ExoCarta: Gene summary

Credits | Contact

Gene description for ARHGEF6
Gene name	Rac/Cdc42 guanine nucleotide exchange factor (GEF) 6
Gene symbol	ARHGEF6
Other names/aliases	COOL2 Cool-2 MRX46 PIXA alpha-PIX alphaPIX
Species	Homo sapiens
Database cross references - ARHGEF6
ExoCarta	ExoCarta_9459
Vesiclepedia	VP_9459
Entrez Gene	9459
HGNC	685
MIM	300267
UniProt	Q15052
ARHGEF6 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells	36408942
Bone marrow mesenchymal stem cells	36408942
Bone marrow mesenchymal stem cells	36408942
Bone marrow mesenchymal stem cells	36408942
Thymus	23844026
Urine	21595033
Urine	21595033
Urine	21595033

Gene ontology annotations for ARHGEF6

Molecular Function
	guanyl-nucleotide exchange factor activity	GO:0005085	IBA
	guanyl-nucleotide exchange factor activity	GO:0005085	NAS
	guanyl-nucleotide exchange factor activity	GO:0005085	TAS
	GTPase activator activity	GO:0005096	NAS
	protein binding	GO:0005515	IPI
Biological Process
	apoptotic process	GO:0006915	NAS
	JNK cascade	GO:0007254	NAS
	lamellipodium assembly	GO:0030032	IBA
	lamellipodium assembly	GO:0030032	ISS
Subcellular Localization
	cytoplasm	GO:0005737	IBA
	cytosol	GO:0005829	TAS
	cell-cell junction	GO:0005911	IEA
	lamellipodium	GO:0030027	IBA
	lamellipodium	GO:0030027	ISS

Experiment description of studies that identified ARHGEF6 in exosomes

Experiment ID

489

MISEV standards
✔ EM	Biophysical techniques
✔ Cd9\|Cd81\|Cd63\|Gapdh\|Sdcbp\|Lamp1\|Aqp1\|Rab5a\|Icam1\|Cd82\|Itga2b\|Tsg101\|Lamp2\|Rab35\|Flot1\|Flot2\|Cd151\|Rab5b\|Tfrc\|Uchl1	Enriched markers
✔ Canx	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Rattus norvegicus

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."

Journal name

Proteomics

Publication year

2023

Sample

Bone marrow mesenchymal stem cells

Sample name

BMSC - Passage 6

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

490

MISEV standards
✔ EM	Biophysical techniques
✔ Cd9\|Cd81\|Cd63\|Gapdh\|Sdcbp\|Lamp1\|Aqp1\|Rab5a\|Icam1\|Cd82\|Itga2b\|Tsg101\|Lamp2\|Rab35\|Flot1\|Flot2\|Cd151\|Rab5b\|Tfrc\|Uchl1	Enriched markers
✔ Canx	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Rattus norvegicus

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."

Journal name

Proteomics

Publication year

2023

Sample

Bone marrow mesenchymal stem cells

Sample name

BMSC - Passage 7

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

491

MISEV standards
✔ EM	Biophysical techniques
✔ Cd9\|Cd81\|Cd63\|Gapdh\|Sdcbp\|Lamp1\|Aqp1\|Rab5a\|Icam1\|Cd82\|Itga2b\|Tsg101\|Lamp2\|Rab35\|Flot1\|Flot2\|Cd151\|Rab5b\|Tfrc\|Uchl1	Enriched markers
✔ Canx	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Rattus norvegicus

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."

Journal name

Proteomics

Publication year

2023

Sample

Bone marrow mesenchymal stem cells

Sample name

BMSC - Passage 8

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

492

MISEV standards
✔ EM	Biophysical techniques
✔ Cd9\|Cd81\|Cd63\|Gapdh\|Sdcbp\|Lamp1\|Aqp1\|Rab5a\|Icam1\|Cd82\|Itga2b\|Tsg101\|Lamp2\|Rab35\|Flot1\|Flot2\|Cd151\|Rab5b\|Tfrc\|Uchl1	Enriched markers
✔ Canx	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Rattus norvegicus

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."

