Gene description for PATL1
Gene name protein associated with topoisomerase II homolog 1 (yeast)
Gene symbol PATL1
Other names/aliases Pat1b
hPat1b
Species Homo sapiens
 Database cross references - PATL1
ExoCarta ExoCarta_219988
Vesiclepedia VP_219988
Entrez Gene 219988
HGNC 26721
MIM 614660
UniProt Q86TB9  
 PATL1 identified in sEVs derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for PATL1
Molecular Function
    RNA binding GO:0003723 HDA
    RNA binding GO:0003723 IBA
    RNA binding GO:0003723 IDA
    protein binding GO:0005515 IPI
    poly(U) RNA binding GO:0008266 IDA
    poly(G) binding GO:0034046 IDA
Biological Process
    deadenylation-dependent decapping of nuclear-transcribed mRNA GO:0000290 IBA
    deadenylation-dependent decapping of nuclear-transcribed mRNA GO:0000290 IDA
    P-body assembly GO:0033962 IBA
    P-body assembly GO:0033962 IDA
Subcellular Localization
    P-body GO:0000932 IBA
    P-body GO:0000932 IDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    PML body GO:0016605 IEA
    nuclear speck GO:0016607 IEA
    CCR4-NOT complex GO:0030014 IDA
    cytoplasmic ribonucleoprotein granule GO:0036464 IDA
 Experiment description of studies that identified PATL1 in sEVs
1
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PATL1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 LSM2 57819
Two-hybrid Homo sapiens
2 SART1 9092
Co-fractionation Homo sapiens
3 DDX6 1656
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
4 NAGK 55577
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 Rbm14  
Two-hybrid Mus musculus
6 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
7 MAPRE1 22919
Proximity Label-MS Homo sapiens
8 Edc4  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
9 KLC2 64837
Co-fractionation Homo sapiens
10 ITSN1 6453
Affinity Capture-MS Homo sapiens
11 RBPMS 11030
Two-hybrid Homo sapiens
12 LSM1 27257
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
13 XPO1 7514
Affinity Capture-MS Homo sapiens
14 DMRTB1  
Two-hybrid Homo sapiens
15 PPIE 10450
Affinity Capture-MS Homo sapiens
16 WWOX 51741
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
17 ANAPC2 29882
Proximity Label-MS Homo sapiens
18 TARDBP 23435
Affinity Capture-MS Homo sapiens
19 KRT8 3856
Proximity Label-MS Homo sapiens
20 CAMK2A 815
Affinity Capture-MS Homo sapiens
21 CLK2 1196
Two-hybrid Homo sapiens
22 CDC37 11140
Co-fractionation Homo sapiens
23 HNRNPA2B1 3181
Affinity Capture-MS Homo sapiens
24 TAS2R42  
Affinity Capture-MS Homo sapiens
25 PARK2  
Affinity Capture-MS Homo sapiens
26 RC3H2  
Affinity Capture-MS Homo sapiens
27 LSM4 25804
Affinity Capture-MS Homo sapiens
28 FHL3 2275
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
29 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
30 HOOK3 84376
Proximity Label-MS Homo sapiens
31 QPRT 23475
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 TSC1 7248
Two-hybrid Homo sapiens
33 ARHGAP18 93663
Affinity Capture-MS Homo sapiens
34 CPEB1  
Proximity Label-MS Homo sapiens
35 APP 351
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
36 LSM5 23658
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 GOLGA2 2801
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
38 PURG  
Affinity Capture-MS Homo sapiens
39 PFN1 5216
Proximity Label-MS Homo sapiens
40 MAPRE3  
Proximity Label-MS Homo sapiens
41 ACACA 31
Positive Genetic Homo sapiens
42 TOP2A 7153
Co-fractionation Homo sapiens
43 LSM7  
Affinity Capture-MS Homo sapiens
44 CTCFL  
Affinity Capture-MS Homo sapiens
45 PRPF3  
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
46 PACSIN3 29763
Affinity Capture-MS Homo sapiens
47 RPA3 6119
Proximity Label-MS Homo sapiens
48 LSM3 27258
Affinity Capture-MS Homo sapiens
49 PSMA3 5684
Two-hybrid Homo sapiens
50 SF3A1 10291
Co-fractionation Homo sapiens
51 NTRK1 4914
Affinity Capture-MS Homo sapiens
52 CSNK2A2 1459
Affinity Capture-MS Homo sapiens
53 CLTA 1211
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 DCP1A 55802
Affinity Capture-MS Homo sapiens
55 KRTAP6-2  
Two-hybrid Homo sapiens
56 HSPA1A 3303
Affinity Capture-MS Homo sapiens
57 NFKBID  
Two-hybrid Homo sapiens
58 BICD2 23299
Proximity Label-MS Homo sapiens
59 LSM6 11157
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
60 PRPF4 9128
Co-fractionation Homo sapiens
61 PML 5371
Affinity Capture-MS Homo sapiens
62 Pef1  
Two-hybrid Mus musculus
63 RC3H1 149041
Affinity Capture-MS Homo sapiens
64 KRT19 3880
Proximity Label-MS Homo sapiens
65 Cenpe  
Affinity Capture-MS Mus musculus
66 KRT31 3881
Two-hybrid Homo sapiens
67 AKAP1 8165
Proximity Label-MS Homo sapiens
68 MYO18A 399687
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
69 EP300 2033
Affinity Capture-MS Homo sapiens
70 PHF20  
Co-fractionation Homo sapiens
71 IGHM 3507
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
72 LRFN4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
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