Experiment description of studies that identified SH2D4A in sEVs

1 Experiment ID 363 MISEV standards ✘ Biophysical techniques ✔ TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1 Enriched markers ✔ DCLK1 Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 33991177     Organism Homo sapiens Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M." Journal name Proteomics Publication year 2021 Sample Gastric cancer cells Sample name MKN1 - 100K pellet Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

2 Experiment ID 364 MISEV standards ✘ Biophysical techniques ✔ TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1 Enriched markers ✔ DCLK1 Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 33991177     Organism Homo sapiens Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M." Journal name Proteomics Publication year 2021 Sample Gastric cancer cells Sample name MKN1 - 100K pellet Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

3 Experiment ID 365 MISEV standards ✘ Biophysical techniques ✔ TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1 Enriched markers ✔ DCLK1 Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 33991177     Organism Homo sapiens Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M." Journal name Proteomics Publication year 2021 Sample Gastric cancer cells Sample name MKN1 - 100K pellet Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

Protein-protein interactions for SH2D4A

Protein Interactor ExoCarta ID Identification method PubMed Species 1 MORC2   Affinity Capture-MS Homo sapiens 2 CHMP2A 27243 Co-fractionation Homo sapiens 3 MORN3   Two-hybrid Homo sapiens 4 CCDC88B 283234 Two-hybrid Homo sapiens 5 PPP1CB 5500 Affinity Capture-MS Homo sapiens Two-hybrid Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Proximity Label-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Two-hybrid Homo sapiens Affinity Capture-MS Homo sapiens 6 RINT1 60561 Two-hybrid Homo sapiens Two-hybrid Homo sapiens 7 DBNL 28988 Two-hybrid Homo sapiens Two-hybrid Homo sapiens Two-hybrid Homo sapiens 8 SH3GL1 6455 Two-hybrid Homo sapiens Two-hybrid Homo sapiens Two-hybrid Homo sapiens Two-hybrid Homo sapiens 9 SH3RF1   Two-hybrid Homo sapiens 10 FCHO1   Two-hybrid Homo sapiens 11 MILR1   Affinity Capture-MS Homo sapiens 12 STX11 8676 Two-hybrid Homo sapiens 13 PPP1CC 5501 Affinity Capture-MS Homo sapiens 14 PLEKHG4   Two-hybrid Homo sapiens 15 CEP70   Two-hybrid Homo sapiens Two-hybrid Homo sapiens 16 NECAB2   Two-hybrid Homo sapiens Two-hybrid Homo sapiens 17 NDRG1 10397 Co-fractionation Homo sapiens 18 CADPS   Two-hybrid Homo sapiens 19 ANKRD49   Affinity Capture-MS Homo sapiens 20 KRT40   Two-hybrid Homo sapiens Two-hybrid Homo sapiens 21 PPP1R7 5510 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 22 TFIP11   Two-hybrid Homo sapiens 23 MTUS2 23281 Two-hybrid Homo sapiens Two-hybrid Homo sapiens 24 TUBA3C 7278 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 25 FSD2 123722 Two-hybrid Homo sapiens Two-hybrid Homo sapiens 26 GDA 9615 Affinity Capture-MS Homo sapiens 27 CASK 8573 Two-hybrid Homo sapiens Two-hybrid Homo sapiens Two-hybrid Homo sapiens 28 GOLGA6L9   Two-hybrid Homo sapiens 29 ALAS1   Two-hybrid Homo sapiens 30 CCDC172   Two-hybrid Homo sapiens 31 STAM2 10254 Two-hybrid Homo sapiens 32 PPP1CA 5499 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 33 