Journal name

Proteomics

Publication year

2023

Sample

Bone marrow mesenchymal stem cells

Sample name

BMSC - Passage 9

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

217

MISEV standards
✔ EM	Biophysical techniques
✔ TSG101\|CD81\|CD9\|CD63	Enriched markers
✘	Negative markers
✔ NTA	Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry

PubMed ID

23844026

Organism

Homo sapiens

Experiment description

Characterization of human thymic exosomes.

Authors

"Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."

Journal name

PLoS One

Publication year

2013

Sample

Thymus

Sample name

Normal-Thymus

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Mass spectrometry

Experiment ID

193

MISEV standards
✔ EM	Biophysical techniques
✔ CD63\|CD9	Enriched markers
✔ PHB	Negative markers
✘	Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry

PubMed ID

21595033

Organism

Homo sapiens

Experiment description

Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.

Authors

"Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."

Journal name

Proteomics

Publication year

2011

Sample

Urine

Sample name

Urine - Normal

Isolation/purification methods

Differential centrifugation
Ultracentrifugation
Sucrose density gradient

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Mass spectrometry

Experiment ID

194

MISEV standards
✘	Biophysical techniques
✘	Enriched markers
✘	Negative markers
✘	Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry

PubMed ID

21595033

Organism

Homo sapiens

Experiment description

Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.

Authors

"Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."

Journal name

Proteomics

Publication year

2011

Sample

Urine

Sample name

Urine - Patients of basement membrane nephropathy

Isolation/purification methods

Differential centrifugation
Ultracentrifugation
Sucrose density gradient

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Mass spectrometry

Experiment ID

195

MISEV standards
✘	Biophysical techniques
✘	Enriched markers
✘	Negative markers
✘	Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry

PubMed ID

21595033

Organism

Homo sapiens

Experiment description

Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.

Authors

"Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."

Journal name

Proteomics

Publication year

2011

Sample

Urine

Sample name

Urine - Patients of early IgA nephropathy

Isolation/purification methods

Differential centrifugation
Ultracentrifugation
Sucrose density gradient

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Mass spectrometry

Protein-protein interactions for ARHGEF6

Protein Interactor

ExoCarta ID

Identification method

PubMed

Species

YWHAH

7533

Affinity Capture-MS

Homo sapiens

ATG16L1

55054

Affinity Capture-MS

Homo sapiens

ARHGEF7

8874

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-Western		Homo sapiens
Affinity Capture-MS		Homo sapiens

P4HB

5034

Affinity Capture-MS

Homo sapiens

NUTM1

Two-hybrid

Homo sapiens

SSBP1

6742

Affinity Capture-MS

Homo sapiens

MECP2

4204

Affinity Capture-MS

Homo sapiens

YWHAG

7532

Affinity Capture-MS

Homo sapiens

ARRB2

409

Affinity Capture-MS

Homo sapiens

ABLIM1

3983

Affinity Capture-MS

Homo sapiens

TP53BP2

Two-hybrid

Homo sapiens

PKLR

5313

Affinity Capture-Western

Homo sapiens

SLAIN2

Affinity Capture-MS

Homo sapiens

USP7

7874

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

TRAF2

7186

Two-hybrid

Homo sapiens

CBFA2T3

Two-hybrid

Homo sapiens

TGFBR2

7048

Affinity Capture-Western

Homo sapiens

ICAM1

3383

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

MED4

29079

Affinity Capture-MS

Homo sapiens

VPS37C

55048

Affinity Capture-MS

Homo sapiens

PAK1

5058

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Two-hybrid		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

GOLGA2

2801

Two-hybrid

Homo sapiens

TMEM108

Two-hybrid

Homo sapiens

PABPN1

8106

Affinity Capture-MS

Homo sapiens

GIT2

9815

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Two-hybrid		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

PAK2

5062

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Two-hybrid		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

