homo sapiens Affinity Capture-Western Homo sapiens Reconstituted Complex Homo sapiens Affinity Capture-MS Homo sapiens Reconstituted Complex Homo sapiens Reconstituted Complex Homo sapiens Two-hybrid Homo sapiens Affinity Capture-Western Homo sapiens Co-localization Homo sapiens Co-localization Homo sapiens Two-hybrid Homo sapiens Co-crystal Structure Homo sapiens Co-purification Homo sapiens Reconstituted Complex Homo sapiens Affinity Capture-Western Homo sapiens Co-purification Homo sapiens Reconstituted Complex Homo sapiens Two-hybrid Homo sapiens Reconstituted Complex Homo sapiens Affinity Capture-Western Homo sapiens Affinity Capture-Western Homo sapiens Co-purification Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-Western Homo sapiens 9 HIST2H2AB 317772 Reconstituted Complex Homo sapiens 10 UBE3A 7337 Reconstituted Complex Homo sapiens 11 RELA   Affinity Capture-Western Homo sapiens 12 BCL3   Two-hybrid Homo sapiens Reconstituted Complex Homo sapiens 13 POU2F1   Invivo Homo sapiens 14 MSH3 4437 Reconstituted Complex Homo sapiens 15 BRE 9577 Affinity Capture-MS Homo sapiens Affinity Capture-Western Homo sapiens 16 UBE2D2 7322 Reconstituted Complex Homo sapiens 17 BRCA2 675 Reconstituted Complex Homo sapiens Co-purification Homo sapiens 18 BARD1 580 Reconstituted Complex Homo sapiens Reconstituted Complex Homo sapiens 19 CXorf53   Affinity Capture-MS Homo sapiens Affinity Capture-Western Homo sapiens 20 TP53   Affinity Capture-Western Homo sapiens Affinity Capture-MS Homo sapiens 21 UBE2D1   Reconstituted Complex Homo sapiens Reconstituted Complex Homo sapiens Reconstituted Complex Homo sapiens Reconstituted Complex Homo sapiens Reconstituted Complex Homo sapiens Reconstituted Complex Homo sapiens Reconstituted Complex Homo sapiens Reconstituted Complex Homo sapiens 22 NPM1 4869 Affinity Capture-MS Homo sapiens Reconstituted Complex Homo sapiens Affinity Capture-Western Homo sapiens 23 UBE1   Reconstituted Complex Homo sapiens Reconstituted Complex Homo sapiens Reconstituted Complex Homo sapiens Reconstituted Complex Homo sapiens Reconstituted Complex Homo sapiens Reconstituted Complex Homo sapiens Reconstituted Complex Homo sapiens 24 EWSR1 2130 Invivo Homo sapiens Invitro Homo sapiens Affinity Capture-Western Homo sapiens Reconstituted Complex Homo sapiens FRET Homo sapiens Two-hybrid Homo sapiens 25 CSTF2   Affinity Capture-Western Homo sapiens Co-purification Homo sapiens Affinity Capture-Western Homo sapiens Co-purification Homo sapiens 26 RAD51   Affinity Capture-MS Homo sapiens Affinity Capture-Western Homo sapiens 27 RBBP8   Invivo Homo sapiens Invitro Homo sapiens 28 H2AFX 3014 Reconstituted Complex Homo sapiens Reconstituted Complex Homo sapiens View the network

Pathways in which BARD1 is involved

No pathways found