ZNF133   Affinity Capture-MS Homo sapiens 34 SORBS2   Two-hybrid Homo sapiens 35 ZBTB16   Two-hybrid Homo sapiens 36 CDR2L   Two-hybrid Homo sapiens 37 GOLGA2 2801 Two-hybrid Homo sapiens Two-hybrid Homo sapiens 38 UBFD1 56061 Co-fractionation Homo sapiens 39 ZNF655   Two-hybrid Homo sapiens 40 MEOX2   Two-hybrid Homo sapiens 41 BICD2 23299 Proximity Label-MS Homo sapiens 42 TACC1 6867 Two-hybrid Homo sapiens Two-hybrid Homo sapiens 43 CEP76   Two-hybrid Homo sapiens 44 PRKAR1B   Two-hybrid Homo sapiens 45 HCLS1 3059 Two-hybrid Homo sapiens Two-hybrid Homo sapiens Two-hybrid Homo sapiens 46 CALML3 810 Affinity Capture-MS Homo sapiens 47 PHLPP1   Proximity Label-MS Homo sapiens 48 MINK1 50488 Affinity Capture-MS Homo sapiens 49 ZBTB2 57621 Affinity Capture-MS Homo sapiens 50 RABGGTA 5875 Affinity Capture-MS Homo sapiens 51 MAGEA11   Two-hybrid Homo sapiens Affinity Capture-Western Homo sapiens 52 ATP5F1 515 Affinity Capture-MS Homo sapiens 53 CTTN 2017 Affinity Capture-MS Homo sapiens 54 PGAM4 441531 Affinity Capture-MS Homo sapiens 55 HMBOX1   Two-hybrid Homo sapiens Two-hybrid Homo sapiens 56 IKZF1   Two-hybrid Homo sapiens 57 GPRASP2   Two-hybrid Homo sapiens 58 NXF1 10482 Affinity Capture-RNA Homo sapiens 59 LZTS2 84445 Two-hybrid Homo sapiens 60 PSTPIP1 9051 Two-hybrid Homo sapiens 61 Cep72   Affinity Capture-MS Mus musculus 62 SLAIN2   Affinity Capture-MS Homo sapiens 63 FCHSD2   Two-hybrid Homo sapiens 64 AHR 196 Affinity Capture-MS Homo sapiens 65 SUV39H2 79723 Affinity Capture-MS Homo sapiens 66 MRFAP1   Two-hybrid Homo sapiens 67 TRIM27   Two-hybrid Homo sapiens 68 ARC   Two-hybrid Homo sapiens 69 SORBS3 10174 Two-hybrid Homo sapiens Two-hybrid Homo sapiens 70 MAGEA6   Two-hybrid Homo sapiens Two-hybrid Homo sapiens 71 USHBP1   Two-hybrid Homo sapiens 72 Ppp1cb 19046 Affinity Capture-MS Mus musculus 73 HDGF 3068 Co-fractionation Homo sapiens 74 CFAP53   Two-hybrid Homo sapiens 75 NUTM1   Two-hybrid Homo sapiens Two-hybrid Homo sapiens 76 TMEM51 55092 Affinity Capture-MS Homo sapiens 77 TRAF2 7186 Two-hybrid Homo sapiens 78 GRB2 2885 Two-hybrid Homo sapiens Two-hybrid Homo sapiens 79 ALDH9A1 223 Co-fractionation Homo sapiens 80 BLOC1S2 282991 Two-hybrid Homo sapiens 81 VPS52 6293 Two-hybrid Homo sapiens 82 ASNS 440 Co-fractionation Homo sapiens 83 TBC1D9   Affinity Capture-MS Homo sapiens 84 MRPS18C   Affinity Capture-MS Homo sapiens 85 NINL   Two-hybrid Homo sapiens 86 WWOX 51741 Two-hybrid Homo sapiens 87 PNMA1 9240 Two-hybrid Homo sapiens Two-hybrid Homo sapiens 88 UBXN11   Two-hybrid Homo sapiens 89 TF 7018 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 90 PRDM5   Affinity Capture-MS Homo sapiens 91 IDE 3416 Co-fractionation Homo sapiens 92 SKAP1 8631 Two-hybrid Homo sapiens 93 TRIML2   Two-hybrid Homo sapiens 94 SPAG5 10615 Two-hybrid Homo sapiens 95 TRIM23 373 Two-hybrid Homo sapiens 96 TRIM31   Affinity Capture-MS Homo sapiens 97 CDR2   Two-hybrid Homo sapiens Two-hybrid Homo sapiens 98 LDOC1   Two-hybrid Homo sapiens Two-hybrid Homo sapiens 99 PDE4DIP 9659 Two-hybrid Homo sapiens 100 MBD3L1   Two-hybrid Homo sapiens 101 DTD1 92675 Co-fractionation Homo sapiens 102 HSF2BP   Two-hybrid Homo sapiens 103 WARS 7453 Co-fractionation Homo sapiens 104 ZNF774   Two-hybrid Homo sapiens 105 YPEL2 388403 Two-hybrid Homo sapiens View the network

Pathways in which SH2D4A is involved

No pathways found