SIRPA

140885

Reconstituted Complex

Homo sapiens

PFDN1

5201

Affinity Capture-MS

Homo sapiens

SLC9A3R2

9351

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

NCKIPSD

51517

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

XRN2

22803

Affinity Capture-MS

Homo sapiens

PNMA1

9240

Two-hybrid

Homo sapiens

PDIA4

9601

Affinity Capture-MS

Homo sapiens

RABGGTB

5876

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

CCDC33

80125

Two-hybrid

Homo sapiens

PRKDC

5591

Affinity Capture-Western		Homo sapiens
Affinity Capture-Western		Homo sapiens

GPRASP2

Two-hybrid

Homo sapiens

LPXN

9404

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

NCK2

8440

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

CDC42

998

Affinity Capture-Western		Homo sapiens
Co-localization		Homo sapiens

TGFBR1

7046

Affinity Capture-Western

Homo sapiens

SH3GL3

Two-hybrid

Homo sapiens

NT5DC3

51559

Affinity Capture-MS

Homo sapiens

CAPZB

832

Affinity Capture-MS

Homo sapiens

TMEM154

Affinity Capture-MS

Homo sapiens

POU6F2

Two-hybrid

Homo sapiens

TAX1BP3

30851

Affinity Capture-MS

Homo sapiens

ARHGAP44

Affinity Capture-MS

Homo sapiens

GIT1

28964

Affinity Capture-Western		Homo sapiens
Two-hybrid		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

PRDX3

10935

Affinity Capture-MS

Homo sapiens

CBL

867

Affinity Capture-Western		Homo sapiens
Affinity Capture-Western		Homo sapiens

KIF23

9493

Affinity Capture-MS

Homo sapiens

PXN

5829

Proximity Label-MS

Homo sapiens

SYNC

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

CIT

11113

Affinity Capture-MS

Homo sapiens

ZNF580

Two-hybrid

Homo sapiens

KRT27

342574

Two-hybrid

Homo sapiens

NCK1

4690

Affinity Capture-MS

Homo sapiens

PPP1R9B

84687

Affinity Capture-MS

Homo sapiens

CDKN2A

1029

Affinity Capture-MS

Homo sapiens

TRIM54

Two-hybrid

Homo sapiens

SCRIB

23513

Affinity Capture-MS

Homo sapiens

ARHGEF6

9459

Affinity Capture-Western		Homo sapiens
Affinity Capture-Western		Homo sapiens

FBXO28

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

ADAM15

8751

Reconstituted Complex

Homo sapiens

RAC1

5879

Affinity Capture-MS

Homo sapiens

Kalrn

Affinity Capture-MS

Rattus norvegicus

HSF2BP

Two-hybrid

Homo sapiens

PRDX5

25824

Affinity Capture-MS

Homo sapiens

RBM38

Affinity Capture-MS

Homo sapiens

ARHGEF9

Affinity Capture-MS

Homo sapiens

RANGAP1

5905

Affinity Capture-MS

Homo sapiens

CSNK2A2

1459

Affinity Capture-MS

Homo sapiens

SKP1

6500

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

HBA2

3040

Affinity Capture-MS

Homo sapiens

HBB

3043

Affinity Capture-MS

Homo sapiens

View the network

Pathways in which ARHGEF6 is involved

Pathway	Evidence	Source
CDC42 GTPase cycle	TAS	Reactome
Cell death signalling via NRAGE, NRIF and NADE	TAS	Reactome
Cell junction organization	TAS	Reactome
Cell-Cell communication	TAS	Reactome
Cell-extracellular matrix interactions	TAS	Reactome
Death Receptor Signaling	TAS	Reactome
G alpha (12/13) signalling events	TAS	Reactome
G beta:gamma signalling through CDC42	TAS	Reactome
G-protein beta:gamma signalling	TAS	Reactome
GPCR downstream signalling	TAS	Reactome
NRAGE signals death through JNK	TAS	Reactome
p75 NTR receptor-mediated signalling	TAS	Reactome
RAC1 GTPase cycle	TAS	Reactome
Regulation of cytoskeletal remodeling and cell spreading by IPP complex components	TAS	Reactome
RHO GTPase cycle	TAS	Reactome
RHOU GTPase cycle	TAS	Reactome
Signal Transduction	TAS	Reactome
Signaling by GPCR	TAS	Reactome
Signaling by Rho GTPases	TAS	Reactome
Signaling by Rho GTPases, Miro GTPases and RHOBTB3	TAS	Reactome

Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here