Gene description for GORASP2
Gene name golgi reassembly stacking protein 2, 55kDa
Gene symbol GORASP2
Other names/aliases GOLPH6
GRASP55
GRS2
p59
Species Homo sapiens
 Database cross references - GORASP2
ExoCarta ExoCarta_26003
Entrez Gene 26003
HGNC 17500
MIM 608693
UniProt Q9H8Y8  
 GORASP2 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Thymus 23844026    
 Gene ontology annotations for GORASP2
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    mitotic cell cycle GO:0000278 TAS
    organelle organization GO:0006996 IMP
Subcellular Localization
    membrane GO:0016020 TAS
    Golgi membrane GO:0000139 TAS
    Golgi apparatus GO:0005794 IDA
 Experiment description of studies that identified GORASP2 in exosomes
1
Experiment ID 235
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 217
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
CD81|CD9|CD63
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for GORASP2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PCBD1 5092
Two-hybrid Homo sapiens
2 TMED2 10959
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
3 TSC22D4  
Two-hybrid Homo sapiens
4 GOLGA2 2801
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
5 MIF 4282
Two-hybrid Homo sapiens
6 BLZF1  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
7 TGFA  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
8 TMED3  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
9 TMED10 10972
Reconstituted Complex Homo sapiens
10 PRPS1 5631
Two-hybrid Homo sapiens
11 TRAF2  
Two-hybrid Homo sapiens
12 NME1 4830
Two-hybrid Homo sapiens
13 NQO2 4835
Two-hybrid Homo sapiens
14 RAD54B  
Two-hybrid Homo sapiens
15 CBLB  
Two-hybrid Homo sapiens
16 RAB2A 5862
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
17 RAB1A 5861
Affinity Capture-MS Homo sapiens
Invivo Homo sapiens
18 NUP62 23636
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which GORASP2 is involved
PathwayEvidenceSource
Golgi Cisternae Pericentriolar Stack Reorganization TAS Reactome





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